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gamma-glutamyl-cysteine

397 €

AS11 1811 | clonality: polyclonal | host: rabbit | reactivity:A.thalina, C. pepo, N. tabacum

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AS11 1811

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product information
Background

γ-glutamyl-cysteine is a precursor of glutathione and is synthesized by linkage of glutamate and cysteine by γ-glutamylcysteine synthetase. Synonymes:gamma-glutamyl-cysteine, γ-glutamyl-cysteine. Alternative names: γ-EC, γ-Glu-Cys.

Immunogen

γ-glutamyl-cysteine (gamma-EC) linked by glutaraldehyde

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized
Quantity 2x100 ĩg
Reconstitution For reconstitution add 100 ĩl of sterile water per tube.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications immunocytochemistry (ICC), immunogold (IG)
Related products

other antibodies to proteins involved in response to heavy metals

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 50 (ICC), (IG)

Expected | apparent MW
Confirmed reactivity

Arabidopsis thaliana, Cucurbita pepo L. subsp. peop var. styriaca Greb, Nicotiana tabacum cv. samsun

Predicted reactivity
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information

Immunogold labeling for electron microscopy

Block ultrathin sections (80nm) prepared for immunogold labeling with 2% bovine serum albumine (BSA) in phosphate buffered saline (PBS, pH 7.2). Then treat the sections with the primary antibody (anti-γ-glutamylcysteine rabbit polyclonal IgG) diluted 1:50 in PBS containing 1% BSA for 2 h at room temperature. After a short rinse in PBS (3 X 5 min), incubate samples with a gold-conjugated secondary antibody (goat anti-rabbit IgG; eg. 10nm) diluted 1:50 in PBS including 1% BSA for 90 min at room temperature. After a short wash in PBS (2 X 5 min), and distilled water (3 X 5 min) observe grids under a transmission electron microscope.

Selected references Koffler et al. (2011). Subcellular distribution of glutathione precusors in Arabidopsis thaliana. Journal of Integrative Plant Biology. 53: 930-941.


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