Animal cell antibodies / Carrier proteins

product information

Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein  has many immunostimulatory properties, including the ability to enhance the host’s immune response by interacting with T cells,  monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.

purified keyhole limpet hemocyanin (KLH), whole molecule,

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Western blot (WB), ELISA (ELISA), immunolocalization (IL)

AS99 002 | anti-BSA rabbit antibody

AS02 023 | anti-BSA hen antibody

protein present in plant vascular tissue (xylem and vascular cambium) is detected by anti-KLH antibodies (Höglund et al. 2002) which might lead to false results in IL when using anti-peptide antibodies generated to KLH-conjugated peptide. Further information about it can be found here.

50 µg of anti-KLH antibody is enough for ca. 50 western blots

application information

1: 10 000 (ELISA), 1: 10 000 (WB), 1: 1000 (IL)

ca. 400 kDa/subunit

Megathura crenulata - most commonly used carrier protein

Megathura crenulata - most commonly used carrier protein

no confirmed exceptions from predicted reactivity known in the moment

Antibody can be used as a negative control to determine if observed signal is generated by anti-KLH or anti-peptide antibodies. Due to its large size KLH protein will be very difficult to separate on SDS-PAGE.

Höglund et al. (2002). An Antigen Expressed During Plant Vascular Development Crossreacts with Antibodies Towards KLH (Keyhole Limpet Hemocyanin). J of Histochem & Cytochem. 50:999-1003.