THP | Allopregnanolone steroid standard

AS04 041S | GMP manufactured steroid standard

Product Information

Format Lyophilized

Quantity 10 mg

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Protect from light, Can be stored for 1 month at 4°C in a sealed tube or flask.

Tested applications Radioimmunoassay (RIA)

Reactivity

Application examples

Additional information

Additional information Allopregnanolone standard is made under GMP conditions and thus has the purity standards required for human usage in clinical trials, Produced by Umecrine AB

The lyophilized powder of this standard is not soluble in water.

Standard dissolving instruction:

Use ethanol (99.5 %) or methanol to prepare a concentrated (stock) solution of THP standard. This solution can be stored for longer time in a sealed tube.
Working solution can be used daily but needs to be changed after one month.
Working solution is used to prepare standard points 0-200 pg. It needs to be dried under nitrogen before it is diluted in water based soltion, otherwise the steroid will oxidase.
Following dyring, water based solution can be added to this standard.

Background

Allopregnanolone, (3alpha-hydroxy-20-oxo-5alphapregnan-11alpha-yl-carboxymethylether), also known as 3alpha,5alpha-tetrahydroprogesterone or THP, a neuroactive steroid secreted by the mammalian ovary, exerts its anesthetic, anxiolytic, and sedative/hypnotic effects through potentiation of GABA A receptors. It is produced in the human corpus luteum and their release is stimulated by trophic hormone.

Product citations

Selected references Lee at al. (2014). Progesterone and allopregnanolone improve stroke outcome in male mice via distinct mechanisms but neither promotes neurogenesis. J of Neurochemistry, DOI: 10.1111/jnc.12990.

Related products: THP | Allopregnanolone steroid standard

THP | Allopregnanolone

AS04 041 | Clonality: Polyclonal | Host: Hen | Reactivity: All...

424 €

background:	Allopregnanolone, (3alpha-hydroxy-20-oxo-5alphapregnan-11alpha-yl-carboxymethylether), also known as 3alpha,5alpha-tetrahydroprogesterone or THP, a neuroactive steroid secreted by the mammalian ovary, exerts its anesthetic, anxiolytic, and sedative/hypnotic effects through potentiation of GABA A receptors. It is produced in the human corpus luteum and their release is stimulated by trophic hormone.
All references:	Lee at al. (2014). Progesterone and allopregnanolone improve stroke outcome in male mice via distinct mechanisms but neither promotes neurogenesis. J of Neurochemistry, DOI: 10.1111/jnc.12990.
Format:	Lyophilized
Quantity:	10 mg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Protect from light, Can be stored for 1 month at 4°C in a sealed tube or flask.
tested applications:	Radioimmunoassay (RIA)
additional information:	Allopregnanolone standard is made under GMP conditions and thus has the purity standards required for human usage in clinical trials, Produced by Umecrine AB
additional information (application):	The lyophilized powder of this standard is not soluble in water. Standard dissolving instruction: Use ethanol (99.5 %) or methanol to prepare a concentrated (stock) solution of THP standard. This solution can be stored for longer time in a sealed tube. Working solution can be used daily but needs to be changed after one month. Working solution is used to prepare standard points 0-200 pg. It needs to be dried under nitrogen before it is diluted in water based soltion, otherwise the steroid will oxidase. Following dyring, water based solution can be added to this standard.