Staphylococcal Protein A

Product no: IMS02-008-333

IMS02-008-333  |  Clonality: Polyclonal  |  Host: Hen  |  Reactivity: Staphylococcus aureus

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Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    Purified, recombinant Protein A from GE Healthcare, product number 17-0872 

    Host: Chicken
    Clonality: Polyclonal
    Purity: Immunogen affinity purified IgY in 0.9% NaCl, 0,1% sodium azide., pH 7.2.
    Format: Liquid
    Quantity: 100 µl (1 mg/ml)
    Storage: Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1 : 500 as a capture antibody (ELISA), 1 : 2000 as a detection antibody (ELISA), 1: 1000 (WB)
    Expected | apparent MW:

    55 | 47 kDa

  • Reactivity
  • Confirmed reactivity: Staphylococcus aureus
    Predicted reactivity: Staphylococcus aureus
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information:

    The IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation.

    This antibody can be used as capture and detection antibody in ELISA. as a detection ab, a dilution 1:2000 and can detect Protein A below 1ng/ml or 1ppm (where ppm is the concentration of Protein A relative to the concentration of IgG, e.g. 1 ng/mL Protein A in 1mg/mL of IgG equals 1 ppm).

    This antibody is detecting both recombinant and native Protein A.

    Additional information (application): One precipitation arc in immunodiffusion against Staphylococcal Protein A
  • Background
  • Background:

    Protein A is a surface protein of S. aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface, which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.

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AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

Now available only as separate products:
1 x AS16 ECL-N
1 x AS16 ECL-S

This product can be purchased in 3 different volumes:

AS16 ECL-SN-10, 10 ml. Trial size limited to one per customer

AS16 ECL-SN, 100 ml

AS16 ECL-SN-1L, 1L

Choose the appropriate volume in the drop down menu to the right

From 26 €