Zip7 | Zinc transporter protein Zip7
AS08 318 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Hordeum vulgare
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Oryza sativa, Triticum aestivum, Zea mays
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The blotting showed two bands at approx 30 kDa and 12 kDa. A Nickel column-purified recombinant protein, representing a cytoplasmic loop region of the ZIP7 protein, was run on the gel. It was expressed in E. coli with a HIS tag, and is 81 amino acids long, which equates to the ~12-15 kDa band seen in the elution fractions of the western blot. The immunogens used were synthetic peptides targeted to two regions within this cytoplasmic loop.
HvZIP7 (zinc regulated ZRT/IRT-like transporter protein 7) is a plasma membrane bound protein potentially involved in Zn homeostasis in plants.