Name:
Phone:
E-mail:
Message

GST class-phi | Glutathione S tranferase

AS09 479 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, L. formosa, S. superba

20% price reduction as of January 2020

GST class-phi | Glutathione S tranferase in the group Antibodies for Plant/Algal  / Membrane Transport System / Endomembrane system at Agrisera AB (Antibodies for research) (AS09 479 )

DATA SHEET IN PDF

Qty: 
381
Buy 2 items of this product for 339.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Datasheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana UniProt: P46422, TAIR: At4g02520

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 8000 (ELISA), 1 : 1000 (WB)
Expected | apparent MW

24 | 26 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Liquidambar formosa, Schima superba
Predicted reactivity Arabidopsis lyRatha, Brassica juncea, Brassica rapa, Bruguiera gymnorhiza, Capsella rubella,Capsicum annuum, Capsicum chinense, Gossypium hirsutum, Hyoscyamus muticus, Nicotiana sylvestris, Noccaea caerulescens, Physcomitrella patens, Solanum commersonii, Solanum tuberosum, Vigna radiata var. radiata
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

 

 

western blot detection using anti-GST antibodies

1 µg and 10 µg of crude membrane fraction/lane from Arabidopsis thaliana were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrene box and probed with anti-GST antibodies (AS09 479, 1:1000, 1h) and secondary anti-rabbit (1:1000, 1 h). Detection was done using chemiluminescence. All steps were performed in RT with agitation.

Additional information

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufacture by Operon Biotechnologies.

Related products

Background

Background

Glutathione S-transferase PM24 (EC=2.5.1.18) is an enzyme which performs conjugation of reduced glutathione to a range of exogenous and endogenous hydrophobic compunds. Alternative name: 24 kDa auxin-binding protein, glutathione S-transferse PM24

Product citations

Selected references Kumar and Chattopadhyay (2015). Changes in the proteome of pad2-1, a glutathione depleted Arabidopsis mutant, during Pseudomonas syringae infection.
Chen et al. (2013). Photosynthetic and antioxidant responses of Liquidambar formosana and Schima superba seedlings to sulfuric-rich and nitric-rich simulated acid rain. Plant Physiol & Biochem.

Related products: GST class-phi | Glutathione S tranferase

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
From 15 €
AS09 607 Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
195 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
190 €
AS06 183  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: ...
373 €