PsaN | PSI-N subunit of photosystem I

Name: PsaN | PSI-N subunit of photosystem I
SKU: AS06 109
Price: 302 EUR
Availability: InStock

AS06 109 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

PsaN | PSI-N subunit of photosystem I in the group Antibodies Plant/Algal / Photosynthesis / PSI (Photosystem I) at Agrisera AB (Antibodies for research) (AS06 109)

Product Information

Immunogen

KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana PsaN protein sequence At5g64040

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophikized

Quantity 200 µg

Reconstitution For reconstitution add 133 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW 9,7 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum

Predicted reactivity Catalpa bungei, Phaseolus vulgaris, Oryza sativa, Populus balsamifera, Zea mays,Vitis vinifera
Species of your interest not listed? Contact us

Not reactive in

Chlamydomonas reinhardtii, Synechococcus sp. PCC7942

Application examples

Reactant: Nicotiana tabacum (Common tobacco)

Application: Western Blotting

Pudmed ID: 28180288

Journal: J Exp Bot

Figure Number: 5A

Published Date: 2017-02-01

First Author: Schöttler, M. A., Thiele, W., et al.

Impact Factor: 6.088

Open Publication

Immunoblot analysis of photosynthetic complex accumulation in wild-type tobacco and the two ?psaI lines grown under low, intermediate, and high-light conditions. Because the accumulation of most tested proteins was highest under high-light conditions, lanes one to three contain samples diluted to 25%, 50%, and a 100% sample of wild-type tobacco grown under high-light conditions, to allow for semi-quantitative determination of changes in protein abundance. Lanes four and five contain the two transplastomic lines grown at 1000 µE m?2 s?1. Lanes six to eight contain wild-type tobacco and the mutants grown at intermediate light intensities, and lanes nine to eleven contain samples grown at low light intensities. For PSII, the accumulation of the essential subunits PsbB (CP43) and PsbD (D2) and the LHCB1 antenna protein were determined, while for the cytochrome b6f complex, the accumulation of the essential redox-active subunits PetA (cytochrome f), PetB (cytochrome b6), and PETC (Rieske FeS protein) was tested. AtpB was probed as an essential subunit of the chloroplast ATP. For PSI, in addition to the three essential plastome-encoded subunits PsaA, PsaB, and PsaC, the accumulation of the nuclear-encoded subunits PSAD, PSAH, PSAK, PSAL, and PSAN and of the four LHCI proteins (LHCA1, LHCA2, LHCA3, LHCA4) was determined. Finally, we examined the accumulation of Ycf4, the chloroplast-encoded PSI-biogenesis factor encoded in the same operon as PsaI, and the nuclear-encoded assembly factor Y3IP1.

Reactant: Arabidopsis thaliana (Thale cress)

Application: Western Blotting

Pudmed ID: 34360890

Journal: Int J Mol Sci

Figure Number: 2A

Published Date: 2021-07-29

First Author: Gollan, P. J., Trotta, A., et al.

Impact Factor: 5.542

Open Publication

Estimation of the efficacy of free and membrane-associated lumen isolation. Western blot detection of FKBP16-1, PsbO1/PsbO2 and PsaN in thylakoid membrane and lumen fractions isolated during lumen preparations. 1—total thylakoids; 2—thylakoid membranes after removal of the free lumen fraction; 3—thylakoid membranes after removal of the membrane-associated lumen fraction; 4—free lumen; 5—membrane-associated lumen. Lanes 1–3 contain 10 µg protein, lanes 4–5 contain 30 µg protein. Arrows indicate protein of interest.

Additional information

Background

Photosystem I (PSI) of chloroplasts is a multisubunit membrane-protein complex that catalyzes the electron transfer from the reduced plastocyanin (or cytochrome c6) in the thylakoid lumen to the oxidized ferredoxin (or flavodoxin) in the chloroplast stroma. PsaN is necessary for docking plastocyanin to the PSI complex. PSI-N is the only subunit located entirely on the lumenal side of PSI.

Product citations

Selected references Hansson et al. (2007). Knock-out of the chloroplast encoded PSI-J Subunit of Photosystem I in Nicotiana tabacum: PSI-J is required for efficient electron transfer and stable accumulation of photosystem I. FEBS J. 274: 1734-1746.

immunogen:	KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana PsaN protein sequence At5g64040
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophikized
Quantity:	200 µg
Reconstitution:	For reconstitution add 133 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
recommended dilution:	1 : 1000 (WB)
Expected \| apparent MW:	9,7 kDa
Confirmed reactivity:	Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum
predicted reactivity:	Catalpa bungei, Phaseolus vulgaris, Oryza sativa, Populus balsamifera, Zea mays,Vitis vinifera Species of your interest not listed? Contact us
not reactive in:	Chlamydomonas reinhardtii, Synechococcus sp. PCC7942
More images:	Reactant: Nicotiana tabacum (Common tobacco) Application: Western Blotting Pudmed ID: 28180288 Journal: J Exp Bot Figure Number: 5A Published Date: 2017-02-01 First Author: Schöttler, M. A., Thiele, W., et al. Impact Factor: 6.088 Open Publication Immunoblot analysis of photosynthetic complex accumulation in wild-type tobacco and the two ?psaI lines grown under low, intermediate, and high-light conditions. Because the accumulation of most tested proteins was highest under high-light conditions, lanes one to three contain samples diluted to 25%, 50%, and a 100% sample of wild-type tobacco grown under high-light conditions, to allow for semi-quantitative determination of changes in protein abundance. Lanes four and five contain the two transplastomic lines grown at 1000 µE m?2 s?1. Lanes six to eight contain wild-type tobacco and the mutants grown at intermediate light intensities, and lanes nine to eleven contain samples grown at low light intensities. For PSII, the accumulation of the essential subunits PsbB (CP43) and PsbD (D2) and the LHCB1 antenna protein were determined, while for the cytochrome b6f complex, the accumulation of the essential redox-active subunits PetA (cytochrome f), PetB (cytochrome b6), and PETC (Rieske FeS protein) was tested. AtpB was probed as an essential subunit of the chloroplast ATP. For PSI, in addition to the three essential plastome-encoded subunits PsaA, PsaB, and PsaC, the accumulation of the nuclear-encoded subunits PSAD, PSAH, PSAK, PSAL, and PSAN and of the four LHCI proteins (LHCA1, LHCA2, LHCA3, LHCA4) was determined. Finally, we examined the accumulation of Ycf4, the chloroplast-encoded PSI-biogenesis factor encoded in the same operon as PsaI, and the nuclear-encoded assembly factor Y3IP1. Reactant: Arabidopsis thaliana (Thale cress) Application: Western Blotting Pudmed ID: 34360890 Journal: Int J Mol Sci Figure Number: 2A Published Date: 2021-07-29 First Author: Gollan, P. J., Trotta, A., et al. Impact Factor: 5.542 Open Publication Estimation of the efficacy of free and membrane-associated lumen isolation. Western blot detection of FKBP16-1, PsbO1/PsbO2 and PsaN in thylakoid membrane and lumen fractions isolated during lumen preparations. 1—total thylakoids; 2—thylakoid membranes after removal of the free lumen fraction; 3—thylakoid membranes after removal of the membrane-associated lumen fraction; 4—free lumen; 5—membrane-associated lumen. Lanes 1–3 contain 10 µg protein, lanes 4–5 contain 30 µg protein. Arrows indicate protein of interest.
background:	Photosystem I (PSI) of chloroplasts is a multisubunit membrane-protein complex that catalyzes the electron transfer from the reduced plastocyanin (or cytochrome c6) in the thylakoid lumen to the oxidized ferredoxin (or flavodoxin) in the chloroplast stroma. PsaN is necessary for docking plastocyanin to the PSI complex. PSI-N is the only subunit located entirely on the lumenal side of PSI.
All references:	Hansson et al. (2007). Knock-out of the chloroplast encoded PSI-J Subunit of Photosystem I in Nicotiana tabacum: PSI-J is required for efficient electron transfer and stable accumulation of photosystem I. FEBS J. 274: 1734-1746.