Name:
Phone:
E-mail:
Address:


 


SurA | SurA

AS10 798   | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Yersinia pseudotuberculosis

SurA | SurA in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (AS10 798)

DATA SHEET IN PDF

Qty: 
447
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

 

Product Information

Immunogen

Recombinant SurA over-expressed as a N-terminal fusion with His(x6)-tag, which was removed prior to immunization. UniProt protein accession number B1JKY1; NCBI annotated locus tag YPK_3571

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

47.7 | 52 kDa

Reactivity

Confirmed reactivity Yersinia pseudotuberculosis
Predicted reactivity Yersinia enterocolitica, Yersinia pestis
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

 Approximately 10 µg of total protein from Y. pseudotuberculosis    extracted with   were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following  transfer in milk solution (10 % milk powder in TBST) for 1 h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 5 000 overnight at  4 °C with agitation. The antibody solution was decanted and the blot was washed twiced for 15 min in TBST at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from GE Healthcare) diluted to 1: 20 000 in  for 1h at room temperature with agitation. The blots were washed as above and developed for 35 min with homemade ECL  detection reagent. Exposure time was 60  seconds.

 

western blot using anti-SurA antibody
 

Additional information

In WB, lower molecular weight cross-reacting bands exist at approximately 34 kDa and 24 kDa. Neither of these interfere with SurA identification.

Related products

Related products

secondary antibodies

Background

Background

SurA (stationary phase survival) is a periplasmic chaperone and folding catalyst with peptidyl-prolyl cis-trans isomerase activity. It is involved in the maintenance of Gram-negative bacterial envelope integrity and the transport/assembly of a subset of OMPs.

Product citations

Related products: SurA | SurA

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 25 €
AS09 607 Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
199 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
194 €