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Staphylococcal Protein A

IMS02-008-333  |  Clonality: Polyclonal  |  Host: Hen  |  Reactivity: Staphylococcus aureus

Staphylococcal Protein A in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (IMS02-008-333)

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Product Information

Immunogen

Purified, recombinant Protein A from GE Healthcare, product number 17-0872 

Host Chicken
Clonality Polyclonal
Purity Affinity purified IgY.
Format Liquid in 0.9% NaCl, 0.1% sodium azide. pH 7.2
Quantity 100 µl (1 mg/ml)
Storage Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications Affinity purified IgY
Recommended dilution 1 : 500 as a capture antibody (ELISA), 1 : 2000 as a detection antibody (ELISA), 1: 1000 (WB)
Expected | apparent MW

55 | 47 kDa

Reactivity

Confirmed reactivity Staphylococcus aureus
Predicted reactivity Staphylococcus aureus
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

The IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation.

This antibody can be used as capture and detection antibody in ELISA. as a detection ab, a dilution 1:2000 and can detect Protein A below 1ng/ml or 1ppm (where ppm is the concentration of Protein A relative to the concentration of IgG, e.g. 1 ng/mL Protein A in 1mg/mL of IgG equals 1 ppm).

This antibody is detecting both recombinant and native Protein A.

One precipitation arc in immunodiffusion against Staphylococcal Protein A.

Related products

Related products

IMS02-109-334 | Anti-Streptococcal Protein G, chicken antibodies

IMS02-136-331 | Anti-Peptostreptococcus magnus Protein L, chicken antibodies

AS15 2849 | Anti-Staphyloccocus aureus, mouse antibodies

Secondary antibodies

Background

Background

Protein A is a surface protein of S. aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface, which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.

Product citations

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