SurA | SurA
AS10 798 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Yersinia pseudotuberculosis
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Product Information
Recombinant SurA over-expressed as a N-terminal fusion with His(x6)-tag, which was removed prior to immunization. UniProt protein accession number B1JKY1; NCBI annotated locus tag YPK_3571
47.7 | 52 kDa
Reactivity
Application examples
application example Approximately 10 µg of total protein from Y. pseudotuberculosis extracted with were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in milk solution (10 % milk powder in TBST) for 1 h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 5 000 overnight at 4 °C with agitation. The antibody solution was decanted and the blot was washed twiced for 15 min in TBST at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from GE Healthcare) diluted to 1: 20 000 in for 1h at room temperature with agitation. The blots were washed as above and developed for 35 min with homemade ECL detection reagent. Exposure time was 60 seconds. |
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Additional information
In WB, lower molecular weight cross-reacting bands exist at approximately 34 kDa and 24 kDa. Neither of these interfere with SurA identification.
Background
SurA (stationary phase survival) is a periplasmic chaperone and folding catalyst with peptidyl-prolyl cis-trans isomerase activity. It is involved in the maintenance of Gram-negative bacterial envelope integrity and the transport/assembly of a subset of OMPs.
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