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PIP (PIP1;1, PIP1;2, PIP1;3, PIP1;4, PIP1;5) aquaporins (40 ĩg, ALP-conjugated)

406 €

AS09 487-ALP | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, J. curcas, P. nigra, P. trichocarpa, R. sativus

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AS09 487-ALP

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product information
Background

PIPs proteins are aquaporins which facilitate the transport of water and small neutral molecules across cell membrane. Alternative names: PIP1-1, PIP1-2, PIP1-3, plasma membrane intrinistic protein 1a, 1b, 1c, PIP1a, PIP1b, PIP1c, plasma membrane aquaporin-1, transmembrane protein A, TMP-A, AthH2, transmembrane protein B, TMP-B,

Immunogen

synthetic peptide conserved in Arabidopsis thaliana: PIP1;1 P61837,At3g61430 PIP1;2 Q06611,At2g45960 PIP1;3 Q08733,At1g01620 , PIP1;4 Q39196, At4g00430, PIP1;5 Q8LAA6 At4g23400

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Liquid in PBS pH 7.4, conjugated to ALP
Quantity 40 µg
Reconstitution
Storage

Store at +4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

Tested applications western blot (WB)
Related products

collection of antibodies to plasma membrane proteins

AS09 488 | anti-PIP2;1 | aquaporin PIP2;1

AS09 489 | anti-PIP1;1, PIP1;2, PIP1;3 | aquaporins

AS09 490 | anti-PIP2;2 | plasma membrane aquaporin 2b

AS09 491 | anti-PIP2;1,PIP2;2,PIP2;3 |plasma membrane intrinistic protein 2-1,2-2, 2-3

Plant protein extraction buffer

Secondary antibodies

Additional information

Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protocol for plasma membrane isolation can be found here.

application information
Recommended dilution

1: 1000 with standard ECL (WB)

Expected | apparent MW

30.68 | 28 kDa

Confirmed reactivity

Arabidopsis thaliana, Brassica sp., Jatropha curcas L. cv. "Biji Jarak ", Mesembryantheum crystallinum, Populus nigra, Populus trichocarpa, Raphanus sativus, Thellungiella salsuginea

Predicted reactivity

dicots including: Brassica sp., Vicia faba, monocots: Hordeum vulgare, Oryza sativa, Triticum aestivum, trees: Juglans regia, Populus tremula,

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

 

 

Selected references


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