Goat anti-Rabbit IgG (H&L), DyLight® 488 conjugated

Name: Goat anti-Rabbit IgG (H&L), DyLight® 488 conjugated
SKU: AS09 633
Price: 206 EUR
Availability: InStock
Rating: 5 (1 reviews)

AS09 633 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

Goat anti-Rabbit IgG (H&L), DyLight® 488 conjugated in the group Secondary Antibodies / Anti-Rabbit / Fluorescent / DyLight® / DyLight® 488 at Agrisera AB (Antibodies for research) (AS09 633)

Goat anti-Rabbit IgG (H&L), DyLight® 488 conjugated

Product Information

Immunogen

Purified Rabbit IgG, whole molecule

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified goat IgG.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Tested applications Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF)

Recommended dilution 1 : 50- 1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC), 1 : 3000 (IF)

Reactivity

Confirmed reactivity Rabbit IgG heavy and light chains (H&L)

Predicted reactivity Rabbit IgG Heavy and Light chains (H&L)

Application examples

Application examples Application example

Seeds of field bean (Vicia faba L. subsp. minor var. Nadwiślański; DANKO Group; Sobiejuchy) were sterilized using sodium hypochlorite (0.3% v/v) and germinated in Petri dishes on wetted filter paper at room temperature. At 4 d after imbibition, dark-grown seedlings with primary roots 25±5 mm long were selected for experiments. During incubations roots were oriented horizontally in a humid chamber and aerated continuously on a rotary water-bath shaker (30 rpm) at 23°C. Immunocytochemical assays were performed according to the method prescribed earlier (Rybaczek and Maszewski 2006). Excised apical parts of roots (1.5 mm long) were fixed for 45 min (18°C) in PBS-buffered 3.7% paraformaldehyde, washed several times with PBS and placed in a citric acid-buffered digestion solution (pH 5.0; 37°C for 45 min) containing 2.5% pectinase (Fluka), 2.5% cellulase (Onozuka R-10; Serva) and 2.5% pectoliase (ICN). After removing the digestion solution, root tips were washed 3 times in PBS, rinsed with distilled water and squashed onto Super Frost Plus glass slides (Menzel-Gläser). Air-dried slides were pretreated with PBS-buffered 5% BSA at 20°C for 50 min and incubated overnight in a humidified atmosphere (4°C) with rabbit antibody raised against TOPO2 (Agrisera), dissolved in PBS containing 1% BSA (at a dilution of 1:500). Following incubation, slides were washed 3 times with PBS and incubated for 1 h (18°C) with secondary goat anti-rabbit IgG DyLight®488 antibody (Agrisera, AS09 633, 1:3000). Nuclear DNA was stained with 4’,6-diamidino-2-phenyl-indole (DAPI, 0.4 μg/ml; Sigma-Aldrich). Following washing with PBS, slides were air dried and embedded in Vectashield Mounting Media for Fluorescence (Vector Laboratories). Observations were made using Optiphot-2 fluorescence microscope (Nikon) equipped with B-2A filter (blue light; λ ≈ 495 nm) for DyLight-conjugated antibodies and UV-2A filter (UV light; λ ≈ 365 nm) for DAPI. All images were recorded at exactly the same time of integration using DXM 1200 CCD camera.

Courtesy Dr. Dorota Rybaczek, Lodz University, Poland

Reactant: Arabidopsis thaliana (Thale cress)

Application: Immunohistochemistry-immunofluorescence

Pudmed ID: 26516341

Journal: Plant Methods

Figure Number: 7A

Published Date: 2015-10-31

First Author: Pasternak, T., Tietz, O., et al.

Impact Factor: 5.139

Open Publication

3D reconstruction of Arabidopsis root epidermis cells undergoing telophase: co-localization of ?-Tubulin (TUB) and PIN1 in division plates. Four days old Arabidopsis seedlings were fixed for 30 min in formaldehyde. a Anti-PIN1 mouse monoclonal primary antibody (10A7) diluted 1:50 plus ALEXA Fluor ® 555 anti-mouse as secondary antibody diluted 1:800 (shown in green color) and anti-TUB (AS10 681) rabbit primary antibody diluted 1:600 plus Goat anti-rabbit IgG (H&L), DyLight® 488 Conjugate (AS09 633) diluted in 1:3000 as secondary antibody (shown in red color) were used. b Anti-PIN2 Guinea pig polyclonal primary antibody (clone A193) dilution 1:800 plus ALEXA Fluor ® 555 anti-Guinea pig as the secondary antibody dilution 1:800 (show in green color) and anti-TUB (Agrisera, AS10 681) rabbit primary antibody diluted 1:600 plus Goat anti-rabbit IgG (H&L), DyLight® 488 Conjugate (AS09 633) as secondary antibody diluted in 1:3000 (shown in red color) were used. Co-staining with DAPI visualizes nucleus (blue). Scale bar 20 µm. The Insertion in a shows an ortho-view of dividing cells

Reactant: Arabidopsis thaliana (Thale cress)

Application: Immunohistochemistry-immunofluorescence

Pudmed ID: 26516341

Journal: Plant Methods

Figure Number: 7b

Published Date: 2015-10-31

First Author: Pasternak, T., Tietz, O., et al.

Impact Factor: 5.139

Open Publication

Reactant: Arabidopsis thaliana (Thale cress)

Application: Immunohistochemistry-immunofluorescence

Pudmed ID: 26516341

Journal: Plant Methods

Figure Number: 8A

Published Date: 2015-10-31

First Author: Pasternak, T., Tietz, O., et al.

Impact Factor: 5.139

Open Publication

Auxin immunolocalisation in Arabidopsis roots. Four days old Arabidopsis seedlings were treated with 1 µM 1-N-Naphthylphthalamic acid (NPA) for 24 h to enhance accumulation of auxin in roots. Seedlings were fixed for 20 min in 4 % EDAC in 1× MTSB, and next 30 min in 4 % EDAC+ 2 % Formaldehyde. Anti-indole 3 acetic acid (IAA) rabbit primary antibody (Agrisera, AS06 193) diluted 1:600 plus Goat anti-rabbit IgG (H&L), DyLight® 549 Conjugate (AS09 633) as secondary antibody diluted in 1:3000 (shown in red color) were used. Scale bar 20 µm

Additional information

Concentration: 1.0mg/ml

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

DyLight® 488 has a maximum absorbance at 493 nm; Emax = 518 nm.

Based in immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.

No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.

Purity of this antibody is > 95% based on SDS-PAGE.

Background

Goat anti-rabbit IgG, DyLight® 488 Conjugate is a secondary antibody conjugated to DyLight® 488, which binds to all rabbit IgGs in immunological assays.

DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Product citations

Selected references Kucko et al. (2022) The acceleration of yellow lupine flower abscission by jasmonates is accompanied by lipid-related events in abscission zone cells, Plant Science, Volume 316, 2022,111173, ISSN 0168-9452, https://doi.org/10.1016/j.plantsci.2021.111173.
Namyslov et al. (2020). Exodermis and Endodermis Respond to Nutrient Deficiency in Nutrient-Specific and Localized Manner. Plants (Basel). 2020 Feb 6;9(2). pii: E201. doi: 10.3390/plants9020201. (immunolocalization)
Fizesan et al. (2018). Responsiveness assessment of a 3D tetra-culture alveolar model exposed to diesel exhaust particulate matter. Toxicol In Vitro. 2018 Aug 3;53:67-79. doi: 10.1016/j.tiv.2018.07.019.
Liu et al. (2016). Fold formation at the compartment boundary of Drosophila wing requires Yki signaling to suppress JNK dependent apoptosis. Sci Rep. 2016 Nov 29;6:38003. doi: 10.1038/srep38003.
Wang et al. (2016). Complementary expression of optomotor-blind and the Iroquois complex promotes fold formation to separate wing notum and hinge territories. Dev Biol. 2016 Aug 1;416(1):225-34. doi: 10.1016/j.ydbio.2016.05.020. Epub 2016 May 19
Kaulmann et al. (2016). Inflammation related responses of intestinal cells to plum and cabbage digesta with differential carotenoid and polyphenol profiles following simulated gastro-intestinal digestion. Mol Nutr Food Res. 2016 Mar 18. doi: 10.1002/mnfr.201500947.
Jimenez-Lopez et al. (2015). Biogenesis of protein bodies during legumin accumulation in developing olive (Olea europaea L.) seed. Protoplasma. 2015 May 21.

Confirmed reactivity:	Rabbit IgG heavy and light chains (H&L)
predicted reactivity:	Rabbit IgG Heavy and Light chains (H&L)
additional information:	Concentration: 1.0mg/ml Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative. DyLight® 488 has a maximum absorbance at 493 nm; Emax = 518 nm.
additional information (application):	Based in immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins. No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis. Purity of this antibody is > 95% based on SDS-PAGE.
background:	Goat anti-rabbit IgG, DyLight® 488 Conjugate is a secondary antibody conjugated to DyLight® 488, which binds to all rabbit IgGs in immunological assays. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
More images:	Reactant: Arabidopsis thaliana (Thale cress) Application: Immunohistochemistry-immunofluorescence Pudmed ID: 26516341 Journal: Plant Methods Figure Number: 7A Published Date: 2015-10-31 First Author: Pasternak, T., Tietz, O., et al. Impact Factor: 5.139 Open Publication 3D reconstruction of Arabidopsis root epidermis cells undergoing telophase: co-localization of ?-Tubulin (TUB) and PIN1 in division plates. Four days old Arabidopsis seedlings were fixed for 30 min in formaldehyde. a Anti-PIN1 mouse monoclonal primary antibody (10A7) diluted 1:50 plus ALEXA Fluor ® 555 anti-mouse as secondary antibody diluted 1:800 (shown in green color) and anti-TUB (AS10 681) rabbit primary antibody diluted 1:600 plus Goat anti-rabbit IgG (H&L), DyLight® 488 Conjugate (AS09 633) diluted in 1:3000 as secondary antibody (shown in red color) were used. b Anti-PIN2 Guinea pig polyclonal primary antibody (clone A193) dilution 1:800 plus ALEXA Fluor ® 555 anti-Guinea pig as the secondary antibody dilution 1:800 (show in green color) and anti-TUB (Agrisera, AS10 681) rabbit primary antibody diluted 1:600 plus Goat anti-rabbit IgG (H&L), DyLight® 488 Conjugate (AS09 633) as secondary antibody diluted in 1:3000 (shown in red color) were used. Co-staining with DAPI visualizes nucleus (blue). Scale bar 20 µm. The Insertion in a shows an ortho-view of dividing cells Reactant: Arabidopsis thaliana (Thale cress) Application: Immunohistochemistry-immunofluorescence Pudmed ID: 26516341 Journal: Plant Methods Figure Number: 7b Published Date: 2015-10-31 First Author: Pasternak, T., Tietz, O., et al. Impact Factor: 5.139 Open Publication 3D reconstruction of Arabidopsis root epidermis cells undergoing telophase: co-localization of ?-Tubulin (TUB) and PIN1 in division plates. Four days old Arabidopsis seedlings were fixed for 30 min in formaldehyde. a Anti-PIN1 mouse monoclonal primary antibody (10A7) diluted 1:50 plus ALEXA Fluor ® 555 anti-mouse as secondary antibody diluted 1:800 (shown in green color) and anti-TUB (AS10 681) rabbit primary antibody diluted 1:600 plus Goat anti-rabbit IgG (H&L), DyLight® 488 Conjugate (AS09 633) diluted in 1:3000 as secondary antibody (shown in red color) were used. b Anti-PIN2 Guinea pig polyclonal primary antibody (clone A193) dilution 1:800 plus ALEXA Fluor ® 555 anti-Guinea pig as the secondary antibody dilution 1:800 (show in green color) and anti-TUB (Agrisera, AS10 681) rabbit primary antibody diluted 1:600 plus Goat anti-rabbit IgG (H&L), DyLight® 488 Conjugate (AS09 633) as secondary antibody diluted in 1:3000 (shown in red color) were used. Co-staining with DAPI visualizes nucleus (blue). Scale bar 20 µm. The Insertion in a shows an ortho-view of dividing cells Reactant: Arabidopsis thaliana (Thale cress) Application: Immunohistochemistry-immunofluorescence Pudmed ID: 26516341 Journal: Plant Methods Figure Number: 8A Published Date: 2015-10-31 First Author: Pasternak, T., Tietz, O., et al. Impact Factor: 5.139 Open Publication Auxin immunolocalisation in Arabidopsis roots. Four days old Arabidopsis seedlings were treated with 1 µM 1-N-Naphthylphthalamic acid (NPA) for 24 h to enhance accumulation of auxin in roots. Seedlings were fixed for 20 min in 4 % EDAC in 1× MTSB, and next 30 min in 4 % EDAC+ 2 % Formaldehyde. Anti-indole 3 acetic acid (IAA) rabbit primary antibody (Agrisera, AS06 193) diluted 1:600 plus Goat anti-rabbit IgG (H&L), DyLight® 549 Conjugate (AS09 633) as secondary antibody diluted in 1:3000 (shown in red color) were used. Scale bar 20 µm
Picture (footer):	Application example Seeds of field bean (Vicia faba L. subsp. minor var. Nadwiślański; DANKO Group; Sobiejuchy) were sterilized using sodium hypochlorite (0.3% v/v) and germinated in Petri dishes on wetted filter paper at room temperature. At 4 d after imbibition, dark-grown seedlings with primary roots 25±5 mm long were selected for experiments. During incubations roots were oriented horizontally in a humid chamber and aerated continuously on a rotary water-bath shaker (30 rpm) at 23°C. Immunocytochemical assays were performed according to the method prescribed earlier (Rybaczek and Maszewski 2006). Excised apical parts of roots (1.5 mm long) were fixed for 45 min (18°C) in PBS-buffered 3.7% paraformaldehyde, washed several times with PBS and placed in a citric acid-buffered digestion solution (pH 5.0; 37°C for 45 min) containing 2.5% pectinase (Fluka), 2.5% cellulase (Onozuka R-10; Serva) and 2.5% pectoliase (ICN). After removing the digestion solution, root tips were washed 3 times in PBS, rinsed with distilled water and squashed onto Super Frost Plus glass slides (Menzel-Gläser). Air-dried slides were pretreated with PBS-buffered 5% BSA at 20°C for 50 min and incubated overnight in a humidified atmosphere (4°C) with rabbit antibody raised against TOPO2 (Agrisera), dissolved in PBS containing 1% BSA (at a dilution of 1:500). Following incubation, slides were washed 3 times with PBS and incubated for 1 h (18°C) with secondary goat anti-rabbit IgG DyLight®488 antibody (Agrisera, AS09 633, 1:3000). Nuclear DNA was stained with 4’,6-diamidino-2-phenyl-indole (DAPI, 0.4 μg/ml; Sigma-Aldrich). Following washing with PBS, slides were air dried and embedded in Vectashield Mounting Media for Fluorescence (Vector Laboratories). Observations were made using Optiphot-2 fluorescence microscope (Nikon) equipped with B-2A filter (blue light; λ ≈ 495 nm) for DyLight-conjugated antibodies and UV-2A filter (UV light; λ ≈ 365 nm) for DAPI. All images were recorded at exactly the same time of integration using DXM 1200 CCD camera. Courtesy Dr. Dorota Rybaczek, Lodz University, Poland
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Goat
immunogen:	Purified Rabbit IgG, whole molecule
Purity:	Immunogen affinity purified goat IgG.
Quantity:	1 mg
recommended dilution:	1 : 50- 1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC), 1 : 3000 (IF)
Reconstitution:	For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
storage:	Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
tested applications:	Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF)
All references:	Kucko et al. (2022) The acceleration of yellow lupine flower abscission by jasmonates is accompanied by lipid-related events in abscission zone cells, Plant Science, Volume 316, 2022,111173, ISSN 0168-9452, https://doi.org/10.1016/j.plantsci.2021.111173. Namyslov et al. (2020). Exodermis and Endodermis Respond to Nutrient Deficiency in Nutrient-Specific and Localized Manner. Plants (Basel). 2020 Feb 6;9(2). pii: E201. doi: 10.3390/plants9020201. (immunolocalization) Fizesan et al. (2018). Responsiveness assessment of a 3D tetra-culture alveolar model exposed to diesel exhaust particulate matter. Toxicol In Vitro. 2018 Aug 3;53:67-79. doi: 10.1016/j.tiv.2018.07.019. Liu et al. (2016). Fold formation at the compartment boundary of Drosophila wing requires Yki signaling to suppress JNK dependent apoptosis. Sci Rep. 2016 Nov 29;6:38003. doi: 10.1038/srep38003. Wang et al. (2016). Complementary expression of optomotor-blind and the Iroquois complex promotes fold formation to separate wing notum and hinge territories. Dev Biol. 2016 Aug 1;416(1):225-34. doi: 10.1016/j.ydbio.2016.05.020. Epub 2016 May 19 Kaulmann et al. (2016). Inflammation related responses of intestinal cells to plum and cabbage digesta with differential carotenoid and polyphenol profiles following simulated gastro-intestinal digestion. Mol Nutr Food Res. 2016 Mar 18. doi: 10.1002/mnfr.201500947. Jimenez-Lopez et al. (2015). Biogenesis of protein bodies during legumin accumulation in developing olive (Olea europaea L.) seed. Protoplasma. 2015 May 21.

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