Agrisera supported the 5th International Plant Proteomics Organization (INPPO) conference, held between the 22nd and 25th of May 2023, in Thessaloniki, Greece. The conference focused on basic and applied plant proteomics research.
|The conference Straub Days 2023, took place between 25th and 26th of May at the Biological Research Centre, Szeged, Hungary. During the conference, the poster "Plant cell immunolocalization and the use of advanced imaging techniques at HCEMM Advanced Core Facility" was presented by Dr. Ferhan Ayaydin and Dr. Divya Teja Dondapati from the Functional Cell Biology and Immunology-Advanced Core Facility, HCEMM, Szeged; and Institute of Plant Biology, BRC, Szeged.|
The poster showed various methods of material preparaton, and included immunolocalization results obtained with Agrisera antibodies to:
These were combined with matching Agrisera secondary antibodies:
The plant material used were: Oryza sativa L. ssp. japonica cv. 'Unggi 9', Zea mays hybrid variety, Arabidopsis thaliana expressing GFP, and Oryza sativa bpt-jeelakarra variety.
Sample preparation methods followed for the immunolocalization studies on dicot and monocots were: suspension culture, root squash, whole root mount and cryosectioning.
Seen in the image, left to right: Dr. Ferhan Ayaydin and Dr. Divya Teja Dondapati.
|Protein Extraction Buffer (PEB) is an extraction buffer for the disruption and solubilization of total protein from plant tissues and algal cells. The buffer contains the anionic detergent LDS, which does not form crystals while stored at 4-10°C, and is quickly ready to use. |
Expected yields will be 1.5-6 µg/µl total protein (recovered from standard procedure), depending on the starting material, e.g. its biological stage and homogenization method used (bead beater vs. sonication). For processing of multiple samples, simultaneous extraction using for example a bead beater is recommended, for consistency of extraction.
Provided as 5 x 2 ml (4x stock), Agrisera's PEB buffer allows up to 75 isolations of plant material (using 500 μl 1x PEB for 100 mg fresh weight), or 190 isolations of algal material (using 200 μl 1x PEB for cell amounts corresponding to 4-10 μg total chlorophyll).
To product page:
PEB (4x) | protein extraction buffer
| Chosen references: |
• Altuntas et al. (2020). Proline-stimulated signaling primarily targets the chlorophyll degradation pathway and photosynthesis associated processes to cope with short-term water deficit in maize. Photosynth Res. 2020 Apr;144(1):35-48. doi: 10.1007/s11120-020-00727-w.
• Pérez-López et al. (2020). Transcriptome Analysis Identifies Plasmodiophora brassicae Secondary Infection Effector Candidates. J Eukaryot Microbiol. 2020 Jan 11. doi: 10.1111/jeu.12784.
• Morin et al. (2019). Morin et al. (2019). Response of the sea-ice diatom Fragilariopsis cylindrus to simulated polar night darkness and return to light. Limnology and Oceanography. 9999, 2019, 1Ã¢??20. (sea-ice diatom)
• Bausch, A.R., Juhl, A.R., Donaher, N.A. et al. Mar Biol (2019) 166: 80.
On the 26th of May, Dr. Joanna Porankiewicz Asplund, Technical Support Manager at Agrisera, conducted a Western blot training for the sales force of Agrisera's distributor in South Africa, Bicom Biotech.
The aim was to show which are the most crucial steps of Western blot, and how to help customers using this widely employed protein analysis technique.
Good luck with helping your customers succeed with their Western blots!
As climate change becomes more noticeable, and affect all life on Earth, research on how plants respond to environmental stress intensifies in many laboratories worldwide. Agrisera antibodies, to key proteins involved in plant stress response, help solve many pressing questions of how plants tackle changes to, and threats in, their environment. New antibodies are added continuously to our collection, which includes antibodies to proteins involved in:
|On the 15th of May, Dr. Joanna Porankiewicz-Asplund, Agrisera's Technical Support Manager, conducted an online workshop for researchers from the Wageningen University & Research Laboratory of Biochemistry, The Netherlands. The title of the workshop was "Western blot - a technique possible to optimize".|
The workshop was almost two hours long, including a 10 minutes break for the Agrisera Antibody and Western blot Quizzes and coffee. Questions submitted by attendees were answered, and many pitfalls of the Western blot technique were discussed, with specific experimental examples.
Participants also had a chance to win Agrisera high titer secondary antibodies and 2 ECL reagents, in two quizzes, consisting of questions about antibodies and the Western blot technique.
Each participant of the workshop received a pdf of the presentation with links to additional information, as well as a pdf of Agrisera's Western blot guide. The recording of the workshop is available on demand.
If you are interested in this type of online workshop to be held at your institution, please contact us!
Feedback from one of the participants: "Thanks! It was a very nice and informative workshop :)"
Beside 50 µg of antigen-purified antibodies, we will also provide you with:
- High-titer, matching secondary antibodies, to be used at 1: 25 000, 1h/RT incubation
- Agrisera ECL reagents of mid-picogram or extreme low femtogram detection range
- Suggested protocol
|Product number||Product name||Uniprot ID|
|AS21 4612||BAR | Phosphinothricin N-acetyltransferase (170-183)||P16426||Epitope tags|
|AS21 4613||BAR | Phosphinothricin N-acetyltransferase (36-50)||P16426||Epitope tags|
|AS22 4832||Ble tag | Bleomycin resistance protein||P17493||Epitope tags|
|AS16 3690||Cas9 | CRISPR-associated endonuclease 9 (polyclonal)||Q03JI6||Epitope tags|
|AS22 4855||CBD-Tag | Chitin Binding Domain Epitope Tag||Epitope tags|
|AS21 4686||CBP epitope tag||Epitope tags|
|AS22 4854||CBP-tag | Calmodulin Binding Protein Eptiope Tag||Epitope tags|
|AS21 4604||E Tag||Epitope tags|
|AS22 4800||GFP11 | C-terminal of GFP||P42212||Epitope tags|
|AS21 4607||Glu-Glu Tag||Epitope tags|
|AS21 4605||Halo Tag||Epitope tags|
|AS21 4603||HSV epitope tag||Epitope tags|
|AS21 4681||HSV epitope tag||Epitope tags|
|AS21 4680||KT3 epitope tag||Epitope tags|
|AS21 4675||mStrawberry||Epitope tags|
|AS21 4602||Rho1D4/1D4 Tag||Epitope tag||Epitope tags|
|AS21 4684||S epitope tag||does not apply||Epitope tags|
|AS21 4606||SNAP/Clip Tag||Epitope tags|
|AS21 4508||Trx Tag | Thioredoxin 1 Fusion protein||Epitope tags|
Contact us with your antibody choice!
Agrisera supported the 32nd Western Photosynthesis Conference, held between the 28th and 30th of April 2023, in Bodega Biological Marine Laboratory, California, USA.
Dr. Asmit Bhowmick from the Lawrence Berkeley National Laboratory, and Grad student Albert Liu from University of California Davis.
Awaiting your free antibody choices!
For your convenience, chosen Agrisera antibodies can be purchased as sets at very favorabe pricing. The sets also include high-titer matching secondary antibodies, which can be used at 1: 25 000 1h/RT incubation, and Agrisera's ECL reagents, which offer versatile detection.
Educational antibody set for photosynthesis | Photosynthesis Tool kit – quantitation (includes standards for quantification of PSI and PSII) | Oxidative stress antibody set | Argonaute antibody set | Rubisco quantitation set (for Western blot or ELISA) | Plant Cell Compartment Antibody Marker Set for 3, 5 or 10 marker antibodies of your choice.
You are always welcome to suggest a new antibody set to us!
An example of Agrisera Antibody Set, including high-titer matching secondary antibodies and ECL reagent.
|Agrisera supported the ASPB Northeastern Section 2023 Meeting, held between the 22nd and 23rd of April, 2023, in SUNY Old Westbury, USA. The meeting was organized ny Prof. Christos Noutsos.|
The winners of Agrisera Best Poster Prizes, and a free antibody each from the Agrisera catalog, were:
Cassandra Probert from Samuel Hazen’s Lab at the University of Massachusetts, with the poster "BdSND2R is a transcriptional repressor that forms a negative feedback loop with SWAM1 to control secondary wall thickening in Brachypodium distachyon"
Alaa Mohamed Elsaeed Hassan Elminisy from Om Prakash Dhankher lab at the University Massachusetts Amherst, with the poster "Molecular and Biochemical studies on the interaction between cucumber and Plant Growth Promoting Rhizobacteria (PGPR) under Cadmium (Cd) Stress".
We are awaiting your free antibody choice!
Left to right: Cassandra Probert, Professor Christos Noutsos and Alaa M. El-Minis.
Antibodies to Arabidopsis thaliana proteins involved in photosynthesis are a core collection of the Agrisera catalog. This group of products has been developed in collaboration with research laboratories worldwide, for the last 20 years. Agrisera photosynthetic antibodies have the following features:
Beside antibodies, Agrisera has also developed Educational Posters on Photosynthesis, that are engaging and packed with up-to-date information. These posters are available for download, and as hard copies that can be requested here. Each poster was created in consultation with leading researchers from the scientific community, and the information in the posters is updated regularly. Specific figures and schemes from the posters are available free of charge for download, to be used in publications and presentations.
|Agrisera supported the 24th conference of the Austrian Society of Plant Biology (ATSPB), held between the 13th and 15th of April, 2023, in Hall in Tirol, Austria. The event was organized by the Department of Botany of the University of Innsbruck.|
The 24th conference of the ATSPB was dedicated to the importance of plants in the context of food security, biodiversity and climate change. The Agrisera Best Poster Prize was awarded to Paraskevi Charalambous, from the University of Natural Resources and Life Sciences, Vienna (BOKU), Austria, for the poster "Dissolving and Reshaping Walnut Shells: A Sustainable Material Solution?".
Awaiting your free antibody choice!
In the picture: Paraskevi Charalambous (left) and Prof Ilse Kranner, Conference President (right).
|Anti-PsbO antibodies, product number: AS06 142-33, detect a PsbO protein in a wide range of different species.|
Recently, reactivity on a new species, Eucalyptus grandis, has been experimentally confirmed using the following protocol:
13.5 µg/well of total protein extracted freshly from Eucalyptus sp. leaves. Exact buffer components were: 50 mM HEPES NaOH pH 8.0, 5mM DTT, 15 mM NaHCO3, 20 mM MgCl2, 2 mM EDTA, 4% (v/v) EDTA- free protease inhibitor cocktail (Roche Diagnostics), 4% PVPP (w/v), 20% glycerol (v/v), 1:1 Tris-buffered phenol (v/v) and 100 mM CH3COONa in methanol. Extracts were denatured with SDS beta-Mercaptoethanol sample buffer for 10 min at 90°C. Samples were separated at RT on 12% SDS-PAGE and blotted for 1 h to PVDF, using wet transfer at room temperature (RT). Blot was blocked with 5% non-fat milk for 1 h at RT with agitation. Blot was incubated in the primary antibody at a dilution of 1:5000 for 1 h at RT with agitation in TBS-T. The antibody solution was decanted, and the blot was rinsed briefly twice, then washed three times for 10 min with TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG HRP, Agrisera AS09 602) diluted to 1: 10000 in TBS-T for 1 h at RT with agitation. The blot was washed as above and developed with a following chemiluminescent detection reagent. Exposure time was 12 min.
1: 13.5 µg of Eucalyptus grandis whole leaf extract clone one (g1)
With millions of antibodies offered worldwide, by hundreds of suppliers, it may be a bit overwhelming, especially for beginners, to choose the correct antibody to answer a specific questions in a research project. Here is a short checklist with what to pay attention to:
Species reactivity – Has the antibody been used to detect the protein from the species we are working with? If not, a request should be sent to the supplier to confirm if the antibody may have so-called predicted reactivity to the target protein. If not, the antibody is not suitable, and false positive results can be produced.
Validation data – Is validation data available on a product information sheet? For non-essential proteins, validation data should include results obtained with knockdown or knockout mutants. Western blot results showing that an antibody binds to a recombinant protein, are not relevant to the binding in a specific sample, extracted from a specific tissue or organism. Here we explain why.
Recommended antibody dilution – The lower the dilution, the fewer tests can be conducted using an antibody. Here we explain why.
Publication record – Being citet in scientific articles definitely confirms the performance of a given antibody. However, as there is no worldwide standard, product numbers are oftentimes not included in the "Material and Method" section. This makes it difficult to identify what antibody has been used to obtain the presented results. How to cite antibodies in scientific publication, is explained in this short video.
You are always welcome to contact us with any questions, and we will promplty help you to find the best antibody for your specific species and application.
|The 19th International Workshop on Plant Membrane Biology (IWPMB) took place Taipei, Taiwan, between 27th and 31st of March. This meeting has been organized since 1968 and covers topics related to membrane transport in the context of signaling, physiology, biotic and abiotic stresses and development. |
Agrisera supported the meeting with two Best Poster Prizes.
The Best Poster Prizes were awarded for the scientific poster contribution of Huimin Feng from Nanjing Agricultural University, the poster title was "P02-11 Strigolactone and gibberellin signalling coordinately regulates metabolic adaptations to changes in nitrogen availability",
and Dr. Swee-Suak Ko from Academia Sinica, Biotechnology Center in Southern Taiwan with the poster "P11- 11 Redefine the Role of OsPHO1;2 Phosphate Transporter in Rice Grain Filling".
We are awaring your free antibody choices! Good luck with your research!
Well-validated antibodies to specific cellular compartments are crucial to support data regarding localization studies of novel proteins. Agrisera's Cellular compartment antibody collection contains of marker antibodies to:
Agrisera compartment marker antibodies have been used in in a wide range of publications over the last 15 years. For many compartments we offer several antibodies, to proteins of different molecular weight, to match your experimental requirements.
Not certain which compartment marker antibody to choose? Ask Agrisera's techincal staff for an advice.
|Our warmest congratulations to master student Karolina Todorska, who scored a 9/9 in the Agrisera Antibody and Western blot quiz, part 1 and part 2!|
The quiz was a part of Agrisera's lecture on Western blot, conducted by Dr. Joanna Porankiewicz Asplund on the 29th of March, as part of the course "Biochemistry: Protein Separation Methods and Analytical Techniques", held at The Department of Chemistry, Umeå University, Sweden.
As the prize, Karolina received the book "Photosynthesis – Solar Energy for Life" by Dr. Dmitry Shevela, Prof. Lars Olof Björn, Prof. Govindjee.
Good luck with your studies!
Agrisera offers an extensive and well-published collection of antibodies to support research on the biology of the model plant Arabidopsis thaliana.
Find a matching antibody to a gene ID | Complete list of antibodies with confirmed and predicted reactivity to Arabidopsis thaliana | Examples of publications with Agrisera Antibodies | Benefit from a 10% discount until end of June with the discount code Arabi10
Marker antibodies for Arabidopsis thaliana cellular compartments
Extensive collection of antibodies to Arabidopsis thaliana proteins involved in photosynthesis
Extensive collection of antibodies to Arabidopsis thaliana proteins involved in a stress response
Agrisera's Arabidopsis thaliana antibodies are suitable for a wide range of techniques, including immunofluorescence, ChIP, IP and Western Blot. Over 100 antibodies are available free of charge for testing, in return for results. Check the list here.
On the 28th of March, Agrisera participated in a Job Fair at Umeå University. The event is organized annually by Umeå Biotech Incubator, with the aim to connect students and potential new work places within the Life Science sector in Umeå.
A warm thank you to all the students who came by and asked us interesting questions. We do hope that you see Agrisera as an attractive future workplace!
Agrisera Laboratory Technician Marcus Boman and Product Developer Evelina Höök represented Agrisera at the Job Fair.
|On the 24th of March, Dr. Joanna Porankiewicz-Asplund, Agrisera's Technical Support Manager, conducted an online workshop for researchers from the Max Planck Institute of Molecular Plant Physiology in Potsdam-Golm, Germany. The title of the workshop was "Western blot - a technique possible to optimize".|
The workshop was almost 2 hours long, including a 10 minutes break for the Agrisera Antibody and Western blot Quizzes and coffee. Questions submitted by attendees were answered, and many pitfalls of the Western blot technique were discussed, with specific experimental examples.
Participants also had a chance to win Agrisera high titer secondary antibodies and 2 ECL reagents, in two quizzes, consisting of questions about antibodies and the Western blot technique.
Each participant of the workshop received a pdf of the presentation with links to additional information, as well as a pdf of Agrisera's Western blot guide.
If you are interested in this type of online workshop to be held at your institution, do not hesitate to contact us!
Feedback from one of the participants:
Thank you very much for the workshop! I myself learned quite some additional things, I didn’t know before. And I talked to some of my colleagues just now and they also really liked your workshop. Thanks also for providing the pdf, I will send them to the participants!
I really enjoyed your presentation and I can see how dedicated you are about western blots, it was really great to listen to you!
|Agrisera offers a wide range of high-titer secondary antibodies, for detection of primary antibodies from different hosts, like goat, llama, mouse, rabbit, rat and many more.|
Our secondary antibodies have the following features:
|The 20th Scientific Conference of the Phycology Section of the German Society for Plant Sciences took place in the historical Rosensäle in Jena, Germany, March 12th-15th, 2023. |
Agrisera supported the meeting with the Best Poster Prize ("1. Posterpreis der Sektion Phykologie"), the winner och which was selected by a Phycology Jury.
The Best Poster Prize was awarded to the scientific poster contribution of Trang Vuong, entitled "Life of Chlamydomonas reinhardtii in a 3-D structured environment", authored by Trang Vuong¹, Ece Kurtoglu¹, Volker Wagner¹, Somak Chowdhury², Ruchira Mukherji², Pierre Stallforth² and Maria Mittag¹.
¹Matthias Schleiden Institute, Friedrich Schiller University Jena, Am Planetarium 1, 07743 Jena, Germany
²Department of Paleobiotechnology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute (HKI), Beutenbergstraße 11a, 07745 Jena, Germany
Trang Vuong is a PhD student in the laboratory of Maria Mittag. She has a fellowship of the Carl Zeiss Foundation and is a student member of the DFG-financed Cluster of Excellence "Balance of the Microverse".
Poster price award, 1st place. From left to right: Trang Vuong, Prof. Dr. Andreas Holzinger. Photograph by: PD Dr. Volker Wagner
|Agrisera supports protein detection in your laboratory with tailored reagents with the following features: |
For chemiluminescent detection of the activity of HRP (Horseradish Peroxidase enzyme), coupled to a secondary antibody, we recommend using:
AgriseraECL Bright (AS16 ECL-N), for low pico to mid-femtogram detection (for proteins of medium and high expression), or the enhanced AgriseraECL SuperBright (AS16 ECL-S), for extreme low femtogram detection (for proteins of low expression). Both reagents are provided in one set and if one of ECL is not sensitive enough, or there is too much background, the membrane can be washed and developed again, as described here.
For laboratories without a CCD camera, visualization of the target protein can be made using so-called chromogenic detection with Agrisera BCIP/NBT ALP Substrate (AS19 BCIP-NBT) or Agrisera BCIP/NBT Plus ALP Substrate solution (AS19 BCIP-NBT-PLUS). In this case, the secondary antibody is conjugated to an enzyme called ALP (Alkaline Phosphatase). The reaction is visualized directly on the membrane through color development, and the membrane can be stored in the lab book for a future reference. This detection system is not appropriate for proteins of low expression, or to detect protein modifications, such as phosphorylation.
The 20th Scientific Conference of the Phycological Section of the German Botanical Society took place between 12th and 15th of March in Jena, Germany.
The talks covered various aspects of algal biology, including biotic interactions, algal ecology, perception of abiotic factors, applied phycology, algal physiology and algal tool box.
Agrisera supported the conference with The Best Poster Prize and free copies of Agrisera's Educational Posters, focused on photosynthesis.
|Agrisera secondary antibodies, conjugated to HRP (Horseradish peroxidase) and ALP (Alkaline phosphatase) enzymes, are high-performance antibodies that can be used in high dilution, up to 1: 100 000. |
This means that 1 mg of a given antibody will allow quite a number of experiments to be conducted.
The most common mistake is comparing secondary antibody pricing between various suppliers, when antibody performance is the actual key. Using secondary antibodies in high dilutions also minimizes potential background issues.
Agrisera secondary antibodies are high-performing and price-worthy, delivered in an environmentally-friendly way without dry ice, which is costly to produce and transport.
You are welcome to check the stock balance of any secondary antibody you need, at any time, here. For the secondary antibodies that are not in stock, the delivery time is around 5-10 business days.
During the entirety of 2023, all Agrisera secondary antibodies labelled with ALP and HRP are provided with a 10% discount. Use discount code Conj10
Agrisera antibodies perform above market average in terms of dilution.
Glutamine synthetase GlnA is the key enzyme in the incorporation of mineral nitrogen into glutamine. The activity of this enzyme is controlled by adenylarion under conditions of abundant glutamine.
Beyond this, reactivity is predicted to the following species, based on sequence homology: Alpha, beta, gamma proteobacteria, Arthropsira sp. PCC 8005, Crenarchaeotes, Enterobacteria, Escherichia coli, Euryarchaeotes, Thermotogales
Confirmed and predicted reactivity is explained further here.
3 µg of total protein from Trichodesmium IMS 101 extracted with Agrisera Protein Extration Buffer (AS08 300) (1-8) and GlnA protein standard 0.3, 0.15, 0.07 pmol (9-11) were used to visualise and quantite GlnA.
|Agrisera's product information sheets contain the most up-to-date information about:|
Just in the year 2022, we have added 305 selected citations to our product information sheets.
New information is updated on a weekly basis, to provide you with the most up-to-date product information sheets. In case of any questions, you are always welcome to contact Agrisera Support.
|Between the 21st and 23rd of February, Agrisera participated in the 3rd INUPRAG symposium, held in Umeå, Sweden. This is a meeting of researchers from three collaborating institutions, namely Umeå Plant Science Centre (Sweden), INRAE (France) and CRAG (Spain). |
150 researchers, 34 posters and wide range of research topics:
Agrisera contributed with The Best Poster Prize, awarded to Dr. Marti Quevedo, former post doc in Dr. Åsa Strand group at UPSC. The poster title was: "Chloroplast retrograde signals modulate chromatin dynamics during plant greening".
Thank you for interesting discussions, suggestions and ideas!
Nitrogenase NifH is involved in the biological fixation of atmospheric nitrogen to ammonia. Alternative protein names are Nitrogenase component II, Nitrogenase Fe protein, Nitrogenase reductase, and FeMoCo-nitrogenase.
Beyond this, reactivity is predicted to the following species, based on sequence homology: Azotobacter vinelandii (Gram-), Bradyrhizobium japonicum, Cyanobacteria, Cyanothece ATCC51142, Desulfotomaculum reducens (strain MI-1), Clostridium cellobioparum, Enterobacter, genera, euryachaeotes, Klebsiella pneumonia, Magnetococcus sp., Methanobacterium thermoautotrophicum, Methanococcus maripaludis, Methylobacterium sp., Mesoorhizobium loti, Rhodopseudomonas palustris TIE-1 strain, alpha, gamma, beta proteobacteria, enterobacteria, low GC gram+, high GC gram+, able to fix atmoshperic nitrogen, Rhizobium meliloti
Confirmed and predicted reactivity is explained further here.
Total Trichodesmium sp. protein extract (lanes 6-11, 80 pmol chlorophyll loaded) extracted with PEB (AS08 300), and NifH protein standard (lanes 1-5, 0.05, 0.1, 0.3 0.75 and 1.5 pmol standard loaded)
|The 36th conference Molecular Biology of Plants, organized by the Section Plant Physiology and Molecular Biology of the Germany Society for Plant Sciences took place the 6th-9th of February, North Rhine-Westphalia, Germany. The organizers were Prof. Dr. Ute Höcker (Köln), Prof. Dr. Andreas Meyer (Bonn), and Prof. Dr. Marcel Quint (Halle).|
Agrisera supported the meeting with The Best Poster Prize, a quiz on antibodies and Western blot, as well as hard copies of the Agrisera Educational Posters. The Best Poster Prize with the title "Shade signals as essential components of host detection in the parasitic plant Cuscuta campestris" was awarded to Dr. Beatrix Enderle, a postdoc in Prof. Hiltbrunner Lab at the University of Freiburg, Germany.
The winner of Agrisera Antibody Quiz was PhD student Srijana Raj from the Martin Luther University of Halle-Wittenberg, Germany. She will receive Agrisera high-titer secondary antibodies, goat anti-rabbit IgG, HRP conjugated, to be used at 1: 25 000 1h/RT, and a set with two ECL reagents of mid picogram and extreme low femtogram detection.
Thank you all who participated!
|Agrisera IncuBlocker is an "all in one" set for fast Western blot processing. The whole process takes place in a single step, as blocking, as well as primary and secondary antibody binding, occur simultaneously, followed by a wash step with included buffer. Depending on the titer of the primary antibody, it can be re-used up to 5 times (not recommended for quantification work). This means that the processing time can be shortened considerably, to 1.5 hour. |
Add your primary antibody and use your choice of chemiluminescence or chromogenic method to visualize the reaction.
The set is provided for primary antibodies developed in chicken, mouse and rabbit, and is recommended for antibodies to proteins of high and moderate expression.
How to know the expression levels of your target protein?
Check in the Protein Abundance Database.
The example above shows how the signal with a primary antibody can be visualized using IncuBlocker. No washes in between, as blocking and primary and secondary antibody incubationsn are done in one step.
|On the 19th of January, the 14th UCMR Day 2023 took place at Umeå Centre for Microbial Research, Umeå University. The meeting had outstanding talks and poster presentations, giving the possibility to present specific projects in the form of elevator talks. |
There were 50 poster presentations at the conference. A jury selected the top three posters, which were awarded prizes sponsored by UCMR and Agrisera.
Congratulations to these three articulate and ambitious researchers!
1. Winner of the best poster reward: PhD student Sandra Holmberg
2. Second place, the best poster: Dr. Dale Corkery
3. Third place, the best poster: Tom Guest
The winners are awarded an antibody of their choice from the Agrisera catalog.
Congratulations and awaiting your free antibody choice!
Agrisera goat anti-rabbit IgG secondary antibodies, conjugated to horseradish peroxidase (HRP), perform in dilution > 1: 25 000 in Western blot and ELISA, which is supported by a long list of publications.
To the product: 1 mg or trial size.
A given laboratory may aim to detect different proteins of varied expression levels, in the tissue being analyzed. Agrisera offers a unique pack of combined reagents, with two different sensitivities. This allows more versatile detection, of high and medium abundancy proteins (Agrisera ECLBright), as well as low abundancy proteins (AgriseraECL SuperBright). Depending on the target protein that we aim to detect, different detection reagent needs to be used, and Agrisera offers this possibility provided in the same set.
In the example above, the aim was to detect a highly abundant photosynthetic protein, PsbA. Using Agrisera anti-PsbA antibodies (AS05 084), a matching secondary antibodies of high titer, goat anti-rabbit, HRP-conjugated (AS09 602) and Agrisera ECL reagent of extreme low femtogram detection range (AgriseraECLSuperBright), resulted in prominently increased background signal. Choosing the other reagent, provided in the Agrisera ECL set, gave the possibility to optimize the result in a very swift manner. There is no need to repeat the whole procedure, as the membrane can be subjected to a wash and developed with less sensitive ECL. It is oftentimes not the primary antibody that contributes to increased background, but rather high load/well, combined with a too sensitive detection reagent.
We are always keen to discuss your results and advice promptly. Contact Agrisera support either using a chat button on the website or by email to [email protected].
|On the 23rd of January, Agrisera conducted the workshop "Western blot – a technique possible to optimize" for researchers and students at the Department of Biological Sciences, Wrocław University, Poland. The workshop was held by Dr. Joanna Porankiewicz Asplund, Agrisera's Technical Support Manager. The meeting gathered 80 participants and was 2 hours long. During the break, the attendees could participate in a quiz, and had a chance to win two ECL reagents with different sensitivities (AS18 SecondaryECL), or a book on photosynthesis. All attendees are very welcome to contact Agrisera with any Western blot and antibody-related questions you may have in the future. Agrisera's Western blot troubleshooting guide, antibody production posters, educational posters and pens were offered to all who attended. |
Thank you for your particiption and valuable insights!
Secondary antibodies conjugated to HRP (Horseradish peroxidase) and ALP (Alkaline phosphatase) enzymes are most frequently used to visualize target proteins in Western blot, ELISA and immunolocalization. When using secondary antibodies conjugated to HRP, the detection is performed using chemiluminescence (recorded by a CCD camera or another imager), which gives high sensitivity and good resolution in both Western blot and chemiluminescent ELISA. When using antibodies conjugated to ALP, visualization of the antibody binding to the target is achieved using chromogenic reagents, and no CCD camera is necessary.
Agrisera's secondary antibody collection is composed of over 220 antibodies; both highly purified and available in a basic format, suitable for Western blot, as well as adsorbed to serum from a wide range of animal species, suitable for immunolocalization and ELISA. Based on the analysis of antibody performance in available publications, conducted by CiteAB, it became very clear that Agrisera secondary antibodies are performing above market average. This means that Agrisera secondary antibodies conjugated to ALP or HRP can be used at high dilutions and are price-worthy reagents, and will provide you with a consistent performance over many experiments.
We are always eager to provide prompt advice on which Agrisera secondary antibody is best suited for the experiments you aim to perform.
Contact us at [email protected]!
Today, The Global Plant Events Calendar is the most comprehensive list of worldwide events for the plant community.
If you are not familiar with the calendar, this short video will show you how to use it. You are welcome to submit a meeting you are organizing!
Stay informed! Follow the calendar on Twitter @PlantSciEvents
|On the 18th of January, Agrisera conducted the workshop "Western blot – a technique possible to optimize" for researchers and students at the Institute of Experimental Biology, Adam Mickiewicz University, Poznań, Poland. The workshop was held by Dr. Joanna Porankiewicz Asplund, Agrisera's Technical Support Manager. The meeting, 2 hours long, was interactive, with lots of valuable questions. During the break, the attendees could participate in a quiz, and had a chance to win two ECL reagents with different sensitivities (AS18 SecondaryECL), or a book on photosynthesis. All attendees are very welcome to contact Agrisera with any Western blot and antibody-related questions you may have in the future. Agrisera's Western blot troubleshooting guide, antibody production posters, educational posters and pens were offered to all who attended. |
Thank you for your particiption and valuable insights!
Broad species reactivityAgrisera antibodies to enzymes involved in nitrogen metabolism have the following, unique features:
Nitrogen metabolism is one of the fundamental processes in plants, as nitrogen is a constituent of amino acids, proteins, enzymes, vitamines, alkaloids and even some growth hormones. Agrisera's nitrogen metabolism antibody collection targets key enzymes of nitrogen metabolism in plants.
Browse the whole colleaction here.
Whole collection is offered with a 20% discount until end of March. Discount code is Nitro20
This year, Agrisera supported 25 worldwide meetings.
10 awardees can choose any free antibody from Agrisera's Antibody Collection and three copies of the book "Photosynthesis – Solar Energy for Life" were awarded to winners of the Agrisera Quiz.
Over 2000 of Agrisera Educational Posters were shipped worldwide, or distributed on conferences.
Hundreds of participants attended Agrisera's Western blot workshop, "Western blot – a technique possible to optimize", either in person or online.
We are happy to contribute to the growth of the scientific community worldwide, and support meetings, workshops and conferences.
We hope to continue our support in 2023, and meet you at various events, either online or in person!
Coming conferences can be viewed in The Global Plant Events Calendar, supported by Agrisera and maintained by Plantae.
|Our warmest congratulations to Dr. Sara Raggi who received the Umeå Plant Science Centre (UPSC) Agrisera Prize 2022. Sara was awarded the prize to acknowledge her significant scientific contributions to plant cell and developmental research, as well as her commitment to creating a positive and collaborative work environment at UPSC. The UPSC Agrisera Prize is awarded every year to a person working at UPSC, and this year's prize was announced on the 7th of December during the virtual UPSC Christmas Celebration.|
Sara Raggi started to work as a postdoc in Prof. Stéphanie Robert’s group at UPSC in 2016 after finishing her PhD. She is involved in many scientific projects and is a co-author of several articles. One of her main projects has been to develop an automated method to measure the angle of unbending Arabidopsis seedlings after germination. This method uses machine learning as basis for the image analysis, and was created together with an engineering master student who Sara supervised.
Besides her scientific achievements, Sara Raggi has been actively engaged in improving the work environment at UPSC. She is the postdoc representative, member of the greenhouse introduction team and has helped with outreach events. She has taken over responsibilities in different groups at UPSC, e.g. the gardening group, who set up boxes to grow vegetables in front of UPSC, and the "Greener UPSC" group that aims to promote sustainability at UPSC, and helps organize social events.
Every year, anyone working at UPSC can nominate a colleague for the award, and the prize is a travel voucher, sponsored by Agrisera. Based on the written nominations, the members of the UPSC board voted to select the winner. This year, the UPSC Board received 11 nomination letters for seven candidates. Sara was chosen as the winner based nomination letters emphasizing her teamwork skills, scientific excellence and contribution to making UPSC a great place to work.
Over the holidays, the Agrisera office will be open on all weekdays except the 26th of December and 6th of January.
The last day for shipments in 2022 will be on the 22nd of December. Shipments will resume on January 2nd 2023.
Warm congratulations to PhD students Palwasha Baryalai, Department of Molecular Biology at Umeå University, and Eduardo Rodriguez Soldado, Umeå Plant Science Centre (UPSC), Department of Plant Physiology, Umeå University! They were awarded the Agrisera Best PhD Presentation Award at KBC Days 2022, for their elevator talks and visual abstracts.
Palwasha presented her work "Role of bacterial vesicle associated toxin in potential treatment of cancer", and Eduardo's talk and visual abstract title was "What makes a tree a tree?".
The elevator talks were 2 minutes long, and the visual abstracts had specific criteria to fulfill. The winners were chosen by an evaluation committee, who graded performance very carefully. As Palwasha and Eduardo both performed very well, it was decided to award two winners.
|Between the 8th and 9th of November, Agrisera attended the KBC Days, an annual interdisciplinary conference, taking place in person, after having been a digital event for the last two years.|
KBC is the Chemical Biological Center of Umeå University, and this year the center celebrated its 15-year anniversary. To commemorate this milestone, there was a photo contest with the title "KBC is...", where the participants were invited to share their vision of what KBC is for them.
The main theme of the 2022 KBC Days conference was "Big data", and the talks from different areas discussed advantages and challenges of collecting, analyzing and interpreting big data.
PhD students held 2 minute elevator picthes, presenting their work, accompanied by visual abstracts. The evaluation committee found it hard to choose only one winner of the Agrisera Best PhD Presentation Prize, and awarded two winners!
Thank you to all who came by Agrisera's table to talk antibodies, collect Agrisera Educational Posters, and discuss new antibody ideas. It was great to be able to meet in person again, and we are already looking forward to the 2023 KBC Days!
Agrisera will be holding the technical workshop "Western blot – a technique possible to optimize" for KBC, on the 22nd of November, between 13.00 and 15.00, in person as well as online.
If you are attending this workshop, you are welcome to send in your Western blot questions in advance!
|Boost your research! |
Agrisera offers over 80 free-of-charge antibodies for testing, for a wide range of different plant and algal proteins.
Beside 50 µg of antigen purified antibodies, we will also provide you with:
|Our warmest congratulations to young researcher Katarina Šoln from the Biotechnical Faculty, at the University of Ljubljana, Slovenia. She was awarded the Agrisera Best Poster Prize, during the 5th Conference on Plant Proteases, held between the 5th and 7th of September in Ljubljana, Slovenia.|
Good luck with your research!
We are awaiting your free antibody choice!
|We are happy to announce that the Agrisera Educational Poster 6 "Plant small RNAs: Biogenesis and Function" is available and can be either requested as a free hard copy and delivered to your mailbox, or downloaded as a pdf from the Agrisera website. |
Agrisera's Educational Poster Series came to life in 2016, as a close collaboration project between Agrisera, researchers in the field and Dr. Dmitry Shevela (SciGrafik). Posters 1-5 are focused on photosynthesis, while we are moving into another research area with poster 6, namely: plant small RNAs including microRNAs. Poster 6 has been prepared in collaboration with Dr. Blake Meyers, Dr. Junpeng Zhang and Dr. Keith Slotkin from the Donald Danforth Plant Science Center. Separate figures from poster 6 will be available for download in October.
The poster 6 launch is accompanied by 15 % off all small RNA and microRNA related antibodies until the end of 2022.
Use discount code: Micro15