V5 (mouse antibody, monoclonal, clone V5.E10)
AS15 3031 | Clonality: monoclonal | Host: Mouse | Reactivity: V5-tagged proteins
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Product Information
Immunogen
KLH-conjugated GKPIPNPLLGLDST synthetic peptide
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgG1
Purity
Protein A purified
Format
Liquid in PBS, pH 7.4, with 0.05% sodium azide
Quantity
100 µg
Storage
Store at -20°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications
ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 5000-1 : 10 000 (ELISA), 1 : 50-1 : 100 (IHC) , 1 : 500-1 : 1000 (WB)
Reactivity
Confirmed reactivity
V5-tagged fusion proteins
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
Exact working dilution for WB, ELISA and IHC needs to be determined by end user.
Antibody is affinity purified.
Background
Background
V5-tag is a tag that can be added to a protein of interest as a fusion protein to enable purification and detection.
Product citations
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