TNFa | Tumor necrosis factor alpha

IMS01-102-339 | Clonality: Polyclonal | Host: Hen | Reactivity: Human


product information
Background		Tumor Necrosis Factor alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
Immunogen		purified full length recombinant protein P01375
Host		Chicken
Clonality		Polyclonal
Clone
Purity		Total IgY
Format		Liquid in 0.9% NaCl, 0.1% sodium azide
Quantity		100 µl
Reconstitution
Storage		Store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications		Total, purified IgY
Related products		IMS06-102-325 \| anti-TNFa \| Tumor necrosis factor alpha, Biotin conjugated, chicken antibodies IMS01-142-310 \| anti-Etanercept (Enbrel^®), recombinant human tumor necrosis factor receptor, chicken antibodies
Additional information		The IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation.

application information
Recommended dilution		1 : 5000 (WB)
Expected \| apparent MW		26 kDa
Confirmed reactivity		Human
Predicted reactivity		Cat, Dog, Horse, Pig, Primates
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		This antibody, in dilution 1:5 000, will detect 10 ng of SDS denatured and reduced human TNFa on western blots. ELISA performance: Purified antigen is precoated on the microtitre plate. IMS01-102-339 is used as primary antibody at various dilutions. ALP-conjugated anti-chicken IgG is used as a secondary antibody at a dilution of 1:1000.
Selected references		to be added when available

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