PsaN | PSI-N subunit of photosystem I
AS06 109 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Product Information
Immunogen
KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana PsaN protein sequence At5g64040
Host
Rabbit
Clonality
Polyclonal
Purity
Affinity purified serum
Format
Lyophilized in PBS pH 7.4
Quantity
200 µg
Reconstitution
For reconstitution add 133 µl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
9.7 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum
Predicted reactivity
Catalpa bungei, Phaseolus vulgaris, Oryza sativa, Populus balsamifera, Zea mays,Vitis vinifera
Not reactive in
Chlamydomonas reinhardtii, Synechococcus sp. PCC7942
Application examples
Additional information
Background
Background
Photosystem I (PSI) of chloroplasts is a multisubunit membrane-protein complex that catalyzes the electron transfer from the reduced plastocyanin (or cytochrome c6) in the thylakoid lumen to the oxidized ferredoxin (or flavodoxin) in the chloroplast stroma. PsaN is necessary for docking plastocyanin to the PSI complex. PSI-N is the only subunit located entirely on the lumenal side of PSI.
Product citations
Selected references
Hansson et al. (2007). Knock-out of the chloroplast encoded PSI-J Subunit of Photosystem I in Nicotiana tabacum: PSI-J is required for efficient electron transfer and stable accumulation of photosystem I. FEBS J. 274: 1734-1746.
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