Protocols and technical information

Protocols

• ELISA Short protocol & trouble shooting
• Immunoprecipitation Trouble shooting
• Immunoprecipitation/IgY
• Immunohistochemistry
• Neutralization/competition assay
• Anti-KLH antibody removal
• Western blot protocol
• Dot-Blot protocol
• Western blot trouble shooting
• Western Blot using IgY
• Yolk delipidation

Technical information

• IgG
• IgM
• IgY
• IgY purification
• Protein purification using antibodies
• Elution of antibodies from affinity columns
• Problems with dialysis
• Antibody purification - small amount of protein
• Antibody storage
• Antibody production guide

Agrisera video tutorial: How to make a good western blot? (qualitative and quantitative)

Useful basic reference to work with antibodies: Using Antibodies: A Laboratory Manual, E.Harlow and D.Lane, 1999, ISBN: 0879695447; Publisher: Cold Spring Harbor Laboratory Press.