SurA | SurA
AS10 798 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Yersinia pseudotuberculosis
|Recommended dilution||1 : 5000 (WB)|
|Expected | apparent MW||
47.7 | 52 kDa
|Confirmed reactivity||Yersinia pseudotuberculosis|
|Predicted reactivity||Yersinia enterocolitica, Yersinia pestis|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
In WB, lower molecular weight cross-reacting bands exist at approximately 34 kDa and 24 kDa. Neither of these interfere with SurA identification.
to be added when available
Approximately 10 µg of total protein from Y. pseudotuberculosis extracted with were separated on 12 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in milk solution (10 % milk powder in TBST) for 1 h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 5 000 overnight at 4 °C with agitation. The antibody solution was decanted and the blot was washed twiced for 15 min in TBST at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from GE Healthcare) diluted to 1: 20 000 in for 1h at room temperature with agitation. The blots were washed as above and developed for 35 min with homemade ECL detection reagent. Exposure time was 60 seconds.
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