Protocols

Plant organelle/membrane isolation

• Mitochondrial fraction
• Nuclear fraction
• Plasma membrane fraction
• Thylakoid extraction
• Arabidopsis thylakoid preparation
• Arabidopsis thylakoid extraction
• Arabidopsis lumen extraction
• BBY preparation
• Chlorophyll measurements
• PSII RC extraction for cryo-EM

Plant protein isolation

• Extraction of leaf proteins
• Diatom protein extraction
• Phenol protein extraction
• Protein extraction from grasses
• Ponceau membrane staining
• TCA acetone precipitation method
• Collection of articles

Western blot

• Western blot protocol
• Western blot video tutorial
• Peptide neutralization/competition assay
• Simultaneous Western blot
• Quantitative Western blot
• Quantitative Western blot video tutorial
• Western blot troubleshooting 
• Western blot using IgY
• Western blot in denatured conditions (urea gel)

Other protocols

• Dot blot
• ELISA
• Immunoprecipitation
• Immunoprecipitation/IgY
• Immunohistochemistry
  
• Anti-KLH antibody removal 
• Yolk delipidation
• Meiotic staining

Technical information

Antibody types

• IgG
• IgM
• IgY
• MW and pI for immunoglobulins

Purification

• Antibody purification
• Antibody purification - small amount of protein
• IgY purification methods
• Protein purification using antibodies
• Elution of antibodies from affinity columns

Other

• Antibody storage
• Antibody production guide
• Secondary antibody guide
• Problems with dialysis