showForm Goat anti-rabbit IgG (H&L) AP conjugated secondary antibody

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Goat anti-Rabbit IgG (H&L), ALP conjugated

AS09 607 Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)

Goat anti-Rabbit IgG (H&L), ALP conjugated  in the group Secondary Antibodies / Anti-Rabbit / ALP (alkaline phosphatase) at Agrisera AB (Antibodies for research) (AS09 607)

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Product Information

Immunogen

Purified Rabbit IgG, whole molecule.

Host Goat
Clonality Polyclonal
Purity Affinity purified goat IgG
Format Liquid, clear, colorless.
Quantity 1 mg
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 500-1: 8 000 (ELISA), 1 : 500 -1 : 2000 (IHC), 1 : 500-1: 8 000 (WB)

Reactivity

Confirmed reactivity Rabbit IgG heavy and light chains (H&L).
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

Western blot using anti-HSP16.9 antibodies on wheat

24 µg of Triticum aestivum L. whole leaf extract (1), 23 µg of Triticum aestivum L. whole leaf extract 37°С,3h (2), 22 µg of Triticum aestivum L. whole leaf extract, 37°С, 24h (3), 20 µg of Triticum aestivum L. whole leaf extract, 37°С,24h+50°C/1h (4), 17 µg of Triticum aestivum L. whole leaf extract, 37°С,24h+50°C, 3h (5), 23 µg of Triticum aestivum L. whole leaf extract, Control+50°C, 1h (6).

700 µg of total protein from spring wheat Triticum aestivum L. green leaves extracted with write exact buffer components 100 mM Tris HCl (pH=7.4), 1 mM beta-mercaptoethanol, 1 mM PMSF and denatured with 65.2 mM Tris HCl (pH=6.8), 1mM EDTA, 1% SDS, 20% glycerol, 5% beta-mercaptoethanol at 97°C for 5 min and 20 μg of total protein were separated on 12.5 % SDS-PAGE and blotted 2h on nitrocellulose membrane (GE Healthcare) using tank transfer. Blots were blocked with a skimmed milk 3 % in TBS for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation in TBS. The antibody solution was decanted and the blot washed 2 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG, ALP conjugated, AS09 607, from Agrisera) diluted to 1:1000 in a skimmed milk 3 % in TBS for 1h at RT with agitation. The blot was washed 3 times for 5 min in TBS-T at RT with agitation and developed WB. The proteins were detected with 5-bromo-4-chloro-3-indolyl phosphate (Thermo Scientific) and Nitrotetrazolium Blue (Thermo Scientific). Exposure time was 5.20 minutes.

Courtesy of Dr. Olga Borovik, Laboratory of Physiological Genetics Siberian Institute of Plant Physiology and Biochemistry SB RAS Russia

Additional information

Additional information

Antibody has been affinity purified on solid phase rabbit IgG (H&L).

AP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative

Based upon IEP, this antibody binds to:
heavy chains on rabbit IgG
light chains on all rabbit immunoglobulins

No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.

Related products

Related products

AS09 602 | Goat anti-rabbit IgG (H&L), HRP conjugated

AS09 608 | Goat anti-rabbit IgG (H&L), biotin conjugated

Background

Background

Goat anti-rabbit IgG (H&L)  is a secondary antibody conjugated to AP or ALP (Alkaline phosphatase) which binds to all rabbit immunoglobulins in immunological assays.

Product citations

Selected references Szymańska et al. (2019). SNF1-Related Protein Kinases SnRK2.4 and SnRK2.10 Modulate ROS Homeostasis in Plant Response to Salt Stress. Int J Mol Sci. 2019 Jan 2;20(1). pii: E143. doi: 10.3390/ijms20010143.
Rozpądek et al. (2018). Acclimation of the photosynthetic apparatus and alterations in sugar metabolism in response to inoculation with endophytic fungi. Plant Cell Environ. 2018 Dec 5. doi: 10.1111/pce.13485.
Borovik and Grabelnych (2018). Mitochondrial alternative cyanide-resistant oxidase is involved in an increase of heat stress tolerance in spring wheat. J Plant Physiol. 2018 Dec;231:310-317. doi: 10.1016/j.jplph.2018.10.007.
Aswani et al. (2018). Oxidative stress induced in chloroplasts or mitochondria promotes proline accumulation in leaves of pea (Pisum sativum): another example of chloroplast-mitochondria interactions. Protoplasma. 2018 Sep 11. doi: 10.1007/s00709-018-1306-1.
Giovanardi et al. (2018). In pea stipules a functional photosynthetic electron flow occurs despite a reduced dynamicity of LHCII association with photosystems. Biochim Biophys Acta. 2018 May 24. pii: S0005-2728(18)30129-4. doi: 10.1016/j.bbabio.2018.05.013.
Hanschen et al. (2018). Differences in the enzymatic hydrolysis of glucosinolates increase the defense metabolite diversity in 19 Arabidopsis thaliana accessions. Plant Physiol Biochem. 2018 Mar;124:126-135. doi: 10.1016/j.plaphy.2018.01.009.
Krasuska et al. (2015). Switch from heterotrophy to autotrophy of apple cotyledons depends on NO signal. Planta. 2015 Jul 18.

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