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Goat anti-Human kappa chain, Biotin conjugated

216 €
AS10 1404 | clonality: polyclonal | host: goat | reactivity: human kappa chain

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AS10 1404

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product information
Background Goat anti-human kappa (k) chain is a secondary antibody conjugated to biotin, which binds to human kappa chain in immunological assays. Antibody is affinity purified using solid phase human kappa chain.
Immunogen Purified human Kappa (k) Chain BAA37169.1
Host Goat
Clonality Polyclonal
Clone
Purity Affinity purified IgG
Format Liquid
Quantity 0.5 mg
Reconstitution
Storage 2-8 °C. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Related products other secondary antibodies to human immunoglobulins
Tested applications
Additional information

Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.

Based on immunoelectrophoresis, this antibody reacts with: kappa (k) light chains on human immunoglobulins Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin human serum proteins, heavy chains on human immunoglobulins, lambda light chains on human immunoglobulins.

application information
Recommended dilution This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:2000 - 1:20 000 for ELISA/Western blot 1:500 - 1:5 000 for Immunohistochemistry
Selected references Reinhart et al. (2014). In search of expression bottlenecks in recombinant CHO cell lines-a case study. Appl Microbiol Biotechnol. 2014 Feb 21.


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