V-ATPase, D | Vacuolar H+-ATPase subunit D

AS09 497 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

V-ATPase, D | Vacuolar H+-ATPase subunit D in the group Antibodies Plant/Algal  / Membrane Transport System / Vacuolar membrane at Agrisera AB (Antibodies for research) (AS09 497)
V-ATPase, D | Vacuolar H+-ATPase subunit D


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Product Information


KLH-conjugated synthetic peptide conserved in Arabidopsis thaliana V-ATPase subunit D UniProt: Q9XGM1, TAIR: At3g58730

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 8000 (ELISA), 1 : 2000 (WB)
Expected | apparent MW

29 | 29 kDa


Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Sorghum bicolor, Zea mays
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Related products

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collection of antibodies to vacuolar proteins

AS09 466 | Anti-V-ATPase subunit a | vacuolar H+ ATPase, subunit a, rabbit antibodies

AS09 467 | Anti-V-ATPase subunit A | vacuolar H+-ATPase, rabbit antibodies

AS09 503 | Anti-V-ATPase, B | vacuolar ATP synthase subunit beta, rabbit antibodies

AS09 468 | Anti-V-ATPase subunit c | vacuolar H+-ATPase, subunit c (16 kDa) , rabbit antibodies

ASS09 497 | Anti- V-ATPase subunit D |V-type proton ATPase subunit D, rabbit antibodies

AS07 213 | Anti-V-ATPase subunit E of tonoplast H+ATPase, rabbit antibodies

AS09 499 | Anti-V-ATPase subunit H |V-type proton ATPase subunit H, rabbit antibodies

Plant protein extraction buffer



V-ATPase is an enzyme responsible for acidification of intracellular compartments. Subunit D is the peripheral subunit of V1 complex.
Alternative name: Vacuolar proton pump subunit D, vacuolar H(+)-ATPase subunit D

Product citations

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