Tubulin gamma chain (monoclonal antibodies)

AS20 4482 | Clonality: Monoclonal | Host: Mouse | Reactivity: Human, Protozoa, Plants

Product Information

Immunogen KLH-conjugated gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449. UniProt: P23258
The epitope was located in the aminoacid sequence PDYISW (aa441-446 in human), which is identical for gamma-tubulin 1 and gamma-tubulin 2.

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG1

Purity Purified by Protein A affinity chromatography in PBS with 15 mM sodium azide.

Format Liquid

Quantity 100 µg

Storage Store at 4°C; Do not freeze.
Do not exceed exipry date is provided on the tube.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Immunocytochemistry (ICC), Western blot (WB)

Recommended dilution 2-8 µg/ml (ICC), 1 µg/ml (WB)

Expected | apparent MW

51 kDa

Reactivity

Confirmed reactivity human, protozoa

Predicted reactivity Plants
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

This antibody recognizes C-terminus (amino acids 434-449 in human) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC).
The epitope which this antibody is recognizing is conserved in Arabidopsis thaliana Tubulin gamma-1 chain, UniProt: P38557, Gene ID: At3g61650 and Tubulin gamma-2 chain, UniProt: P38558, Gene ID: At5g05620

Recommended secondary antibody: goat anti-mouse IgG1 AS16 3715

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Background

Tubulin gamma chain is essential for the control of microtubular network. It is found at microtubule organizing centers (MTOC) such as the spindle poles