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Zip7 | Zinc transporter protein Zip7

AS08 318  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Hordeum vulgare

Zip7 | Zinc transporter protein Zip7 in the group Antibodies for Plant/Algal  / Developmental Biology / Ion metabolism at Agrisera AB (Antibodies for research) (AS08 318)

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Product Information

Immunogen

KLH-conjugated two synthetic peptides from the large loop region of barley ZIP7 Q257D6

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 10 000 (ELISA), 1 : 2000 (WB)
Expected | apparent MW

40 kDa

Reactivity

Confirmed reactivity Hordeum vulgare
Predicted reactivity

Oryza sativa, Triticum aestivum, Zea mays

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

 

 
 
 
 
 
 
The blotting showed two bands at approx 30 kDa  and 12 kDa. A Nickel column-purified recombinant protein,  representing a cytoplasmic loop region of the ZIP7 protein,  was run on the gel. It was expressed in E. coli with a  HIS tag, and is 81 amino acids long, which equates to  the ~12-15 kDa band seen in the elution fractions of the western blot. The immunogens used were synthetic peptides  targeted to two regions within this cytoplasmic loop.
AS08 318 western picture.jpg

Additional information

Related products

Background

Background

HvZIP7 (zinc regulated ZRT/IRT-like transporter protein 7) is a plasma membrane bound protein potentially involved in Zn homeostasis in plants.

Product citations

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