Toc75 | Outer chloroplast membrane translocon complex protein

AS08 292 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Pisum sativum, Synechocystis PCC6803

Toc75 | Outer chloroplast membrane translocon complex protein

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Toc75 protein sequence of Pisum sativum (Q43715)

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

88 | 75 kDa (for Pisum sativum)

Reactivity

Confirmed reactivity Pisum sativum, Synechocystis sp. PCC6803

Predicted reactivity Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Background

Import of protein precurors into chloroplasts occurs via translocon complexes at the outer (Toc/TOC complex) and inner (Tic/TIC complex) envelope membrane. The major components of the Toc (TOC) complex in plants are Toc75, Toc34 and Toc159 (formerly Toc86). Toc75 (OEP75) forms the main import pore. It is an abundant protein in the outer envelope membrane of chloroplasts from expanding tissues but it is also found in plastids of roots, stems and flowers. Alternative names: 75 kDa translocon at the outer-envelope membrane of chloroplasts, import intermediate-associated protein of 75 kDa, 75 kDa chloroplast outer envelope protein,

Product citations

Selected references Ulrich et al. (2012).Chloroplast β-BarrelProteins AreAssembled into theMitochondrialOuterMembrane in a Process That Depends on the TOM and TOB Complexes. J. Biol. Chem. August 10.

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Confirmed reactivity:	Pisum sativum, Synechocystis sp. PCC6803
predicted reactivity:	Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
calculated \| apparent molecular mass [kDa]:	88 \| 75 kDa (for Pisum sativum)
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH-conjugated synthetic peptide derived from Toc75 protein sequence of Pisum sativum (Q43715)
Purity:	Serum
Quantity:	50 µl
recommended dilution:	1 : 1000 (WB)
Reconstitution:	For reconstitution add 50 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
All references:	Ulrich et al. (2012).Chloroplast β-BarrelProteins AreAssembled into theMitochondrialOuterMembrane in a Process That Depends on the TOM and TOB Complexes. J. Biol. Chem. August 10.
background:	Import of protein precurors into chloroplasts occurs via translocon complexes at the outer (Toc/TOC complex) and inner (Tic/TIC complex) envelope membrane. The major components of the Toc (TOC) complex in plants are Toc75, Toc34 and Toc159 (formerly Toc86). Toc75 (OEP75) forms the main import pore. It is an abundant protein in the outer envelope membrane of chloroplasts from expanding tissues but it is also found in plastids of roots, stems and flowers. Alternative names: 75 kDa translocon at the outer-envelope membrane of chloroplasts, import intermediate-associated protein of 75 kDa, 75 kDa chloroplast outer envelope protein,