showForm Anti-TIP2;1 (delta) | Delta-VM23 antibodies

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TIP2;1 (delta) | Delta-VM23

AS09 494 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Raphanus sativus

TIP2;1 (delta) | Delta-VM23 in the group Antibodies for Plant/Algal  / Membrane Transport System / Vacuolar membrane at Agrisera AB (Antibodies for research) (AS09 494)

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Product Information

Immunogen

KLH-synthetic peptide conserved in Raphanus sativus TIP2;1 (delta) O82142

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 8000 (ELISA), 1 : 1000 (WB)
Expected | apparent MW

24.9 | 23 kDa

Reactivity

Confirmed reactivity Raphanus sativus
Predicted reactivity Raphanus sativus
Not reactive in

Arabidopsis thaliana, Pisum sativum

Application examples

Application examples

Application example

western blot using anti-TIP2;1 antibodies

crude membrane of yeast cells expressing recombinaTIP2;1 (RsTIP2;1) and TIP1;1 (RsTIP1;1) proteins were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-TIP2;1 antibodies (AS09 494, 1:2000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.
Left panel: antibody incubacted without peptide, right panel with peptide used to elicit this antibody (peptide competition assay)

Additional information

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few  µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

42 kDa dimer is also detected by those antibodies.

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.

Related products

Related products

collection of antibodies to tonoplast proteins

AS09 492 | anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)

AS09 493 | anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)

AS09 510 | anti-TIP2;1 | tonoplast intrinistic protein 2-1

AS09 511 | anti-TIP2;2 | tonoplast intrinistic protein 2-2 (C-terminal)

Plant protein extraction buffer

Background

Background

TIPs belong to MIP/aquaporin protein family. Alternative name of radish TIP2;1 (delata) is delta-VM23.

Product citations

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