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Plant/Algal cell antibodies / Mitochondria | Respiration

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Useful references, helpful for mitochondria isolation from:

Chlamydomonas  Isolation, Purification, and Characterization of Mitochondria from Chlamydomonas reinhardtii. Eriksson et al. 1995, Plant Physiol.

Arabidopsis thaliana Preparation of leaf mitochondria from Arabidopsis thaliana Keech et al.  2005, Physiologia Plantarum

Protocol Dr. Thomas Elthon protocol for preparation of crude mitochondrial fraction


Agrisera western blot recommendations and trouble shooting and Video tutorial can be found here.

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ACO1 | aconitase

AS09 521 | clonality:polyclonal | host:rabbit | reactivity: Arabidopsis thaliana, Solanum lycopersicum

304 €
AOX | alternative oxidase

AS10 699 | clonality: monoclonal  |  host: mouse  |  reactivity: higher plants, fungi, protozoa

304 €
AOX1 | alternative oxidase 1

AS06 152  |  clonality: polyclonal  |  host: rabbit  |  reactivity: Chlamydomonas reinhardtii  |  cellular [compartment marker] of mitochondrial inner membrane

304 €
AOX1/2 | plant alternative oxidase 1 and 2

AS04 054  |  clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana, Lupius uteus, Pisum sativum, Solanum tuberosum and T.aestivum, conifers and Physcomitrella patens cellular [compartment marker] of mitochondrial inner membrane

310 €
AtFtsH4 | plant fts metallo-protease H4

AS07 205  |  affinity purified  |  clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana

304 €
AtpB | beta subunit of ATP synthase (10 µl)

AS03 030-10  |  clonality: polyclonal  |  host: hen  reactivity: [global antibody] for plant and bacterial F-type ATP synthases

130 €
AtpB | beta subunit of ATP synthase (10 µl)

AS05 085-10  |  clonality: polyclonal  |  host: rabbit  |  reactivity: [global antibody] for plant and bacterial F-type ATP synthases

130 €
AtpB | beta subunit of ATP synthase (100 µl)

AS03 030  |  clonality: polyclonal  |  host: hen  reactivity: [global antibody] for plant and bacterial F-type ATP synthases

304 €
AtpB | beta subunit of ATP synthase (100 µl)

AS05 085  |  clonality: polyclonal  |  host: rabbit  |  reactivity: [global antibody] for plant, animal and bacterial F-type ATP synthases

310 €
Beta-CA1, beta-CA2 | carbonic anhydrase | mitochondrial | Chlamydomonas

AS11 1737   | clonality: polyclonal  |  host: rabbit  |  reactivity: Chlamydomonas reinhardtii | marker of mitochondria for low carbon dioxide grown cells

304 €
CAH3 | carbonic anhydrase

AS05 073  |  clonality: polyclonal  |  host: rabbit  |  reactivity: Chlamydomonas reinhardtii

304 €
Catalase

AS09 501 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, B. napus, N. bentamina, O.sativa, Z.mays

360 €
COX11 | cytochrome c oxidase assembly protein

AS10 938 | clonality: polyclonal  |  host: rabbit  |  reactivity: Chlamydomonas reinhardtii

Antibody available in March 2012.

COXII | cytochrome oxidase subunit II (200 µg)

AS04 053A-200  |  affinity purified  |  clonality: polyclonal  |  host: rabbit  |  reactivity: eudicots, monocots, Physcomitrella patens  |  cellular [compartment marker] of mitochondrial inner membrane

598 €
COXII | cytochrome oxidase subunit II | Blocking peptide

AS04 053P  |  Blocking peptide

82 €
COXII | cytochrome oxidase subunit II

AS04 052  |  clonality: polyclonal  |  host: hen  |  reactivity:A. thaliana (leaf extract and isolated mitochondria), B. oleracea, P. major, P. euryphylla, S. uniflora, S. dioica

304 €
COXII | cytochrome oxidase subunit II

AS04 053A  |  affinity purified  |  clonality: polyclonal  |  host: rabbit  |  reactivity: eudicots, monocots, Physcomitrella patens  |  cellular [compartment marker] of mitochondrial inner membrane

336 €
COXIIb | cytochrome oxidase subunit II b

AS06 151  |  clonality: polyclonal  |  host: rabbit  |  reactivity: Chlamydomonas reinhardtii  |  cellular [compartment marker] of mitochondrial inner membrane

304 €
Cyt c | cytochrome c

AS08 343  |  clonality: polyclonal  |  host: rabbit  reactivity: Arabidopsis thaliana, Lupinus luteus| marker of PCD

304 €
DHAR1 | Dehydroascorbate Reductase 1

AS11 1746  | clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana

330 €
FtsH1, 3,5,10 | plant ftsh metallo-protease H1+H3 + H5+ H10

AS11 1789  |  clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana

304 €
FtsH10 | plant fts metallo-protease H10

AS07 251  |  clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana

304 €
FtsH3 + FtsH10 | plant fts metallo-protease H3 + H10

AS07 204  |  affinity purified  |  clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana

304 €
GDC-H | H protein of glycine decarboxylase complex (GDC)

AS05 074  |  clonality: polyclonal  |  host: rabbit  |   reactivity: Arabidopsis thaliana  |  cellular [compartment marker] of mitochondrial matrix

304 €
GLDH | galactono-1,4 lactone dehydrogenase

AS06 182  |   clonality: polyclonal  |  host: rabbit  |  reactivity:  H. vulgare, G. max,  A. sativa, Helianthus annuus, Z.mays

304 €
HSP70 | heat shock protein 70, mitochondrial

AS08 347  |  clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana

330 €
Idh | isocitrate dehydrogenase

AS06 203A  |  affinity purified  |  clonality: polyclonal  |  host: rabbit  |  reactivity: A. thaliana, C.annuum, L. esculentum, P. sativum, S. tuberosum, Z. mayscellular [compartment marker] of mitochondrial matrix

330 €
NADH | cytochrome B5 reductase

AS11 1795 | clonality: polyclonal  |  host: rabbit  | predicted reactivity: Populus trichocarpa

330 €
NDPK | nucleaoside diphosphate kinase

AS08 302  |  clonality: polyclonal  | host: rabbit  | reactivity:A.thalina,P.sativum

304 €
SHMT | serine hydroxymethyltransferase

AS05 075  |  clonality: polyclonal  |  host: rabbit  |  reactivity:  A.thaliana, S. oleracea  |  cellular [compartment marker] of mitochondrial matrix

304 €

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