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COXII | Cytochrome oxidase subunit II (affinity purified)

265 €
Buy 2 items of this product for 190 €/each
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AS04 053A  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: eudicots, monocots, Physcomitrella patens  |  Cellular [compartment marker] of mitochondrial inner membrane

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AS04 053A

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product information
Background

Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Alternative name: cytochrome c oxidase subunit 2

Immunogen

KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots includingArabidopsis thaliana AtmG00160, monocots including  Oryza sativa P04373 and Physcomitrella patens Q1XGA9

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Blue Native-PAGE (BN-PAGE), Western blot (WB)
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AS04 052 COXII | cytochrome oxidase subunit II

Antibodies to other mitochondrial proteins


Recommended secondary antibody
Additional information

Cellular [compartment marker] of mitochondrial inner membrane

application information
Recommended dilution 1 : 1000 (BN-PAGE), 1 : 1000 (WB)
Expected | apparent MW

29.4 | 30 kDa (for Arabidopsis thaliana)

Confirmed reactivity Arabidopsis thaliana (leaf extract and isolated mitochondria), Betula nana, Brassica napus, Brassica oleracea, Cicer aretinum, Cucumis melo, Erophorum vaginatum, Hordeum vulgare, Lilium longifolorum, Nicotiana tabacum, Picea abies, Plantago major, Plantago euryphylla, Silene uniflora, Silene dioica, Physcomitrella patens, Triticum aestivum, Triticum durum Desf. , Zea mays, Vicia faba, Quercus rubra
Predicted reactivity Cucumis melo, Glycine max, Oryza sativa, Physcomitrella patens, Pisum sativum, Triticum aestivum
Not reactive in Saccharina japonica
Additional information
Antibody detects COXII protein most optimally in membrane fractions. The signal is weak in a in total protein extract.

Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.
Selected references Nagel et al. (2017). Arabidopsis SH3P2 is an ubiquitin-binding protein that functions together with ESCRT-I and the deubiquitylating enzyme AMSH3. Proc Natl Acad Sci U S A. 2017 Aug 7. pii: 201710866. doi: 10.1073/pnas.1710866114.
Garmash et al. (2017). Expression profiles of genes for mitochondrial respiratory energy-dissipating systems and antioxidant enzymes in wheat leaves during de-etiolation. J Plant Physiol. 2017 Aug;215:110-121. doi: 10.1016/j.jplph.2017.05.023.
Weißenberger et al. (2017). The PPR protein SLOW GROWTH 4 is involved in editing of nad4 and affects the splicing of nad2 intron 1. Plant Mol Biol. 2017 Mar;93(4-5):355-368. doi: 10.1007/s11103-016-0566-4.
Cai et al. (2017). Emp10 encodes a mitochondrial PPR protein that affects the cis-splicing of nad2 intron 1 and seed development in maize. Plant J. 2017 Mar 27. doi: 10.1111/tpj.13551.
Schimmeyer et al. (2016). L-Galactono-1,4-lactone dehydrogenase is an assembly factor of the membrane arm of mitochondrial complex I in Arabidopsis. Plant Mol Biol. 2016 Jan;90(1-2):117-26. doi: 10.1007/s11103-015-0400-4. Epub 2015 Oct 31.
Li et al. (2016). Characterization of a novel β-barrel protein (AtOM47) from the mitochondrial outer membrane of Arabidopsis thaliana. J Exp Bot. 2016 Nov;67(21):6061-6075. Epub 2016 Oct 6.
Pavlovič et al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009.
Long et al. (2015). Contributions of photosynthetic and non-photosynthetic cell types to leaf respiration in Vicia faba L. and their responses to growth temperature. Plant Cell Environ. 2015 Apr 1. doi: 10.1111/pce.12544.
Rurek et al. (2015). Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, organellar translation and ultrastructure. Biochim Biophys Acta. 2015 Jan 21. pii: S0005-2728(15)00016-X. doi: 10.1016/j.bbabio.2015.01.005.
Dimkovikj and Van Hoewyk (2014). Selenite activates the alternative oxidase pathway and alters primary metabolism in Brassica napus roots: evidence of a mitochondrial stress response. BMC Plant Biol. 2014 Sep 30;14:259. doi: 10.1186/s12870-014-0259-6.
Li et al. (2014). AUTOPHAGY-RELATED11 Plays a Critical Role in General Autophagy- and Senescence-Induced Mitophagy in Arabidopsis. Plant Cell. 2014 Feb 21.


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