Zip7 | Zinc transporter protein Zip7
AS08 318 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Hordeum vulgare
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Product Information
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
100 µl
Reconstitution
For reconstitution add 100 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
ELISA (ELISA), Western blot (WB)
Recommended dilution
1 : 10 000 (ELISA), 1 : 2000 (WB)
Expected | apparent MW
40 kDa
Reactivity
Confirmed reactivity
Hordeum vulgare
Predicted reactivity
Oryza sativa, Triticum aestivum, Zea mays
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Application examples
application example
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The blotting showed two bands at approx 30 kDa and 12 kDa. A Nickel column-purified recombinant protein, representing a cytoplasmic loop region of the ZIP7 protein, was run on the gel. It was expressed in E. coli with a HIS tag, and is 81 amino acids long, which equates to the ~12-15 kDa band seen in the elution fractions of the western blot. The immunogens used were synthetic peptides targeted to two regions within this cytoplasmic loop.
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Additional information
Background
Background
HvZIP7 (zinc regulated ZRT/IRT-like transporter protein 7) is a plasma membrane bound protein potentially involved in Zn homeostasis in plants.
Product citations
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