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TIP2;2 | tonoplast intrinistic protein 2-2, C-terminal

AS09 511 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Oryza sativa

TIP2;2 | tonoplast intrinistic protein 2-2, C-terminal in the group Antibodies for Plant/Algal  / Membrane Transport System / Vacuolar membrane at Agrisera AB (Antibodies for research) (AS09 511)

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Product Information

Immunogen

KLH-conjugated peptide derived from C-terminus of Oryza sativa TIP2-2 Q5Z6F0

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 8000 (ELISA), 1 : 2000 (WB)
Expected | apparent MW

25 | 20-24 kDa

Reactivity

Confirmed reactivity Oryza sativa
Predicted reactivity

Oryza sativa

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

western blot using anti-TIP2;2 C-terminal antibodies

1.5 µg of crude membrane fraction/lane from Oryza sativa  L. cv. Akitakomachi leaf blade and root were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-TIP2;2 C-terminal antibodies (AS09 511, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Peptide used to elicti this antibody has been added to primary antibody incubation (right panel, + +) and therefore specific signal has been depleted in this neutralization/immunocompetition assay.

Additional information

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few  µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Triton X-100 should not be included in the protein extraction buffer, when cell organelles or membrane proteins must be separated from soluble proteins. Because, Triton X breaks membrane structure and solubilizes most membranes proteins. Furthermore, it should be noted that Triton X at high concentrations binds SDS and mask the detergent effect of SDS for SDS-PAGE. Also, micelles of Triton X behave as a large complex with molecular mass of 90 kDa at high concentrations in SDS-PAGE.

Related products

Related products

collection of antibodies to tonoplast proteins

AS09 492 | anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)

AS09 493 | anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)

AS09 494 | anti-TIP2;1 (delta) | Delta-VM23

AS09 510 | anti-TIP2;1 | tonoplast intrinistic protein 2-1

AS09 510 | anti-TIP2;2 | tonoplast intrinistic protein 2-2

AS09 511 | anti-TIP2;2 | tonoplast intrinistic protein 2-2 (C-terminal)

Plant protein extraction buffer

Background

Background

TIP2-2 belongs to aquaporin protein family and facilitates transport of water and small neutral solutes across tonoplast membrane.

Product citations

Selected references Sakurai et al. (2008). Tissue and cell-specific localization of rice aquaporins and their water transport activities- Plant Cell Physiol. 49: 30-39.

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