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Between 19th and 22nd of September Agrisera participated in 11th confrence of Polish Society of Experimental Plant Biology held in Poznań, Poland.
Out of 165 posters, the Best Poster Prize was given to Joanna Wójtowicz, for the poster with the title> “Unraveling the Role of Proteins, Lipids, and Pigments in Etioplast Prolamellar Body (PLB) Formation” The awardee of the 3rd place, Zofia Szlachtowska, received from Agrisera the book on photosynthesis. | ![]() From the left: Joanna Wójtowicz (awardee), Joanna Porankiewicz Asplund (Agrisera), Michał Rurek (Organizing cometee) |
The success of your research has been our focus for over 20 years. If you require antibodies to support your project, that are not available anywhere, you are always welcome to contact us with your antibody suggestion. We will promptly evaluate if it is feasible to produce an antibody to the protein you aim to detect. If it is, we will provide you with 50 µg of this antibody, antigen affinity purified and completely free of charge, within a couple of months. Please send us the gene ID or protein sequence in FASTA format for your suggested target, and we will promptly let you know if an antibody to this protein target can be included in the Agrisera catalog. Antibodies that are currently under development and available for testing are collected here. | ![]() |
CCD cameras are often used for visualizing the binding of an antibody to a target protein, through chemiluminescent or fluorescent detection. In cases where a CCD camera is not available in the laboratory, secondary antibodies conjugated to alkaline phosphatase (ALP) can be used, and the reaction can instead be visualized using Agrisera TMB reagents. This way, medium and high abundancy proteins can be visualized easily, and the obtained results can be saved in the lab book (although please remember that there is a fading effect). If you are not certain which secondary antibody to choose, our technical team will promptly answer all your questions. | ![]() |
TurboID is a proximity labeling technique used in cell biology and molecular biology research to study protein-protein interactions and subcellular protein localization. This approach has many great benefits, such as:
| ![]() Agrisera offers an excellent polyclonal antibody to TurboID. Check product information sheet here. |
Agrisera supported the 6th Plants and People (P&P) Conferences, organized by PhD students at the Max Planck Institute of Molecular Plant Physiology, in Potsdam-Golm, Germany, between the 6th and 7th of September. | ![]() |
Since Agrisera's start in 1985, we have always delivered on our promises, and if there are ever any delays in shipments, we inform you very promptly. | ![]() |
Agrisera Educational Poster 7 is the second poster in the series on epigenetics. The content was developed by researchers in the field, namlely Dr. Blake Meyers and Dr. Junpeng Zhan from the Donald Danforth Plant Science Centre (USA), Dr. Nicolas Bologna from the Centre for Research in Agricultural Genomics (Spain), and Dr. Rebecca Mosher from the University of Arizona (USA). The contents were transferred into the graphical representation by Dr. Dmitry Shevela (SciGrafik), (Sweden).
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Agrisera supported the 4th Brassinosteroid meeting, which took place between the 16th and 18th of August in Ghent, Belgium. | ![]() |
Agrisera antibodies can be found in thousands of scientific publications, with a publication record dating back to the year 1999. Agrisera was awarded "Plant Science Antibody Supplier of the Year" by CiteAb. This is an award that gives light to the supplier with the most antibody citations related to plant science. We are glad that Agrisera antibodies are supporting research in plant science, and promise to work hard to provide the community with an even wider range of quality products. Below is a list of some recent scientific articles, citing Agrisera antibodies:
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Plant science has a plethora of species that are used as model organism. Arabidopsis thaliana: Perhaps the most widely used model species in plant science. It has a small genome, a short life cycle, and is easy to grow in a laboratory setting. A. thaliana has provided invaluable insights into plant genetics, development, and responses to various environmental factors. Oryza sativa (rice): A staple food for a large portion of the world's population. Its relatively small genome, and genetic similarities to other grass species, make it an important model species for studying crop genetics, as well as aspects of plant development and responses to stress. Zea mays (maize): A major cereal crop. Its large size and genetic diversity have made it an important model species for studying genetics, development, and traits related to agriculture and bioenergy production. Medicago truncatula: A model species for studying legumes. Legumes are important for their ability to fix nitrogen, as well as their nutritional value. Medicago is used to understand symbiotic relationships between plants and nitrogen-fixing bacteria, called rhizobia. Physcomitrium patens: A model species for studying plant development and evolution. Mosses are simple plants that offer insights into early land plant evolution, and the transition from aquatic to terrestrial environments. Brachypodium distachyon: A model grass species that is closely related to important cereal crops, like wheat, barley, and oats. It is used to study traits related to grain crops, including growth, development, and stress responses. Nicotiana benthamiana: Often used for research in plant molecular biology, due to its relatively large leaves and the ease with which it can be transformed for genetic studies. Chlamydomonas reinhardtii: A single-celled green algae, widely used as a model organism for studying photosynthesis, cell biology, and genetics. It has contributed significantly to our understanding of chloroplast function, and has been explored for biofuel production. Diatoms: A major group of algae known for their intricate silica cell walls. They are important primary producers in aquatic ecosystems and play a vital role in the global carbon cycle. Cyanobacteria (Blue-Green Algae): Often studied alongside algae, due to their similar ecological roles and photosynthetic abilities. They have been researched for their role in nitrogen fixation, harmful algal blooms, and potential use in biofertilizers and biofuels. ... and many more species! With this in mind, Agrisera continuously develops new antibodies with broad species reactivity. Get an overview of antibodies for over 30 different species here. | ![]() Agrisera Search by Species |
Agrisera attended the Plant Biology 2023 conference (PB23), in Savannah, GA, USA, held between the 5th and 9th of August. Agrisera was represented by Edith Kalén and Chris Brown. |
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Benefit from Agrisera's high-titer goat anti-rabbit, HRP-conjugated secondary antibodies, which offer the following advantages:
Use discount code Conj10 and benefit from a 10% discount until end of 2023! | ![]() Comparison of Agrisera's high-titer goat anti-rabbit HRP-conjugated secondary antibodies with a secondary antibody from another supplier. More info: Do secondary antibodies differ only in pricing? |
The flowering process in plants is a crucial stage in their life cycle, where the reproductive structures called flowers are produced and regulated, both by internal genetic factors and external environmental cues. Agrisera's antibody collection for plant developmental biology contains antibodies for proteins involved in plant flowering, such as: Let us know and be the first to use it, free of charge! | ![]() |
Does your lab use the Western blot technique as a standard protein analysis method? If so, take advantage of the fact that Agrisera has over 20 years of knowledge regarding this complex and most frequently used technique. How to do it? Contact [email protected] to book a webinar with Agrisera's Techncal Support Manager. The webinar date and time will be chosen fitting to your and your colleagues schedule. Duration: 2 hours with a break Included: Agrisera Quiz with useful prizes Every attendee will receive:
Book your webinar today! | ![]() |
Loading control antibodies are used to assess if the protein samples have been loaded equally across the gel, as well as transferred to the membrane. Such controls need to be ubiquitously and constitutively expressed proteins. Commonly used loading controls are the following proteins: actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and tubulin. Actin and tubulin are highly conserved proteins, which play essential roles in the plant cytoskeleton. Actin is involved in cellular processes, such as cell division, cell share maintenance and intracellular transport. | Agrisera loading control antibody collection contains:
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Together with Agrisera's exclusive distributor in the UK, Newmarket Scientific, Agrisera organized an online Western blot workshop on the 19th of July. Dr. Joanna Porankiewicz-Asplund, Agrisera's Technical Support Manager, shared her extensive knowledge and vast exprience of 20+ years of using this widely employed protein analysis technique. The participants in the workshop were from the University of Cambridge and the University of Glasgow and Nottingham. The workshop consisted of two parts, including a 10 minute break for the Agrisera Antibody and Western blot Quizzes and coffee. Questions submitted by attendees were answered, and many pitfalls of the Western blot technique were discussed, based on specific experimental examples. Participants also had a chance to win Agrisera high titer secondary antibody and 2 ECL reagents, in quizzes with questions about antibodies and the Western blot technique. Each participant of the workshop received a pdf of the presentation with links to additional information. The recording of the workshop is available on demand. | ![]() Feedback from the participants: "Thank you so much for this great workshop." If you are interested in this type of online workshop to be held at your institution, please contact us! |
Agrisera supported the Portuguese Spanish Congress of Plant Biology 2023, which took place between the 9th and 12th of July in Braga, Portugal. Congress topics covered diverse fields, like the adaptation of plants, biotic and abiotic stresses, molecular mechanisms that control plant development and function, plant biotechnology and sustainable crop production.
The winners of a free book about photosynthesis in Agrisera's online Quiz about antibodies and Western blot were:
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Green Fluorescent Protein (GFP) of the jellyfish Aequorea victoria, that lived on Earth for millions of years before being discovered, was first purified and characterized in 1962, by Osamu Shimomura, a Japanese organic chemist and marine biologist. GFP is excited by light in the blue/violet/ultraviolet portion of the spectrum, and emits light in the green portion (hence the name). The protein emits light by undergoing a process called photobleaching, where it absorbs light energy and then re-emits it as fluorescence. Tagging a target protein with GFP allows its visualization, and the detection of its transgenic expression in vivo, as well as its subcellular localization, cell visualization and investigation of protein-protein interactions. It can be used for cell tracing and tracking. This easily detectable fluorescence has made GFP an invaluable tool in various biological studies, enabling researchers to visualize and understand complex biological processes in real-time. ![]() Image: Rabbit polyclonal antibody to GFP, AS20 4443 used on roots of Arabidopsis thaliana. Agrisera's antibody collection to Green Fluorescent Protein (GFP) and its variants (EGFP, S65T-GFP, RFP like mCherry, RS-GFP, YFP and Venus) includes both, polyclonal and monoclonal antibodies with different labels, enzymatic and fluorescent, which detect both native and denatured GFP. | How is GFP used in plant science?
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Agrisera supported the 6th PostDoc Day organized by postdocs from the Faculty of Sciences at the University of Geneva, Switzerland. The goals of the event were to strengthen social and scientific ties between the postdocs, and to promote career development. Agrisera Best Talk Prize was awarded to Dr. Hepeng Yao. | ![]() |
Together with Agrisera's exclusive distributor in the UK, Newmarket Scientific, Agrisera organized an online Western blot workshop on the 12th of July. Dr. Joanna Porankiewicz-Asplund, Agrisera's Technical Support Manager, shared her extensive knowledge and vast exprience of 20+ years of using this widely employed protein analysis technique. The participants in the workshop were from the University of Glasgow (Scotland), Imperial College of London (England) and University College Cork (Ireland). The workshop consisted of two parts, including a 10 minute break for the Agrisera Antibody and Western blot Quizzes and coffee. Questions submitted by attendees were answered, and many pitfalls of the Western blot technique were discussed, based on specific experimental examples. Participants had a chance to win Agrisera high titer secondary antibody and 2 ECL reagents, in quizzes with questions about antibodies and the Western blot technique. Each participant of the workshop received a pdf of the presentation with links to additional information. The recording of the workshop is available on demand. If you are interested in this type of online workshop to be held at your institution, please contact us! | ![]() Feedback from the participants: "The feedback from your workshop was quite good our students really enjoyed it and said it was very practical and addressed real questions." "Thank you very much for the prizes and the information of the workshop. As a starter to the western blot world, the knowledge that has been delivered from the workshop really helped me build up a general understanding of western blot as well as trouble shooting the potential problems. I love the FAQ part of each section as I hold the same questions many times." |
Recently we received the following question: "I would like to know whether you have a GFP antibody which works well to detect fusion proteins generated in plants? The GFP we used is eGFP from Aequorea victoria. We tried anti-GFP antibodies from other suppliers, but neither works well for our protein driven by native promoter." Here is our answer: Yes, Agrisera offers both polyclonal and monoclonal antibodies to GFP (Green Fluorescent Protein), which detect fusions with plant proteins. Below are links to some of these products. Anti-Venus polyclonal antibodies Anti-GFP, polyclonal antibodies | | ![]() |
Agrisera participated in the Plant Biology Europe conference, between the 3rd and 6th of July, in Marseille, France. The event was attended by 400 participants from different countries in Europe, as well as from South Korea. Particular focus of the conference was given to plants and climate change, algal biology and bioenergy. We hope that you have enjoyed Agrisera's Educational Posters, as well as the Agrisera Quiz, which consisted of six questions, with a free Agrisera antibody as the first prize. The lucky winner of the quiz was Dr. Haemyeong Jung from Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB).We are looking forward to the next Plant Biology Europe meeting in 2025! | | ![]() |
Agrisera's developmental biology antibodies include hundreds of antibodies to investigate various aspects of plants, including: Circadian clock | Cytiskeleton | Flowering | Ion metabolism | Lipid metabolism | Photomorphogenesis | Senescence/cell death | Plant Signal Transduction This unique and comprehensive collection, validated on specific material, is available with a 20% discount until end of September 2023. Agrisera antibodies are validated using:
The Agrisera catalog of antibodies is constantly expanding. Suggest a new antibody and be the first to use it! | ![]() |
Agrisera participated in the Nordic Photosynthesis Congress and Nordic Algae Symposium, between the 19th and 21st of June, at Umeå University, Sweden. At the event there were 119 researchers present, and 32 posters covering this year's theme "From Photosynthesis to Industrial Applications". A group of conference participants from Germany and Finland visited Agrisera's facility in Umeå during the evening of the second day of the event. Two Agrisera Best Poster Prizes, and a free antibody each from the Agrisera catalog, were awarded to:
The book "Photosynthesis, solar energy for life" was awarded to Shaikh Mohd Kashif from Univeirsity of Gothenburg as the first prize in a Quiz about proteins, plant science and antibodies, conducted by Agrisera. Thank you to all the conference participants for interesting discussions, suggestions and ideas! | ![]() 1. Agrisera's table at the conference. 2. Researchers from Finland and Germany outside of Agrisera's facilities during a visit. 3. Left to right: Malene Olsen (winner Best Poster Prize), Lenny Maliham-Yap (winner Best Poster Prize), Shaikh Mohd Kashif (winner Agrisera quiz), Joanna Porankiewicz-Asplund (Agrisera's conference representative and Technical Support Manager). |
Material: Zea mays hybrid variety Preparation 5 days-old germinating maize root tips were cut and fixed with 4% formaldehyde, 60 min, RT. Cell walls were digested with enzymes cellulase and pectinase in MES buffer for 90 min, RT. After washing with PBS, roots were squashed gently using a flat tip forceps to release the cells into PBS buffer. Cells were allowed to settle O/N at 4°C, followed by immunolocalization. Immunocytochemistry (ICC) Cells were permeabilized with 0.5% Triton X-100, 10 min, RT, followed by washing with PBS buffer before blocking with 5% fish gelatin-PBS, 30 min, RT. Detection antibody: Cells were incubated with rabbit anti-clathrin 1,2 primary antibodies conjugated with DyLight®594 (AS10 690-DL594, Agrisera) for 3h/RT. Nuclei were stained with DAPI followed Fluoromount-G mounting (Southern Biotech). Courtesy of Dr. Ferhan Ayaydin and Dr. Divya Teja Dondapati, Hungarian Centre of Excellence for Molecular Medicine, (HCEMM), Szeged, Hungary. | ![]() Upper panels show the Differential Interference Contrast (DIC) transmitted light image (gray) and Clathrin immunodetection (red). Lower panels represent DAPI labelled nuclei (cyan) and the merged image of Clathrin and DAPI. |
Agrisera attended the 33rd International Conference on Arabidopsis Research (ICAR2023) in Chiba, Japan between the 5th and 8th of June, 2023. A big thank you to everyone who came by Agrisera's booth to talk antibodies and plant science! We would also like to give a big thank you the organizers and to our Japanese distributor Funakoshi, who accompanied us in the booth and invited us for a lovely meeting and dinner in Tokyo! Thank you to Ms. Ayuna Kisanuki, Mr. Akihiro Yoshida, Mr. Koichi Tanaka, Mrs. Sachie Kai and Ms. Aoi Sawada! Agrisera's mascot and antibody expert Buddy was also present at the conference. ![]() | ![]() Agrisera's conference booth at ICAR2023. ![]() Left to right: Akihiro (Funakoshi), Edith (Agrisera), Ayuna (Funakoshi), Koichi (Funakoshi). |
Various antibodies to epitope tags have been used for immunoifluorescent localization of target proteins in transfected HEK293 cells, including anti-HA (AS12 2220), anti-3xHis (AS20 4441) and anti-Myc (AS21 4529). Details of employed protocols are provided on the specific antibody product pages.
Images courtesy of Courtesy of Dr. Anna K Överby, Molecular Infection Medicine Sweden (MIMS), Section of Virology. Department of Clinical Microbiology Umeå University, Sweden.
On the 12th of June Dr. Joanna Porankiewicz-Asplund, Agrisera's Technical Support Manager, conducted an online workshop for researchers from the Centre for Research in Agricultural Genomics (CRAG), Spain. The title of the workshop was "Western blot – a technique possible to optimize". The workshop was almost two hours long, including a 10 minutes break for the Agrisera Antibody and Western blot Quizzes and coffee. Questions submitted by attendees were answered, and many pitfalls of the Western blot technique were discussed, with specific experimental examples. Participants also had a chance to win Agrisera high titer secondary antibodies and 2 ECL reagents, in two quizzes, consisting of questions about antibodies and the Western blot technique. Each participant of the workshop received a pdf of the presentation with links to additional information. The recording of the workshop is available on demand. If you are interested in this type of online workshop to be held at your institution, please contact us! | ![]() Feedback from one of the participants: "Thank you very much for the seminar, I think was very interesting and enjoyable." |
Sample: Maize protoplasts fixed in 4% paraformaldehyde in 1X PBS pH 7.4 for 1 hour and washed 3 times in 1XPBS buffer Primary antibodies: Agrisera AS21 4615 actin monoclonal; clone 14H4G8; Secondary antibodies: Agrisera Donkey anti-Mouse IgG (H&L), DyLight® 488 conjugated AS10 1201 Other reagents: 30% Bovine Serum Albumin solution, Sigma Aldrich Cat # A8577-50ML; Phosphate Buffered Saline 10X, Electron Microscopy Sciences Cat # 19342-10; Imaging spacer, Millipore Sigma Grace Bio-Labs SecureSeal™ imaging spacer Cat # GBL654008 Protocol:
Courtesy of Dr. Anastasiya Klebanovych, Dr. Kirk Czymmek, Dr. Kevin Cox, Dr. Blake Meyers, at the Donald Danforth Plant Science Center, USA | ![]() |
Agrisera supported the 5th International Plant Proteomics Organization (INPPO) conference, held between the 22nd and 25th of May 2023, in Thessaloniki, Greece. The conference focused on basic and applied plant proteomics research.
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The conference Straub Days 2023, took place between 25th and 26th of May at the Biological Research Centre, Szeged, Hungary. During the conference, the poster "Plant cell immunolocalization and the use of advanced imaging techniques at HCEMM Advanced Core Facility" was presented by Dr. Ferhan Ayaydin and Dr. Divya Teja Dondapati from the Functional Cell Biology and Immunology-Advanced Core Facility, HCEMM, Szeged; and Institute of Plant Biology, BRC, Szeged. The poster showed various methods of material preparaton, and included immunolocalization results obtained with Agrisera antibodies to: These were combined with matching Agrisera secondary antibodies:
The plant material used were: Oryza sativa L. ssp. japonica cv. 'Unggi 9', Zea mays hybrid variety, Arabidopsis thaliana expressing GFP, and Oryza sativa bpt-jeelakarra variety. Sample preparation methods followed for the immunolocalization studies on dicot and monocots were: suspension culture, root squash, whole root mount and cryosectioning. | ![]() Seen in the image, left to right: Dr. Ferhan Ayaydin and Dr. Divya Teja Dondapati. |
Protein Extraction Buffer (PEB) is an extraction buffer for the disruption and solubilization of total protein from plant tissues and algal cells. The buffer contains the anionic detergent LDS, which does not form crystals while stored at 4-10°C, and is quickly ready to use. Expected yields will be 1.5-6 µg/µl total protein (recovered from standard procedure), depending on the starting material, e.g. its biological stage and homogenization method used (bead beater vs. sonication). For processing of multiple samples, simultaneous extraction using for example a bead beater is recommended, for consistency of extraction. Provided as 5 x 2 ml (4x stock), Agrisera's PEB buffer allows up to 75 isolations of plant material (using 500 μl 1x PEB for 100 mg fresh weight), or 190 isolations of algal material (using 200 μl 1x PEB for cell amounts corresponding to 4-10 μg total chlorophyll). To product page: PEB (4x) | protein extraction buffer | Chosen references: • Altuntas et al. (2020). Proline-stimulated signaling primarily targets the chlorophyll degradation pathway and photosynthesis associated processes to cope with short-term water deficit in maize. Photosynth Res. 2020 Apr;144(1):35-48. doi: 10.1007/s11120-020-00727-w. • Pérez-López et al. (2020). Transcriptome Analysis Identifies Plasmodiophora brassicae Secondary Infection Effector Candidates. J Eukaryot Microbiol. 2020 Jan 11. doi: 10.1111/jeu.12784. • Morin et al. (2019). Morin et al. (2019). Response of the sea-ice diatom Fragilariopsis cylindrus to simulated polar night darkness and return to light. Limnology and Oceanography. 9999, 2019, 1â??20. (sea-ice diatom) • Bausch, A.R., Juhl, A.R., Donaher, N.A. et al. Mar Biol (2019) 166: 80. |
On the 26th of May, Dr. Joanna Porankiewicz Asplund, Technical Support Manager at Agrisera, conducted a Western blot training for the sales force of Agrisera's distributor in South Africa, Bicom Biotech. The aim was to show which are the most crucial steps of Western blot, and how to help customers using this widely employed protein analysis technique. Good luck with helping your customers succeed with their Western blots! | ![]() |
As climate change becomes more noticeable, and affect all life on Earth, research on how plants respond to environmental stress intensifies in many laboratories worldwide. Agrisera antibodies, to key proteins involved in plant stress response, help solve many pressing questions of how plants tackle changes to, and threats in, their environment. New antibodies are added continuously to our collection, which includes antibodies to proteins involved in: | ![]() |
On the 15th of May, Dr. Joanna Porankiewicz-Asplund, Agrisera's Technical Support Manager, conducted an online workshop for researchers from the Wageningen University & Research Laboratory of Biochemistry, The Netherlands. The title of the workshop was "Western blot - a technique possible to optimize". The workshop was almost two hours long, including a 10 minutes break for the Agrisera Antibody and Western blot Quizzes and coffee. Questions submitted by attendees were answered, and many pitfalls of the Western blot technique were discussed, with specific experimental examples. Participants also had a chance to win Agrisera high titer secondary antibodies and 2 ECL reagents, in two quizzes, consisting of questions about antibodies and the Western blot technique. Each participant of the workshop received a pdf of the presentation with links to additional information, as well as a pdf of Agrisera's Western blot guide. The recording of the workshop is available on demand. If you are interested in this type of online workshop to be held at your institution, please contact us! | ![]() Feedback from one of the participants: "Thanks! It was a very nice and informative workshop :)" |
Beside 50 µg of antigen-purified antibodies, we will also provide you with:
- High-titer, matching secondary antibodies, to be used at 1: 25 000, 1h/RT incubation
- Agrisera ECL reagents of mid-picogram or extreme low femtogram detection range
- Suggested protocol
Product number | Product name | Uniprot ID | Research area |
AS21 4612 | BAR | Phosphinothricin N-acetyltransferase (170-183) | P16426 | Epitope tags |
AS21 4613 | BAR | Phosphinothricin N-acetyltransferase (36-50) | P16426 | Epitope tags |
AS22 4832 | Ble tag | Bleomycin resistance protein | P17493 | Epitope tags |
AS16 3690 | Cas9 | CRISPR-associated endonuclease 9 (polyclonal) | Q03JI6 | Epitope tags |
AS22 4855 | CBD-Tag | Chitin Binding Domain Epitope Tag | Epitope tags | |
AS21 4686 | CBP epitope tag | Epitope tags | |
AS22 4854 | CBP-tag | Calmodulin Binding Protein Eptiope Tag | Epitope tags | |
AS21 4604 | E Tag | Epitope tags | |
AS22 4800 | GFP11 | C-terminal of GFP | P42212 | Epitope tags |
AS21 4607 | Glu-Glu Tag | Epitope tags | |
AS21 4605 | Halo Tag | Epitope tags | |
AS21 4603 | HSV epitope tag | Epitope tags | |
AS21 4681 | HSV epitope tag | Epitope tags | |
AS21 4680 | KT3 epitope tag | Epitope tags | |
AS21 4675 | mStrawberry | Epitope tags | |
AS21 4602 | Rho1D4/1D4 Tag | Epitope tag | Epitope tags |
AS21 4684 | S epitope tag | does not apply | Epitope tags |
AS21 4606 | SNAP/Clip Tag | Epitope tags | |
AS21 4508 | Trx Tag | Thioredoxin 1 Fusion protein | Epitope tags |
Contact us with your antibody choice!
Agrisera supported the 32nd Western Photosynthesis Conference, held between the 28th and 30th of April 2023, in Bodega Biological Marine Laboratory, California, USA.
Dr. Asmit Bhowmick from the Lawrence Berkeley National Laboratory, and Grad student Albert Liu from University of California Davis. Awaiting your free antibody choices! | ![]() |
For your convenience, chosen Agrisera antibodies can be purchased as sets at very favorabe pricing. The sets also include high-titer matching secondary antibodies, which can be used at 1: 25 000 1h/RT incubation, and Agrisera's ECL reagents, which offer versatile detection. Educational antibody set for photosynthesis | Photosynthesis Tool kit – quantitation (includes standards for quantification of PSI and PSII) | Oxidative stress antibody set | Argonaute antibody set | Rubisco quantitation set (for Western blot or ELISA) | Plant Cell Compartment Antibody Marker Set for 3, 5 or 10 marker antibodies of your choice. You are always welcome to suggest a new antibody set to us! | ![]() An example of Agrisera Antibody Set, including high-titer matching secondary antibodies and ECL reagent. |
Agrisera supported the ASPB Northeastern Section 2023 Meeting, held between the 22nd and 23rd of April, 2023, in SUNY Old Westbury, USA. The meeting was organized ny Prof. Christos Noutsos. The winners of Agrisera Best Poster Prizes, and a free antibody each from the Agrisera catalog, were: Cassandra Probert from Samuel Hazen’s Lab at the University of Massachusetts, with the poster "BdSND2R is a transcriptional repressor that forms a negative feedback loop with SWAM1 to control secondary wall thickening in Brachypodium distachyon" and Alaa Mohamed Elsaeed Hassan Elminisy from Om Prakash Dhankher lab at the University Massachusetts Amherst, with the poster "Molecular and Biochemical studies on the interaction between cucumber and Plant Growth Promoting Rhizobacteria (PGPR) under Cadmium (Cd) Stress". We are awaiting your free antibody choice! | ![]() Left to right: Cassandra Probert, Professor Christos Noutsos and Alaa M. El-Minis. |
Antibodies to Arabidopsis thaliana proteins involved in photosynthesis are a core collection of the Agrisera catalog. This group of products has been developed in collaboration with research laboratories worldwide, for the last 20 years. Agrisera photosynthetic antibodies have the following features:
Beside antibodies, Agrisera has also developed Educational Posters on Photosynthesis, that are engaging and packed with up-to-date information. These posters are available for download, and as hard copies that can be requested here. Each poster was created in consultation with leading researchers from the scientific community, and the information in the posters is updated regularly. Specific figures and schemes from the posters are available free of charge for download, to be used in publications and presentations. | ![]() |
Agrisera supported the 24th conference of the Austrian Society of Plant Biology (ATSPB), held between the 13th and 15th of April, 2023, in Hall in Tirol, Austria. The event was organized by the Department of Botany of the University of Innsbruck. The 24th conference of the ATSPB was dedicated to the importance of plants in the context of food security, biodiversity and climate change. The Agrisera Best Poster Prize was awarded to Paraskevi Charalambous, from the University of Natural Resources and Life Sciences, Vienna (BOKU), Austria, for the poster "Dissolving and Reshaping Walnut Shells: A Sustainable Material Solution?". Awaiting your free antibody choice! | ![]() In the picture: Paraskevi Charalambous (left) and Prof Ilse Kranner, Conference President (right). |
Anti-PsbO antibodies, product number: AS06 142-33, detect a PsbO protein in a wide range of different species. Recently, reactivity on a new species, Eucalyptus grandis, has been experimentally confirmed using the following protocol: 13.5 µg/well of total protein extracted freshly from Eucalyptus sp. leaves. Exact buffer components were: 50 mM HEPES NaOH pH 8.0, 5mM DTT, 15 mM NaHCO3, 20 mM MgCl2, 2 mM EDTA, 4% (v/v) EDTA- free protease inhibitor cocktail (Roche Diagnostics), 4% PVPP (w/v), 20% glycerol (v/v), 1:1 Tris-buffered phenol (v/v) and 100 mM CH3COONa in methanol. Extracts were denatured with SDS beta-Mercaptoethanol sample buffer for 10 min at 90°C. Samples were separated at RT on 12% SDS-PAGE and blotted for 1 h to PVDF, using wet transfer at room temperature (RT). Blot was blocked with 5% non-fat milk for 1 h at RT with agitation. Blot was incubated in the primary antibody at a dilution of 1:5000 for 1 h at RT with agitation in TBS-T. The antibody solution was decanted, and the blot was rinsed briefly twice, then washed three times for 10 min with TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG HRP, Agrisera AS09 602) diluted to 1: 10000 in TBS-T for 1 h at RT with agitation. The blot was washed as above and developed with a following chemiluminescent detection reagent. Exposure time was 12 min. | ![]() Samples: 1: 13.5 µg of Eucalyptus grandis whole leaf extract clone one (g1) |
With millions of antibodies offered worldwide, by hundreds of suppliers, it may be a bit overwhelming, especially for beginners, to choose the correct antibody to answer a specific questions in a research project. Here is a short checklist with what to pay attention to: Species reactivity – Has the antibody been used to detect the protein from the species we are working with? If not, a request should be sent to the supplier to confirm if the antibody may have so-called predicted reactivity to the target protein. If not, the antibody is not suitable, and false positive results can be produced. Validation data – Is validation data available on a product information sheet? For non-essential proteins, validation data should include results obtained with knockdown or knockout mutants. Western blot results showing that an antibody binds to a recombinant protein, are not relevant to the binding in a specific sample, extracted from a specific tissue or organism. Here we explain why. Recommended antibody dilution – The lower the dilution, the fewer tests can be conducted using an antibody. Here we explain why. Publication record – Being citet in scientific articles definitely confirms the performance of a given antibody. However, as there is no worldwide standard, product numbers are oftentimes not included in the "Material and Method" section. This makes it difficult to identify what antibody has been used to obtain the presented results. How to cite antibodies in scientific publication, is explained in this short video. | ![]() You are always welcome to contact us with any questions, and we will promplty help you to find the best antibody for your specific species and application. |
The 19th International Workshop on Plant Membrane Biology (IWPMB) took place Taipei, Taiwan, between 27th and 31st of March. This meeting has been organized since 1968 and covers topics related to membrane transport in the context of signaling, physiology, biotic and abiotic stresses and development. Agrisera supported the meeting with two Best Poster Prizes. The Best Poster Prizes were awarded for the scientific poster contribution of Huimin Feng from Nanjing Agricultural University, the poster title was "P02-11 Strigolactone and gibberellin signalling coordinately regulates metabolic adaptations to changes in nitrogen availability", and Dr. Swee-Suak Ko from Academia Sinica, Biotechnology Center in Southern Taiwan with the poster "P11- 11 Redefine the Role of OsPHO1;2 Phosphate Transporter in Rice Grain Filling". We are awaring your free antibody choices! Good luck with your research! | |
Well-validated antibodies to specific cellular compartments are crucial to support data regarding localization studies of novel proteins. Agrisera's Cellular compartment antibody collection contains of marker antibodies to: Agrisera compartment marker antibodies have been used in in a wide range of publications over the last 15 years. For many compartments we offer several antibodies, to proteins of different molecular weight, to match your experimental requirements. Not certain which compartment marker antibody to choose? Ask Agrisera's techincal staff for an advice. | ![]() |
Our warmest congratulations to master student Karolina Todorska, who scored a 9/9 in the Agrisera Antibody and Western blot quiz, part 1 and part 2! The quiz was a part of Agrisera's lecture on Western blot, conducted by Dr. Joanna Porankiewicz Asplund on the 29th of March, as part of the course "Biochemistry: Protein Separation Methods and Analytical Techniques", held at The Department of Chemistry, Umeå University, Sweden. As the prize, Karolina received the book "Photosynthesis – Solar Energy for Life" by Dr. Dmitry Shevela, Prof. Lars Olof Björn, Prof. Govindjee. Good luck with your studies! | ![]() |
Agrisera offers an extensive and well-published collection of antibodies to support research on the biology of the model plant Arabidopsis thaliana.
Find a matching antibody to a gene ID | Complete list of antibodies with confirmed and predicted reactivity to Arabidopsis thaliana | Examples of publications with Agrisera Antibodies | Benefit from a 10% discount until end of June with the discount code Arabi10 | Marker antibodies for Arabidopsis thaliana cellular compartments Extensive collection of antibodies to Arabidopsis thaliana proteins involved in photosynthesis Extensive collection of antibodies to Arabidopsis thaliana proteins involved in a stress response ![]() Agrisera's Arabidopsis thaliana antibodies are suitable for a wide range of techniques, including immunofluorescence, ChIP, IP and Western Blot. Over 100 antibodies are available free of charge for testing, in return for results. Check the list here. |
On the 28th of March, Agrisera participated in a Job Fair at Umeå University. The event is organized annually by Umeå Biotech Incubator, with the aim to connect students and potential new work places within the Life Science sector in Umeå. A warm thank you to all the students who came by and asked us interesting questions. We do hope that you see Agrisera as an attractive future workplace! | ![]() Agrisera Laboratory Technician Marcus Boman and Product Developer Evelina Höök represented Agrisera at the Job Fair. |
On the 24th of March, Dr. Joanna Porankiewicz-Asplund, Agrisera's Technical Support Manager, conducted an online workshop for researchers from the Max Planck Institute of Molecular Plant Physiology in Potsdam-Golm, Germany. The title of the workshop was "Western blot - a technique possible to optimize". The workshop was almost 2 hours long, including a 10 minutes break for the Agrisera Antibody and Western blot Quizzes and coffee. Questions submitted by attendees were answered, and many pitfalls of the Western blot technique were discussed, with specific experimental examples. Participants also had a chance to win Agrisera high titer secondary antibodies and 2 ECL reagents, in two quizzes, consisting of questions about antibodies and the Western blot technique. Each participant of the workshop received a pdf of the presentation with links to additional information, as well as a pdf of Agrisera's Western blot guide. If you are interested in this type of online workshop to be held at your institution, do not hesitate to contact us! | ![]() Feedback from one of the participants: Thank you very much for the workshop! I myself learned quite some additional things, I didn’t know before. And I talked to some of my colleagues just now and they also really liked your workshop. Thanks also for providing the pdf, I will send them to the participants! I really enjoyed your presentation and I can see how dedicated you are about western blots, it was really great to listen to you! |
Agrisera offers a wide range of high-titer secondary antibodies, for detection of primary antibodies from different hosts, like goat, llama, mouse, rabbit, rat and many more. Our secondary antibodies have the following features:
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