IKP

V-ATPase, D | Vacuolar H+-ATPase subunit D

Product no: AS09 497

AS09 497 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide conserved in Arabidopsis thaliana V-ATPase subunit D UniProt: Q9XGM1, TAIR: At3g58730

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 100 l
    Reconstitution: For reconstitution add 100 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1 : 8000 (ELISA), 1 : 2000 (WB)
    Expected | apparent MW:

    29 | 29 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Sorghum bicolor, Zea mays
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information:

    0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

    Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

    Additional information (application):

    Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

    Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

  • Background
  • Background:

    V-ATPase is an enzyme responsible for acidification of intracellular compartments. Subunit D is the peripheral subunit of V1 complex.
    Alternative name: Vacuolar proton pump subunit D, vacuolar H(+)-ATPase subunit D

  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection



    Method for isolation of plant vacuolar membranes

    method for vacuolar membrane isolation

    Courtesy of Dr. Masayoshi Maeshima, Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences Nagoya University Nagoya, Japan

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