PSI (Photosystem I)
PSI/PSII ratio can be estimated once a quantitative immunoblot for PsaC (AS10 939) and for either PsbA (AS05 084) or PsbD (AS06 146), is performed in 3-4 replicates. Ratio calculation is done on a sample by sample basis. These ratios are based on the subunits present, whether they are in the assembled photosystem or not. Under some circumstances (eg. high light), there are biological reasons that the subunit numbers do not necessarily match the functional photosystems.
Literature
Brown et al. (2008). Flux capacities and acclimation costs in Trichodesmium from the Gulf of Mexico. Marine Biology 154 (3): 413-422.
Levitan et al. (2010). Regulation of nitrogen metabolism in the marine diazotroph Trichodesmium IMS101 under varying temperatures and atmospheric CO2 concentrations. Environmental Microbiology 12(7): 1899-1912.
Thamatrakoln et al. (2013). A ‘Death-Specific Protein’ in a marine diatom regulates photosynthetic responses to iron and light availability. Proceedings of the National Academy of Science 110(50): 20123-20128 Li G,
Li et al. (2014). The Nitrogen Costs of Photosynthesis in a Diatom under Current and Future pCO2. New Phytologist, 205:533-543.
Protocols and Technical Information • Support • Western Blot Video Tutorials
AS08 300 | Extraction buffer for quantitative isolation of total soluble/membrane protein from plant tissue and algal/bacterial cells, optimized for subsequent western blot detection in denatured conditions
AS06 163 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana | cellular [compartment marker] of plastid stroma
AS15 2872 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Zea mays
AS06 172 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, C. quitensis Kunt Bartl, C. pumilum, C. reinhardtii, C. zofingiensis, F. vesiculosus, H.vulgare, M. polymorpha, N. oceanica, N. tabacum, O. sativa, P. abies, P. sativum, P. strobus, P. vulgaris, S. oleracea, C.reinhardtii, Synechococcus PCC 7942, Synechocystis PCC 6803, Scenedesmus obliquus, microalgae N. gaditana
AS10 695 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, cyanobacteria
Benefits of using this antibody
AS10 939 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, cyanobacteria, diatoms
Benefits of using this antibody
AS22 4730 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
AS09 461 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Plants (monocots and dicots, conifers), moss: Physcomitrella patens, Chlamydomonas reinhardtii, Triticum aestivum, cyanobacteria
compartment marker of thylakoid membrane
AS08 324A | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Hordeum vulgare
AS22 4731 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
AS04 047 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Hordeum vulgare, Oryza sativa
AS22 4732 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
AS06 104 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, N. tabaccum, O. sativa, S. oleracea
AS20 4368 | Clonality: Polyclonal | Host: Chicken | Reactivity: Arabidopsis thaliana
AS06 144 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
AS06 105 | Clonality: Polylclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Nicotiana tabaccum, Spinacia oleracea
AS06 143 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
AS23 4919 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
AS06 108 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, N. tabaccum, S. oleracea
AS06 109 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
AS04 050 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Spinacia oleracea
AS15 3089 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
AS07 273 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii, cyanobacteria
AS07 274 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii, cyanobacteria
Hardcover: 204 pages Publisher: World Scientific Publishing Company (7 Jan. 2019)
Language: English
ISBN-10: 9813223103 ISBN-13: 978-9813223103
Product Number: AS19 4290Book ; Product Dimensions: 15.2 x 1.9 x 22.9 cm
AS04 049 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, N. tabacum, S. oleracea
Replaced by AS23 4919.
The set is suitable for Western blot using samples from a wide range of species making this product suitable for teaching labs or mutant screening.
Benefits of using this antibody set
This discounted antibody set contains the following high titer, well published antibodies and is suitable for Western blot using samples from a wide range of species making. This product suitable for teaching labs or mutant screening.
Benefits of using this antibody set
This set includes three discounted, well published, compartment marker antibodies of your choice. Benefits of using this antibody set
Related products:
SA000002 | Plant Cell Compartment Antibody Marker Set | 5 antibodies
SA000003 | Plant Cell Compartment Antibody Marker Set | 10 antibodies
AS04 042S | Recombinant protein standard for quantitation