Staphylococcal Protein A
IMS02-008-333 | Clonality: Polyclonal | Host: Hen | Reactivity: Staphylococcus aureus
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Purified, recombinant Protein A from GE Healthcare, product number 17-0872
55 | 47 kDa
The IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation.
This antibody can be used as capture and detection antibody in ELISA. as a detection ab, a dilution 1:2000 and can detect Protein A below 1ng/ml or 1ppm (where ppm is the concentration of Protein A relative to the concentration of IgG, e.g. 1 ng/mL Protein A in 1mg/mL of IgG equals 1 ppm).
This antibody is detecting both recombinant and native Protein A.
Protein A is a surface protein of S. aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface, which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
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