IKP

TNFa | Tumor necrosis factor alpha, Biotin conjugated

Product no: IMS06-102-325

IMS06-102-325  |  Clonality: Polyclonal  |  Host: Chicken  |  Reactivity: Human

348
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    purified full length recombinant protein P01375

    Host: Chicken
    Clonality: Polyclonal
    Purity: Immunogen affinity purified IgY in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide., pH 7.2.
    Format: Liquid
    Quantity: 100 l
    Storage: Store at 4C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1 : 5000 (WB)
    Expected | apparent MW:

    26 kDa

  • Reactivity
  • Confirmed reactivity: Human
    Predicted reactivity: Cat, Dog, Horse, Pig, Primates
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: The IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation; labelled with biotin,Total IgY concentration for this antibody is 15 mg/ml
    Additional information (application): The antibody, in dilution 1:5 000, will detect 10 ng of SDS denatured and reduced human TNFa on western blots
  • Background
  • Background:

    Tumor Necrosis Factor alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.

  • Reviews:
  • To leave a review, you should have previously purchased this product and to your customer profile.

    This product doesn't have any reviews.

Accessories

img missing

IMS01-102-339  |  Clonality: Polyclonal  |  Host: Chicken  |  Reactivity: Human

348 €
Add to cart
Info
img missing

IMS01-142-310  |  Clonality: Polyclonal  |  Host: Hen  |  Reactivity: Human

348 €
Add to cart
Info
Buy 2 items of this product for 267.00 €/items
Buy 3 items of this product for 232.00 €/items
Close