SCE1 | SUMO-conjugating enzyme SCE1

Name: SCE1 | SUMO-conjugating enzyme SCE1
SKU: AS19 4285
Price: 319 EUR
Availability: InStock

Product no: AS19 4285

AS19 4285 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Zea mays

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Product Info

Immunogen:	Full length, recombinant SCE1 of Arabidopsis thaliana, UniProt: Q42551-1, TAIR: At3g57870 overexpressed in E.coli
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
Reconstitution:	For reconstitution add 50 µl, of sterile water
Storage:	Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
Recommended dilution:	1 : 3000 (WB)
Expected \| apparent MW:	18 \| 18 kDa

Reactivity

Confirmed reactivity:	Arabidopsis thaliana, Zea mays
Predicted reactivity:	Cajanus cajan, Cicer arietinum , Corchorus capsularis, Gossypium hirsutum, Litchi chinensis, Medicago truncatula, Morus notabilis, Nelumbo nucifera, Nicotiana benthamiana, Nicotiana tabacum, Nicotiana sylvestris, Noccaea caerulescens, Phoenix dactylifera, Populus canadensis, Prunus yedoensis , Theobroma cacao, Triticum urartu , Zea mays, Zostera marina, Vigna radiata Species of your interest not listed? Contact us
Not reactive in:	No confirmed exceptions from predicted reactivity are currently known

Background

Background:

SCE1 (SUMO-conjugating enzyme SCE1) is a SUMO-conjugating enzyme which acts by accepting the SUMO proteins from the E1 SUMO-activating heterodimer SAE1/SAE2 and catalyzes its covalent attachment to other proteins with the E3 SUMO ligases SIZ1 and MMS21.

Alternative names: Protein EMBRYO DEFECTIVE 1637, Protein hus5 homolog SUMO-conjugating enzyme 1, AtSCE1.

Product Citations

Selected references:

Saracco et al. (2007). Genetic analysis of SUMOylation in Arabidopsis: conjugation of SUMO1 and SUMO2 to nuclear proteins is essential. Plant Physiol. 2007 Sep;145(1):119-34.

Protocols
Agrisera Western Blot protocol and video tutorials

Protocols to work with plant and algal protein extracts

Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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