Protocols and technical information

Protocols

ELISA Short protocol & troubleshooting
Immunoprecipitation Troubleshooting
Immunoprecipitation/IgY
Immunohistochemistry
Neutralization/competition assay
Anti-KLH antibody removal
Western blot protocol
Dot-Blot protocol
Western blot troubleshooting
Western blot using IgY
Yolk delipidation

Technical information

IgG
IgM
IgY
IgY purification
Protein purification using antibodies
Elution of antibodies from affinity columns
Problems with dialysis
Antibody purification - small amount of protein
Antibody storage
Antibody production guide

Agrisera video tutorial: How to make a good western blot? (qualitative and quantitative)

Useful, basic reference for working with antibodies: Using Antibodies: A Laboratory Manual, E.Harlow and D.Lane, 1999, ISBN: 0879695447; Publisher: Cold Spring Harbor Laboratory Press.