Protocols

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• Preparation of immunoaffinity columns by antibody coupling
• How to elute antibodies from affinity columns?
• Alternative affinity purification of antibodies, low amount of antigen available

Western Blot troubleshooting Immunoprecipitation troubleshooting

Short ELISA protocol ELISA troubleshooting

IgY protocols: How to purify IgY? How to work with IgY - egg yolk antibodies? Western Blot using IgY Immunoprecipitation using IgY Immunohistochemistry using IgY Delipidation of egg yolk

IgG, IgM: IgG - general info How to work with IgM?

How to store IgG or IgY antibodies? Affinity purified antibodies are precipiatating... How to dissolve a peptide? Most common reasons for protein inactivation

How to remove cross-reacting anti-KLH antibodies? Problems with dialysis

Using Antibodies: A Laboratory Manual, E.Harlow and D.Lane, 1999,
ISBN: 0879695447; Publisher: Cold Spring Harbor Laboratory Press.

Antibody Resource Page

Molecular Biology Methods Forum