V-ATPase, A | vacuolar H+-ATPase subunit A

AS09 502 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Acetabularia sp. Hordeum vulgare, Oryza sativa, Pinus sylvestris, Pyrus sp. , Ricinus communis

V-ATPase, A | vacuolar H+-ATPase subunit A in the group Antibodies Plant/Algal  / Membrane Transport System / Vacuolar membrane at Agrisera AB (Antibodies for research) (AS09 502)
V-ATPase, A | vacuolar H+-ATPase subunit A


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Product Information


Purified native V-ATPase subunit A from Ricinus communis, UniProt: B9RHV0

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 8000 (ELISA), 1 : 2000 (WB)
Expected | apparent MW

63 | 68 kDa (Ricinus communis)


Confirmed reactivity Acetabularia sp. Hordeum vulgare, Oryza sativa, Pinus sylvestris, Pyrus sp. , Ricinus communis
Predicted reactivity Arabidopsis thaliana, Cucumis sativus, Gossypium mexicanum, Hordeum vulgare, Populus trichocarpa, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

Application example

western blot using anti-V-ATPase subunit A antibodies

65 µg/lane of purified vacuolar membranes from Vigna radiata L. (1,3)and purified V-ATPase, 7.4 µg/lane(2,4)  were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-V-ATPase subunit A antibodies (AS09 502, 1:2000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

CBB - staining with Coomasie blue - left panel. Immunoblot - western blot detectoin - right panel.

Additional information

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few  µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Related products

Related products

collection of antibodies to vacuolar proteins

AS09 467 | Anti-V-ATPase subunit A | vacuolar H+-ATPase, rabbit antibodies

AS09 503 | Anti-V-ATPase, B | vacuolar ATP synthase subunit beta, rabbit antibodies

AS09 468 | Anti-V-ATPase subunit c | vacuolar H+-ATPase, subunit c (16 kDa), rabbit antibodies

ASS09 497 | Anti- V-ATPase subunit D |V-type proton ATPase subunit D, rabbit antibodies

AS07 213 | Anti-V-ATPase subunit E of tonoplast H+ATPase, rabbit antibodies

AS09 499 | Anti-V-ATPase subunit H |V-type proton ATPase subunit H, rabbit antibodies

Plant protein extraction buffer



V-ATPase subunit A is a catalytic subunit of V1 complex of vacuolar ATPase. This enzyme (EC= is involved in acidification process of various compartements of eucaryotic cell.  This protein is coded by VHA-A gene.

Alternative names: Vacuolar proton pump subunit alpha, vacuolar H(+)-ATPase subunit A, V-ATPase 69 kDa subunit

Product citations

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