Agrisera blog

Two conferences supported this week by Agrisera

International Symposium on Iron Nutrition and Interactions in Plants (ISINIP) took place between 8th and 12th of July in Düsseldorf in Germany. Scientific sessions covered the following topics:

  • Crop iron physiology 
  • Micronutrient biofortification 
  • Plant-soil-microbe interactions 
  • Alkaline and acidic soil environments 
  • Transcription factor networks 
  • Iron acquisition and transport 
  • Whole-plant iron physiology and regulation 
  • Iron physiology and interaction with other elements 

Best Poster Prizes from Agrisera were awarded to two young scientists. 

Plant Epigenetics meeting took place between 10th and 12th of July in Clermond-Ferrand in France. Agrisera supported this meeting with hard copies of educational posters focused on epigenetics, which were developed in collaboration with scientists in the field. 
 

 
 

 

Two conferences supported by Agrisera this week

Read more 2024-07-13

Do not miss: 15 % off all antibodies to rice proteins

Rice is a staple food for more than half of the world's population, particularly in Asia, Africa, and Latin America. Research on this important crop includes: 
-Nutritional Improvement like Golden Rice with increased vitamin A.
-Investigation of rice climate change adaptation by development of varieties which are more tolerant to drought, salinity, and temperature extremes as well as flooding.
-Pest and disease, which will help to reduce use of chemicals and lead to more sustainable agriculture.
-Understanding of genetic diversity and preservation of wild rice species, which are valuable genetic resources for future breeding programs.

As research on rice is vital for ensuring food security between July and end of September, all Agrisera antibodies to rice proteins are offered with a 15 % discount. Browse Agrisera rice antibody collection here.
 

 

Agrisera antibodies to rice proteins 15 % off

Read more 2024-07-04

Agrisera supported Second European Congress on Photosynthesis

Second European Congress on Photosynthesis (EPS2) took place between 25th-28th of June in Padova, Italy.

Agrisera supported the meeting with hard copies of educational posters focused on photosynthesis, which were available to participants. 
Agrisera Educational Posters were developed by Dr. Dmitry Shevela, Prof. Govindjee, and other leading scientists in the covered fields. More information on the authors can be found below. Graphic design by Dmitry Shevela (SciGrafik).
 

 

EPS2 Padova, Italy  2024

Read more 2024-06-29

Do not miss: 15 % off all epitope tag antibodies until end of 2024

Epitope tag antibodies offer several benefits that make them invaluable tools for studying proteins in a wide range of applications like: Western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, and flow cytometry.

Most commonly used epitope tags are:
HIS (3X or 6X), FLAG, HA, Myc, GFP and its variants. Agrisera offers antibodies to a wide range of common and less commonly used epitope tags.

HIS | FLAG | HA | Myc | GFP
 
From 1st of July until end of December, all Agrisera epitope tag antibodies are going to be provided with a 15 % discount.

Promotion code to use is: Tag15.
 

 

Agrisera epitope tag antibodies 15 % off
To browse all Agrisera epitope tag antibodies, check here.

Read more 2024-06-28

Agrisera at Plant Biology 2024, in Honolulu, Hawaii

Agrisera attended the Plant Biology 2024 conference in Honolulu, HI, USA, held between the 22nd and 26th of June. Agrisera was represented by Edith Kalén and Chris Brown.

Thank you to everyone who visited Agrisera's booth to discuss antibodies and plant science, and to the conference organizers for yet another successful meeting. Our warmest congratulations to ASPB on their 100th anniversary!

Agrisera's Educational Posters were available free of charge at the conference. In case you did not get a chance to obtain copies, you can request them, free of charge, here.

Hope to see you in Milwaukee next year!

 


Top: Agrisera's booth at Plant Biology 2024
Bottom, left to right: Conference opening reception, ASPB's 100th anniversary party.

Read more 2024-06-27

Confirm fraction purity with Agrisera compartment marker antibodies

Nguyen et al. used Agrisera antibodies to α–phosphoenolpyruvate carboxylase (PEPC) and histone 3 (H3) in the recent publication "The processed C-terminus of AvrRps4 effector suppresses plant immunity via targeting multiple WRKYs ", published in Journal of Integrative Plant Biology, June 2024.

The following antibodies were used to confirm the cytoplasmic and nuclear fraction's purity:

Anti-PEPC (AS09 458) (cytoplasmic fraction)

Anti-H3 (AS10 710) (nucelar fraction)



 



    Agrisera antibodies used to confirm fraction purity

Read more 2024-06-22

Meet with Agrisera at Plant Biology 2024 in Honolulu


Between June 22nd - 26th, Agrisera is going to be present at the Plant Biology 2024, Honolulu, Hawai. The conference is organized each year by American Society of Plant Biologists (ASPB). The theme of this year's meeting is: "Celebrating Our Past through Science for Our Future".

Sc.Eng. Edith Björnberg Kalén and Dr. Chris Brown are representing Agrisera at the meeting. You are welcome to come by Agrisera booth and talk antibodies with them.  Dr. Brown has been involved in the development of Agrisera's Global Antibodies, to a wide range of key photosynthetic proteins, with broad species reactivity. 

Participants can pick up free hard copies of Agrisera's Educational Posters, prepared in collaboration with scientists in the field of photosynthesis and epigenetics, as well as the Agrisera product catalog and Agrisera Western blot troubleshooting guide

 

    Plant Biology 2024

Come by and meet with Agrisera!
 

Read more 2024-06-20

Multiple Agrisera photosynthesis antibodies applied for a novel mutant characterization

Krysiak et al. used several of Agrisera antibodies to photosynthetic proteins in the recent publication "Light-independent pathway of STN7 kinase activation under low temperature stress in runner bean (Phaseolus coccineus L.)", published in BMC Plant Biology, June 2024.

The following antibodies were used in mutant characterization:

Anti-AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit antibodies), AS05 085
Anti-CURT1A | Curvature thylakoid 1A, AS08 316
Anti-Lhca2 | PSI type II chloropyll a/b-binding protein, AS01 006
Anti-Lhcb1 | LHCII type I chlorophyll a/b-binding protein, AS01 004
Anti-Lhcb1-P | LHCII type I chlorophyll a/b-binding protein, phopshorylated, AS13 2704
Anti-Lhcb2 | LHCII type II chlorophyll a/b-binding protein, AS01 003
Anti-Lhcb2-P | LHCII type II chlorophyll a/b-binding protein, phosphorylated, AS13 2705
Anti-NtrC | Thioredoxin reductase (TR/TRxR) (higher plants), AS07 243
Anti-PsaA | PSI-A core protein of photosystem I, AS06 172
Anti-PsaB | PSI-B core subunit of photosystem I, AS10 695
Anti-PsbA | D1 protein of PSII, DE-loop, AS10 704
Anti-PsbC | CP43 protein of PSII, AS11 1787
Anti-RbcL| Rubisco large subunit, form I (rabbit), AS03 037



 



   Mutant characterization using Agrisera antibodies

Read more 2024-06-16

Agrisera antibodies to photosynthetic proteins support research on how to improve crop yields

Zhao et al. used several of Agrisera antibodies to photosynthetic proteins in the recent publication "Psb28 protein is indispensable for stable accumulation of PSII core complexes in Arabidopsis",  published in Plant Journal, May 2024.

The following antibodies were used in mutant characterization:

Anti-AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial), AS05 085
Anti-Cyt f | Cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex, AS08 306 (AS20 4377)
Anti-Deg8 | protease Do-like 8 (chloroplastic), AS14 2767
Anti-FtsH1+FtsH5 | ATP-dependent zinc metalloprotease FtsH1+FtsH5 (chloroplastic), AS16 3930
Anti-FtsH2+FtsH8 | ATP-dependent zinc metalloprotease FtsH2+FtsH8 (chloroplastic), AS16 3929
Anti-Lhcb2 | LHCII type II chlorophyll a/b-binding protein, AS01 003
Anti-Lhca2 | PSI type II chloropyll a/b-binding protein, AS01 006
Anti-PsaA | PSI-A core protein of photosystem I, AS06 172
Anti-PsbA | D1 protein of PSII, C-terminal, AS05 084
Anti-PsbB | CP47 protein of PSII, AS04 038
Anti-PsbC | CP43 protein of PSII, AS11 1787
Anti-PsbD | D2 protein of PSII, AS06 146
Anti-PsbE | Alfa subunit of Cytochrome b559 of PSII, AS06 112
Anti-PsbF | beta subunit of Cytochrome b559 of PSII, AS06 113
Anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-peptide), AS05 092
Anti-RbcL | Rubisco large subunit, form I, AS03 037





    Agrisera antibodies upport research on photosynthesis

Read more 2024-06-08

Agrisera supports two conferences this week

This week, Agrisera supported two conferences:

International Society of Root Research, 12th International Symposium, June 2nd-7th, Leipzig, Germany. The three most important global challenges in terms of root biology are:  climate change, biodiversity loss, and food security. Root research has a fundamental role in helping to solve these problems.

Postdoc Day 2024, 6th of June, UNIGE, Switzerland is organized each year by UNIGE PostDocs. Agrisera supported the meeting with The Best Talk Prize and the Agrisera Educational Posters


    Agrisera plant conference support June 2024

Read more 2024-06-07

Meet with Agrisera at 2nd American Photosynthesis Congress


Agrisera is present at the 2nd American Photosynthesis Congress, held June 3rd-6th, at Georgia State University, USA. The conference covers all aspects of photosynthesis research. 

Participants can pick up free hard copies of Agrisera's Educational Posters, prepared in collaboration with scientists in the field of photosynthesis, as well as the Agrisera product catalog and Agrisera Western blot troubleshooting guide

Dr. Chris Brown is representing Agrisera at the congress. Come by and ask him any questions about antibodies for photosynthesis research. Dr. Brown has been involved in the development of Agrisera's Global Antibodies, to a wide range of key photosynthetic proteins, with broad species reactivity.

   Agrisera at 2nd American Photosynthesis Congress
Dr. Chris Brown at Agrisera's booth.

Read more 2024-06-04

Agrisera supports 13th International Coference for Plant Mitochondrial Biology


Agrisera supported the 13th International Coference for Plant Mitochondrial Biology (ICPMB), held May 26th-30th, in St Malo, France. This biennial meeting is an international forum for junior and senior academics researchers worldwide, who specialize in various aspects of plant mitochondrial biology.

Participants could pick up free hard copies of Agrisera's Educational Posters, prepared in collaboration with scientists in the covered fields, as well as the Agrisera product catalog and Agrisera Western blot troubleshooting guide



   Free image of plant mitochondria

Read more 2024-05-27

Study chloroplast biogenesis and architecture with Agrisera antibodies


Chloroplasts are essential plant cell organelles, responsible for photosynthesis and other important metabolic processes. Their biogenesis and architecture are complex and highly regulated processes. 

Agrisera antibodies can help you investigate various proteins involved in this process.
The antibody collection includes: 

CURT1A | Curvature thylakoid 1A
CURT1B | Curvature thylakoid 1B
CURT1C | Curvature thylakoid 1C
CURT1D | Curvature thylakoid 1D


You are always welcome to provide us new antibody suggestions and be the first to use the produced antibody.



  Free chloroplast image

Read more 2024-05-24

Investigate proteins involved in sucrose metabolism with Agrisera antibodies

Sucrose metabolism plays a crucial role in energy production, carbon partitioning, and sugar transport. It is therefore essential for cellular function, growth and development in plants.

Using Agrisera antibodies, proteins involved in various stages of this process can be tracked. 

Sucrose breakdown involves an enzyme called sucrase (invertase), which cleaves sucrose into the monosaccharides glucose and fructose.

Sucrose biosynthesis occurs primarily in the cytoplasm of photosynthetic cells, and involves the enzymes sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP). These enzymes are involved in the production of sucrose, which can then be transported to other plant tissues for storage or energy utilization.


Check out Agrisera's full collection of antibodies to proteins involved in sucrose metabolism

 



  Free image of maize in vegetative stage

Read more 2024-05-19

Track changes in LHC proteins using most published anti-LHC antibodies

Light-harvesting complexes (LHCs) are essential components of the photosynthetic machinery, allowing photosynthetic organisms to efficiently capture and utilize light energy for the synthesis of organic molecules. The LHCs are integral membrane protein complexes found in the thylakoid membranes of plants, algae, and cyanobacteria. 

Agrisera's LHC antibody collection has been available since the year 2001, with hundreds of scientific references for a wide range of plant and algal species. 
 
Check out Agrisera's LHC antibodies: 
Lhca1 | Lhca2 | Lhca3 | Lhca4 | Lhca5 | Lhcb1 | Lhcb2 | Lhcb3 | Lhcb4 | Lhcb5 | Lhcb6 

Full collection: Antibodies Plant/AlgalPhotosynthesisLHC
    Free image of a chloroplast

Read more 2024-05-12

Agrisera supports BioLock student conference at the University of Wrocław

 



Agrisera supports BioLock student conference, held between the 10th and 12th of May at the University of Wrocław, Poland. The aim of the meeting is, as the organizers describe it, "to expand horizons in the fields of biotechnology, biochemistry, and related disciplines. In other words, embracing both the most crucial and the latest scientific achievements!"


Agrisera supported the meeting with free hard copies of our Educational Posters.



   BioLock Student Conference

Read more 2024-05-10

Secondary Antibody Guide

Finding the correct secondary antibody can be difficult, so we have put together a Secondary Antibody Guide to help you find exactly what you need. A lot of what you need to know about Agrisera secondary antibodies can be found in their name, but it may be hard to decipher. Our guide walks you through common terms related to secondary antibodies, as well as antibody recommendations and FAQs.

Check out our guide and find answers to questions like:
  • Which secondary antibody to choose for Western blot for protein detection in plant and algal samples?
  • What is the Laser lane, Common filter set, Excitation max and Emission max for fluorescent dyes?
  • What are F(ab)'2 and Fc fragments?
  • What is the meaning of Pre-adsorbed?
And much more...

Check out Agrisera's Secondary Antibody Guide!
 

 Antibody chains
Light spectrum Dylight
Fluorescent dye table

Check out our guide for information on antibody structure, fluorescent dyes and much more!


Read more 2024-05-06

Shorten the time of Western blot or IL by using directly conjugated primary antibodies

To directly conjugated a primary antibody to an enzyme, like ALP or HRP, or a fluorescent dye, like DyLight®, the antibody must be of the highest purity; purified against the specific antigen. 

Q: What are the benefits of using directly conjugated primary antibodies?

A: The time necessary to complete all steps of a Western blot or immunolocalization protocol, is shortened considerably, as there is no need for secondary antibody incubation and subsequent washes, before the final reaction can be visualized. Potential cross-reactions, which may occur when using secondary antibodies, are also eliminated. Possible sample loss due to washing steps is also reduced. 

Until end of 2024, all directly conjugated Agrisera primary antibodies are discounted by 10%, when applying the discount code Primconj10.

Check out the complete Agrisera collection of directly conjugated primary antibodies here


  Directly conjugated primary antibodies
Using directly conjugated primary antibodies, is most beneficial for protein targets of high and moderate abundance in the analyzed tissue.

Read more 2024-05-03

20 % off for antibodies to proteins involved in electron transfer in photosynthesis

Electron transfer in the thylakoid membrane generates energy through the synthesis of adenosine triphosphate (ATP). The enzyme ATP synthase catalyzes the formation of ATP from adenosine diphosphate (ADP) and inorganic phosphate (Pi), using the proton gradient established across the membrane. Additionally, NADPH, produced during electron transfer, serves as a source of reducing power for the Calvin cycle, where carbon dioxide is fixed and converted into carbohydrates. This process in the thylakoid membrane plays a crucial role in harnessing light energy and converting it into chemical energy.

Agrisera's collection of antibodies to proteins involved in electron transfer offers you the following advantages:
  • Can be used at high dilutions
  • Broad species reactivity, including higher plants, mosses, algae, cyanobacteria and diatoms
  • Suitable for Western blot
  • Cited in hundreds of publications, over the last 20 years

Explore and pick up suitable antibodies here.
 Free scheme of thylakoid membrane
For more images of schemes and photosynthetic complexes, check free images for download here

Read more 2024-04-26

Get new tips and tricks for Western blot during Agrisera workshop on 16th of May at 11 am CET

Agrisera online Wstern blot workshop

Conducted by: Dr. Joanna Porankiewicz Asplund, Technical Support Manager from the Swedish company Agrisera
Duration: Approximately 2 hours, with Q&A sessions, and a break, during which you have the chance to win prizes in an online quiz. Western blot and antibody questions can be sent in advance to [email protected] 
Technique: Western blot is used in laboratories working with protein detection in complex mixes, as well as protein quantification.
Suitable for: Beginners and experienced users of this technique, working various types of samples, including bacterial extracts, plant tissue and algal cell cultures.
About the presenter: Dr. Joanna Porankiewicz Asplund, Technical Support Manager at Agrisera, has been working with antibody validation for over 20 years, and has troubleshooted hundreds of blots, which has allowed her to gain extensive experience, and an overview on potential difficulties and ways to address them. She is responsible for technical support at Agrisera, and will be happy to share her knowledge during the workshop.
Provided materials:
• Certificate of participation
• Slides of the presentation in pdf format, with useful links
• Slides of the presentation in ppt format, for free use in teaching
• Recording of the workshop

Sign up for this free workshop by sending an email to [email protected]!
Start: 11 am (Central European Time), 10 am (UK), 12 am (Helsinki)

Feedback from participants of previous workshops: 

"Thanks! It was a very nice and informative workshop :)" – Wageningen University & Research Laboratory of Biochemistry, The Netherlands.

"Thank you very much for the workshop! I myself learned quite some additional things, I didn’t know before. And I talked to some of my colleagues just now and they also really liked your workshop. Thanks also for providing the pdf, I will send them to the participants! I really enjoyed your presentation and I can see how dedicated you are about western blots, it was really great to listen to you!" – Max Planck Institute of Molecular Plant Physiology in Potsdam-Golm, Germany. Read more feedback here 

Read more 2024-04-25

Detect and quantify Rubisco using antibodies with 20 years of publication record

Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous, from purple bacteria to flowering plants, and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.

There are the following advantages of using Agrisera antibodies to Rubisco:
  • Working in very high dilutions, 1: 10 000 1h/RT incubation
  • Broad species reactivity, including higher plants, mosses, algae, cyanobacteria and diatoms. One and the same antibody will detect and quantify Rubisco in thousands of species
  • Suitable for Western blot, immunolocalization and tissue printing
  • Possibility of quantification in quantitative Western blot and ELISA
  • Cited in hundreds of publications, over the last 20 years

In the recent publication by Nuamzanei et al. (2024). "Impact of polyvinyl chloride (PVC) microplastic on growth, photosynthesis and nutrient uptake of Solanum lycopersicum L. (Tomato)", was investigated, using anti-RbcL antibodies from Agrisera. 

Explore and pick up suitable antibodies or kits for the detection of the most abundant protein on Earth, Rubisco.
  Rubisco from I

For images of Rubisco, and schemes in which this protein is involved, check out our free images for download here

Read more 2024-04-19

Which secondary antibody to choose for Western blot for protein detection in plant and algal samples?

A matching secondary antibody, that can be used at high dilutions, will contribute to good Western blot results, with less risk for background signal. Beyond this, it can be used for many experiments, and is therefore very cost-efficient. The differences between secondary antibodies from various suppliers are discussed here.

Agrisera's primary antibodies are produced in chicken, goat, mouse, rabbit and rat. The following high-titer secondary antibodies are appropriate matches: 
At the bottom of each primary antibody product page, you will find a recommended secondary antibody to use with it. 

*This antibody is in the group of the most published antibodies in the world, based on publication record analyses performed by CiteAB.


  Agrisera secondary antibody

If you need advice on the choice of the most suitable secondary antibody for your experiments, please ask us, either through the chat on Agrisera website, or by email.

Read more 2024-04-17

Study Photosystem I with Agrisera comprehensive antibody set

Over the last 20 years, Agrisera has developed antibodies to study Photosystem I (PSI). This protein complex plays a central role in the conversion of light energy into chemical energy, in the form of ATP and NADPH. This energy is then used to power the biochemical reactions of photosynthesis, and ultimately produce carbohydrates. Agrisera antibodies specifically targeting PSI components can help accurately identify and quantify these proteins in plant, algal and diatom samples. This enables comparative studies, and facilitates the understanding of photosynthetic processes across a wide taxonomic range.

Agrisera PSI antibodies can be applied in:

Western blotting to detect the presence of PSI subunits in plant, algae and diatom samples, which will help in understanding the expression levels of PSI proteins under different experimental conditions.

• Immunofluorescence and Immunohistochemistry to visualize the spatial distribution of PSI within plant and algal cells to study the subcellular localization of PSI and its dynamics under various physiological conditions.

• Co-immunoprecipitation (Co-IP) to identify protein-protein interactions involving PSI subunits or associated proteins. to determine the molecular interactions and protein complexes involved in PSI function and regulation.

Agrisera PSI antibodies will help determine the impact of genetic mutations on PSI function and assembly.

Browse the full collection
    Free image of photosystem I

Agrisera PSI antibodies serve as valuable tools for investigating various aspects of PSI structure, function, and regulation in photosynthetic organisms. You can rely on the strong publication record for these antibodies.

Choose a set of PSI antibodies for your studies: 

PsaB | PsaD | PsaC | PsaEPsaA | PsaK | PsaN | PsaL | PsaF | PsaH | PsaG | PsaO

Read more 2024-04-14

10% discount in return for feedback

Agrisera offers a 10% discount on any product from our catalog, in return for a short note left on the specific product page. 

Example of product reviews:

Product reviews


To leave a review:
  • Go to the product page for an Agrisera antibody or reagent you have previously used
  • Find the tab "Reviews"
  • Click on "Leave a review"
  • Write your short comment, including details like: type of extract, technique, dilution and overall result obtained with this product
  • Save your comment

Once the review is submitted, contact us at [email protected] and you will be granted a 10% discount on your next order. 

Thank you!


Read more 2024-04-12

Agrisera supports Eastern Regional Photosynthesis Conference 2024

Agrisera supports the Eastern Regional Photosynthesis Conference 2024, held between 12th and 14th of April, at The Marine Biological Laboratory (MBL) in Woods Hole, MA, USA.

The conference program will includes the following topics:

• Mechanism of Light Harvesting and Regulation
• Light Driven Reactions
• Carbon Fixation and Concentration
• Evolution and Applications of Photosynthesis

Agrisera Educational Posters
, focused on photosynthesis, will be available as hard copies for the meeting participants. Agrisera will also conduct an online quiz, with the first prize being the book Photosynthesis: Solar Energy for Life, by Dr. Dmitry Shevela, Prof. Lars Olof Björn, Prof. Govindjee.


 
 
   
Free image of PSII
 
The above image, and many other images and schemes by Scigrafik and Agrisera, can be downloaded for free here.

 

Read more 2024-04-12

Most published photosynthetic antibodies, 20 % off until end of June 2024

Agrisera's antibody collection to proteins involved in photosynthesis has been developed over the last 20 years, in close collaboration with researches in the field, from all around the world.

The collection contains antibodies targeting proteins from the following groups: 

ATPsynthase | Carotenoid metabolism | Chaperones/Transporters | Chloroplast biogenesis/architecture | Chlorophyll | Green Cut | LHC | Light acclimation | Kinases | PSI (Photosystem I) | PSII (Photosystem II) | Proteases | Protein standards-quantitation | Rubisco/Carbon metabolism | Sucrose metabolism

Each year, hundreds of publications are published, using these antibodies, which makes them the most used photosynthetic antibodies on the market. Agrisera antibodies place 31st of over 250 catalog antibody suppliers, based on publication record, not limited to plant science.


 
 
   
Photosynthesis leaf

For 20% discount for antibodies to proteins involved in photosynthesis, use promotional code Photo20. Valid until end of June 2024.

Needed antibody not found in our collection? Contact us!

Read more 2024-04-05

Versatile ECL reagent set, which can save your day!

Question: If something goes wrong with the development of a Western blot with ECL, or if I have too high background or too low signal, can I redo the reaction on the same blot?

Answer: Yes! Within the same day. 
Simply wash the membrane with the appropriate washing buffer, used in your protocol, 1-2 times for 5-10 minutes, and apply another one of the ECL reagents from the Agrisera ECL reagent set. If there is too much background, and a very strong band for the target protein, apply Agrisera ECL Bright. If a target protein band is not detected, apply Agrisera ECL SuperBright

If you want to learn more about how to improve your Western blot, click here.
 
 
   Agrisera's versatile ECL set of 2 reagents with different sensitivity

Blot A shows an abundant photosynthetic protein, PsbA, visualized with too sensitive of a reagent, which was washed away, and the membrane was subsequently developed with less sensitive Agrisera ECL Bright (Blot B). 

Read more 2024-03-28

Take advantage of Agrisera antibodies to proteins involved in response to plant pathogen attack

As plants cannot move and change their habitat quickly as animals can, they have instead developed other amazing strategies to cope with various pathogen attacks. Listed below are some examples of plant pathogens.

Fungal diseases: These pathogens typically thrive in warm, moist conditions and can infect various parts of the plant, such as leaves, stems, fruits, and roots.
Bacterial diseases: Bacterial pathogens infect plants through wounds or natural openings, causing diseases like bacterial wilt, leaf spot, and soft rot, and can spread rapidly through the whole plant.
Viral diseases: Viruses can infect plants through vectors such as insects, nematodes, and even human activities, like grafting. Viral diseases can significantly reduce crop yields and quality.
Nematode damage: Nematodes are microscopic roundworms that feed on plant roots, leading to reduced nutrient uptake and stunted growth. Root-knot nematodes, for example, cause the formation of galls on roots, interfering with water and nutrient absorption.

All antibodies to proteins involved in environmental stress response are offered at a 20% discount until the end of March. Use discount code Stress20.
 
 
 Agrisera Antibodies to proteins involved in response to pathogen attack

Read more 2024-03-23

Agrisera supports Jan Andersson Award of the Australian Society of Plant Scientists

Agrisera is sponsoring this year's Jan Anderson Award, granted by the Australian Society of Plant Scientists

Joan (Jan) Mary Anderson was an adjunct professor at the Australian National University. She received an honorary doctorate at Umeå University (Sweden) in 1998.  She was an internationally acclaimed plant biologist, renowned for her discoveries on the organization of the intricate membrane structures that carry out solar energy conversion in cells of green plants. Her work was the first to show that the photosynthetic mechanisms consists of two fundamental components; Photosystem I and Photosystem II. Her research career spanned over 55 years, mostly at the Division of Plant Industry CSIRO Canberra (1961-97), and later at the Research School of Biological Sciences at the Australian National University.

Jan was a passionate and creative female scientist who was well ahead of her time. She was an inspiration to young researchers, for whom her enthusiasm and curiosity were infectious. Professor Jan Anderson died on 28 August 2015 after a short illness. In recognition of her life and achievements in photosynthesis research as a pioneering female scientist, this award will acknowledge talented female plant science researchers. 


 
 
   Australian Society of Plant Scientists

Full description of the award

Read more 2024-03-22

Agrisera supported the 37th Molecular Biology of Plants meeting in Germany

The 37th Molecular Biology of Plants conference, organized by the Section Plant Physiology and Molecular Biology of the Germany Society for Plant Sciences, took place the 4th-7th of March, North Rhine-Westphalia, Germany. The organizers were Prof. Dr. Christopher Grefen (Bochum), Prof. Dr. Ute Höcker (Cologne), and Prof. Dr. Andreas Meyer (Bonn).

Agrisera supported the meeting with The Best Poster Prize, awarded to Dr. Marc Somssich from the Max Planck Institute for Plant Breeding Research in Cologne. The winning poster title was "Spatially resolved immune responses of Arabidopsis thaliana to colonization by Fusarium oxysporum".


Participants could also enjoy hard copies of Agrisera's Educational Posters, covering topics such as photosynthesis and epigenetics, and developed with scientists in these fields.

 
 
  Winner of Agrisera Best Poster Prize 2024

Left to right: Prof. Ute Hoecker and Dr. Marc Somssich. Courtesy of Dr. José Ugalde from the University of Bonn. 

Read more 2024-03-17

Wide species range of blocking serum for ELISA and immunolocalization

For most immunoassays, like immunolocalization, ELISA or Western blot, blocking is a crucial step. This procedure blocks spaces that are not occupied by immobilized proteins, and thus prevents non-specific binding of the antibodies used in the assay. If blocking is not performed, false signals or high background will occur.

If normal serum is used as a blocker, it is recommended to use serum derived from the same host species as the detection antibody, conjugated with a reporter enzyme, like HRP (Horseradish peroxidase) and ALP (Alkaline phosphatase), or a fluorochrome, like DyLight®, FITC or others. 

Example: 
Primary antibody: Rabbit anti-target protein

Blocking serum: Normal Chicken Serum (usually used at 5% v/v in PBS with Tween®20 detergent)

Secondary antibody: Chicken anti-Rabbit IgG-HRP conjugated

Note: Rabbit serum cannot be used for blocking in such a combination.
 
  Agrisera blocking serum
Agrisera blocking serum from various species, is provided in three purity grades: non-purified, purified on Protein G and ultrapure, available as in research and bulk quantities.

Not certain which blocker to use? Ask Agrisera.

Read more 2024-03-15

Plant oxidative stress antibody set, 20 % off until end of March

Oxidative stress response in plants involves a complex network of proteins that work together to mitigate the damage caused by reactive oxygen species (ROS), such as superoxide radicals, hydrogen peroxide, and hydroxyl radicals. Below is a list of the main proteins involved in this process:

  • Superoxide dismutase (SOD): Enzyme that catalyzes the dismutation of superoxide radicals into oxygen and hydrogen peroxide. There are different isoforms of SOD found in various cellular compartments, such as cytosol, mitochondria and chloroplast.
  • Catalase (CAT): Enzyme that catalyzes the decomposition of hydrogen peroxide into water and oxygen. It is primarily located in peroxisomes and helps neutralize excess hydrogen peroxide, generated during oxidative stress.
  • Ascorbate peroxidase (APX): Enzyme that uses ascorbate as a reducing agent to scavenge hydrogen peroxide and convert it into water, while oxidizing ascorbate to monodehydroascorbate. This enzyme is crucial for maintaining the redox balance in chloroplasts and other cellular compartments.
  • Glutathione peroxidase (GPX): Enzyme that utilizes glutathione (GSH) as a cofactor to reduce hydrogen peroxide and lipid hydroperoxides to their corresponding alcohols. It plays a significant role in protecting cells from oxidative damage by ROS.
  • Glutathione S-transferases (GSTs): Family of enzymes that catalyze the conjugation of glutathione to a wide range of electrophilic compounds, like lipid peroxides and xenobiotics. They are involved in both detoxification processes and in the regulation of cellular redox status.

   Plant Oxidative Stress antibody set

Choose 5 specific antibodies, combined according to your requirements from the following antibodies:

APX Cat Cu/ZnSOD | FeSOD | HRP Gamma-ECS | GR | GS | MnSOD | RBOHD

Offered at 20% discount until the end of March, with the promotion code Stress20.

The set also includeds a high-titer secondary Goat anti-Rabbit antibodies, HRP conjugated and Agrisera ECLBright detection reagent. 
Read more 2024-03-08

Online Western blot workshop for Australia and New Zealand with Agrisera distributor, Sapphire Bioscience

Western blot issues?

Get them resolved and learn many new tips on how to improve your results, in an online Western blot workshop, organized by Agrisera and Sapphire Bioscience, Agrisera's distributor in Australia and New Zealand.

Date: Wednesday, March 20th, 3-5 PM (AEDT). 
Request the link and send your questions here.

Each participant will receive: 
  • New knowledge
  • Possibility to win a useful prize
  • Link to the workshop recording
  • Pdf of the presentation with links
  • Certificate of participation
Read the feedback from participants of previous workshops here and join us on the 20th of March!
   Online Western blot workshop with Sapphire Biosciences
Read more 2024-03-06

Agrisera at Auxentric meeting at SLU in Ume

Between the 27th and 29th of February, Agrisera attended The Auxentric Meeting, organized by Professor Stéphanie Robert, at UPSC. The meeting was held at SLU's facilities in Umeå.

Researchers from institutions around Europe were gathered at this meeting, focused on auxin transport, auxin involvement in plant development and cell shape aquisition.

Agrisera Best Talk Prize was awarded to Mark Bal from John Innes Centre, the UK, for the talk "Two auxins are better than one: Duplicated auxins in pea fruit development".

Thank you to everyone who came by Agrisera's table, for the nice chats and interesting discussions!
   Agrisera at Auxentric meeting at SLU in Umeå
Left to right: Dr. Joanna Porankiewicz-Asplund, Isabel Salén
Read more 2024-02-29

Take advantage of a new filter function to find the antibody you need

You can now filter the products in any product category, to easier find the item you are looking for.

For all Agrisera primary antibodies, you can filter by:
  • Confirmed reactivity: The antibody has been confirmed to be reactive in the species in question, experimentally
  • Predicted reactivity: The antibody has been confirmed to be reactive in the species in question, through sequence homology
  • Tested applications: The applications in which you can use the antibody in question
  • Clonality: Polyclonal/monoclonal

For all Agrisera secondary antibodies, you can filter by:
  • Host: The host species for the antibody in question
  • Conjugation: ALP, Biotin, a wide range of Fluorencent dyes, HRP and Unconjugated
  • Tested applications: The applications in which you can use the antibody in question

In case of any questions, you are always welcome to contact us!
   Agrisera filters
Read more 2024-02-28

Marchantia polymorpha - find suitable antibodies for this model species

Marchantia polymorpha is an interesting model species of large thalloid liverwort, in the class Marchantiopsida. M. polymorpha is highly variable in appearance and contains several subspecies. This species is dioicous, having separate male and female plants. It offers different advantages as a model species, including:

  • Short life cycle
  • Ease of propagation and crossing
  • High frequency of transformation
  • Haploidy
  • Small genome (approximately 280 Mb)

Agrisera offers antibodies for various proteins of this model species. Browse available antibodies here.

Can not find an antibody you are looking for? Contact us
   Marchantia polymorpha

Find out about other alternatives to Arabidopsis thaliana, in the post from Plantae.
Read more 2024-02-20

Organelle specific HSP70 antibodies

Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates, and is highly conserved throughout evolution. Hsp70 works as a molecular chaperone, and plays an important role in how cells cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70) is a highly conserved protein, and a member of the family of molecular chaperones.

Below you can fin Agrisera antibodies to plant HPS70 target isoforms, located in different cellular compartments: 
These antibodies are developed to short synthetic peptides, unique for each isoform, and each antibody has been used in numerous publications. 

Until end of March 2024 all antibodies related to environmental stress are offered with a 20% discount, when using discount code Stress20.
  
 
Agrisera anti-HSP70 (plant) antibodies
Read more 2024-02-16

Agrisera Western blot workshop in person at Uppsala University

On February 16th, Dr. Joanna Porankiewicz-Asplund, Agrisera's Technical Support Manager, will conduct a Western blot workshop in person for researchers at Uppsala University. Joanna will share her extensive knowledge and vast experience of 20+ years of using this widely employed protein analysis technique.

The workshop is going to consist of two parts, including a 10-minute break for coffee and the Agrisera Antibody and Western blot Quizzes. 

Participants will have a chance to win an Agrisera high-titer secondary antibody and 2 ECL reagents, in quizzes with questions about antibodies and the Western blot technique.

Welcome!
  Agrisera Western Blot Workshop at Uppsala University
 

Read more 2024-02-15

10% discount on all directly conjugated primary antibodies

Using directly conjugated primary antibodies will help shorten both Western blot and immunolocalization procedures, as the secondary antibody incubation step is not necessary. Agrisera offers directly conjugated, antigen affinity purified primary antibodies to proteins involved in:

These antibodies are offered directly conjugated to: 
  • Alkaline phosphatase (ALP)
  • Biotin 
  • Fluorophores: DyLight® in the range of 350 to 850 nm
  • Horse radish peroxidase (HRP)
A primary antibody must be in antigen affinity purified, to allow the conjugation to an enzyme or a dye. 
Take a look at a complete list of available directly conjugated primary antibodies here.

The 10% discount for this product group is offered until end of December 2024. 


  Agrisera directly conjugated primary antibodies for plant and algal research
 

Read more 2024-02-08

New free-of-charge images for download added!

As a gift for the plant science community, Agrisera offers over 60 free-of-charge schemes and images for download, to use on your website and in your publications!

New images added, include common plant model species, electron transfer cofactors and AGO proteins.

Please cite SciGrafik and Agrisera as the source for these images.

Browse all the images here
 Free schemes for plant science to download
Read more 2024-02-06

Agrisera distributor in India, Biogenuix on the Annual Meetiong of Plant Tisssue Culture Association

Between January 23rd and January 25th, one of Agrisera's distributors in India, Biogenuix, attended the 45th Annual Meeting of Plant Tissue Culture Association. 

The goal of this meeting was to provide a platform for students, researchers and scientists to present their work, and exchange the most recent developments in plant science. 

Agrisera's product catalog and flyers were available for participants. 





 
Biogenuix representing Agrisera at Annual Meeting of Plant Tissue Culture Association
Image courtesy of Biogenuix. 
Read more 2024-02-01

Agrisera Western blot workshop with BiotechnoLabs, Agrisera distributor in India

On January 30th, Agrisera conducted a free online Western blot workshop together with our distributor in India, BiotechnoLabs. The workshop was also opened for worldwide participation. We had the pleasure to connect with researchers from India, Czech Republic, Poland, Spain, Portugal, France, Germany and the UK.

The workshop was presented by Dr. Joanna Porankiewicz-Asplund, Agrisera's Technical Support Manager. Joanna shared her extensive knowledge and vast experience of 20+ years of using this widely employed protein analysis technique.

The workshop consisted of two parts, including a 10 minute break for coffee and the Agrisera Antibody and Western blot Quizzes, which were very well attended. Many interesting questions were asked. 

Participants also had a chance to win an Agrisera high-titer secondary antibody and 2 ECL reagents, in quizzes with questions about antibodies and the Western blot technique.

Each participant of the workshop received a pdf of the presentation, with links to additional information, as well as the recording of the workshop for the future use.  

Agrisera online Western blot workshop

If you are interested in this type of online workshop to be held at your institution, please contact us!
 Some feedback we received after the workshop: 

"Many thanks for the wonderful presentation. We really enjoyed the informative presentation."

"Thank you again for the very Interesting and Informative Workshop. I would like to ask if It would be possible for me to receive the PowerPoint, since it was very well-made, and I'd like to share it with some coworkers."

"Thank you for such an informative and helpful presentation! You've answered many questions I had (and even some I didn't know I had!) about Western Blots."
 

"I participated in your amazing presentation today and I would like to receive the recording, presentation and certificate."

Agrisera online Western blot workshop

Images of workshop participants, courtesy of Brajendra Tripathi.
Read more 2024-01-31

Planning your conference attendance in 2024? Check the Global Plant Events Calendar, for conferences, workshops and online events

The Global Plant Events Calendar, a joint initiative of American Society of Plant Biology (ASPB/Plantae) and Agriera, is providing a quick overview of conferences, congresses, smaller meetings, and online workshops and webinars, with the focus on plant science. 

Each year, over 200 events are listed, and the number is continuously growing. The listed events are curated to provide you high-quality events, and protect from irrelevant and so called "predatory" meetings. If you are organizing a meeting, you are welcome to submit it to the calendar. If you are planning your conference attendance, or search for possibilities to increase your knowledge, check the calendar!

For information on how the calendar was created, read an interview conducted by ASPB, with Agrisera's Technical Support Manager, Dr. Joanna Porankiewicz-Asplund,  here.
 Global Plant Events Calendar, supported by Agrisera

 
 

Read more 2024-01-26

Antibodies to Heat Shock Proteins - useful tools in investigastion of plant responses to environmental stress

Heat Shock Proteins (HSP), are a family of proteins that play essential roles in protecting cells from stressors, such as high temperatures, and are involved in various cellular processes. Agrisera offers specific antibodies to HSP proteins, which can aid research in different areas, such as:

  • Monitoring Stress Conditions: Antibodies to plant HSPs can be used to monitor the expression levels of these proteins under different stress conditions, such as heat stress, and lead to understanding of  the plant's response to environmental challenges.
  • Understanding Cellular Responses: Antibodies can be employed to determine the cellular localization of specific HSPs. This information is valuable in understanding how these proteins function within different cellular compartments.
  • Molecular Biology and Biochemistry: Researchers can use antibodies to detect and quantify specific plant HSPs, providing insights into their expression patterns and regulation.
  • Immunoprecipitation: Antibodies can be used for immunoprecipitation studies to isolate and analyze specific HSP-protein interactions. This helps in understanding the molecular mechanisms underlying stress responses.
  • Biotechnological applications: Understanding the role of HSPs and their regulation can aid in the development of genetically engineered plants with improved stress tolerance. Antibodies can assist in validating the expression of these proteins in genetically modified plants.
  • Biomarker development: HSPs may serve as biomarkers for stress in plants. Antibodies can be used to develop diagnostic tools for assessing the stress levels in plants, which is essential for crop management.
  • Environmental monitoring: Antibodies can be applied to monitor the levels of plant HSPs in natural environments. This can be useful for assessing the impact of environmental stress on plant populations.
The antibodies to plant HSPs are versatile tools that aid researchers in exploring the intricate details of plant stress responses, molecular interactions, and potential applications in agriculture and biotechnology. These antibodies enable scientists to investigate the roles of specific HSPs, assess stress conditions, and develop strategies for improving plant resilience.

Browse the Agrisera HSP antibody collection, offered 20% off until end of March, here

 

 Agrisera antibodies to HSP proteins, 20 % off until end of March
 

Read more 2024-01-26
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