Zip7 | Zinc transporter protein Zip7

AS08 318 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Hordeum vulgare

Product Information

Immunogen

KLH-conjugated two synthetic peptides from the large loop region of barley ZIP7 Q257D6

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1 : 10 000 (ELISA), 1 : 2000 (WB)

Expected | apparent MW

40 kDa

Reactivity

Confirmed reactivity Hordeum vulgare

Predicted reactivity

Oryza sativa, Triticum aestivum, Zea mays

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Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

application example

The blotting showed two bands at approx 30 kDa and 12 kDa. A Nickel column-purified recombinant protein, representing a cytoplasmic loop region of the ZIP7 protein, was run on the gel. It was expressed in E. coli with a HIS tag, and is 81 amino acids long, which equates to the ~12-15 kDa band seen in the elution fractions of the western blot. The immunogens used were synthetic peptides targeted to two regions within this cytoplasmic loop.