IKP

BoNT-A | Botulinum Neurotoxin A (Heavy Chain Binding Domain)

Name: BoNT-A | Botulinum Neurotoxin A (Heavy Chain Binding Domain)
Brand: IKP
SKU: AS20 4465
Price: 476 EUR
Availability: InStock

Product no: AS20 4465

AS20 4465 | Clonality: Polyclonal | Host: Chicken | Reactivity: Botulinum Neurotoxin A

476 €

DATA SHEET IN PDF

Delivery: 3-6 business days

Product Info

Immunogen:	Purified Botulinum Neurotoxin Type A (Heavy Chain Binding Domain)
Host:	Chicken
Clonality:	Polyclonal
Purity:	Immunogen affinity purified in PBS pH 7.2. Contains 0.075% sodium azide.
Format:	Liquid
Quantity:	0,1 mg
Storage:	Store at -20�C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	ELISA (ELISA), Western blot (WB)
Recommended dilution:	1:10 000 (ELISA), 1:2000 (WB)
Expected \| apparent MW:	97 kDa

Reactivity
Confirmed reactivity: Botulinum toxin A heavy chain
Additional Information

Additional information (application):

For direct ELISA coating with 2 µg of Botulinium Toxin A is recommended combined with primary antibody dilution of 1: 10 000.

For Western blot 0.5 µg of non reduced holotoxin or heavy chains is recommended together with 1: 2000 dilution of a primary antibody.

Background

Background:

Botulinum toxin is a toxin produced by the anaerobic, gram-positive, bacterium of the genus Clostridium (C. botulinum, C. butyricum, C. baratii and C. argentinense). These strains are widely distributed and can be found in soil and dust. Eight types of botulinum toxin are distinguished, named type A–H. Type A and B are capable of causing disease in humans (botulism) and have longest activity in vivo, and are also used commercially (BOTOX) and medically. Types C–G are less common; types E and F can cause disease in humans, while the other types cause disease in other animals. BotA is cleaved into two chains: heavy and light.

Alternative name: Bontoxilysin-A

Protocols
Antibody Protocols
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