Agrisera blog

Jan Andersson Award of the Australian Society of Plant Scientists, supported by Agrisera has been awarded to Assoc Prof Jenny Mortimer

This year's Jan Anderson Award, granted by the Australian Society of Plant Scientists was awarded to Associate Prof. Jenny Mortimer from School of Agriculture, Food and Wine of the University of Adelaide, Australia. Her group uses synthetic biology to develop new crops for food, and materials production, in controlled growth environments. This award acknowledges talented female plant science researchers, and is sponsored by Agrisera.

 

   Australian Society of Plant Scientists
Read more 2024-09-23

Protein Extraction: Frist Step to Successful Downstream Application, Agrisera Educational Poster 8

Agrisera's Educational Poster number 8 is the first poster in our collection that focuses on methods of protein extraction. The content was developed in a collaboration between Agrisera's Technical Support Manager Dr. Joanna Porankiewicz-Asplund, and researchers with a long experience with protein extraction; Dr. André Graça, Prof. Antje Heese, Prof. Martha Ludwig and prof. Wolfgang Schröder. The contents were transferred into the graphical representation by Dr. Dmitry Shevela (SciGrafik, Sweden).


You are welcome to download the pdf or request a hard copy of this very informative poster! The poster also contains a link and QR code to a collection of protocols and references, for protein extraction methods from various plant species, organs and cellular compartments.

If you have any comments or suggestions, we are always eager to hear them. You are welcome to contact us!

  Protein extraction - Agrisera Educational Poster 8

 

Read more 2024-09-18

Agrisera at the conference of the German Society of Plant Sciences

Agrisera participated in the International Conference of the German Society for Plant Sciences (DBG). The event took place at the Martin Luther University Halle-Wittenberg, Germany, between the 15th and 19th of September. Hundreds of enthusiastic plant scientists attended the meeting, which covered a wide range of topics, including biotechnology, cell biology, communication and dynamics of plant organelles, navigating abiotic challenges, and plant development, to name a few. 

Agrisera's 8th Educational Poster, focused on Protein Extraction, received a lot of attention. As did our other posters, on the topics of photosynthesis and epigenetics. 

There were many interesting discussions, suggestions and questions from visitors to Agrisera's booth, which we are very grateful for. We are always looking forward to meeting end users of Agrisera antibodies, as well as those who are considering future antibody work. Agrisera supports your research, not only with suitable products, but also technical support and troubleshooting.

Agrisera had a fortune wheel at our booth, which visitors could spin to receive help with common questions related to plant science and antibody techniques. Doing this, the participant also had a chance to win a free antibody of their choice from the Agrisera catalog. The fortune wheel was busy, and the winners of free antibodies will be revealed at the end of the week.
  
  

 

   Agrisera at the German Society of Plant Sciences conference in Halle

Read more 2024-09-17

Agrisera at Plant Meiosis meeting

Agrisera participated in the Plant Meiosis meeting, held between the 4th and 6th of September, at Adam Mickiewicz University in Poznań, Poland. The meeting gathered enthusiastic researchers from the plant meiosis community, and was packed with interesting talks and poster sessions. 
  
Two Agrisera's Best Poster Prizes were awarded to: Hasibe Tuncay from Universität Hamburg in Germany, for the poster: "DREAMing Meiosis: Transcriptional Control of Male Meiosis by DREAM-complex in Arabidopsis thaliana" and Côme Emmenecker from Université Paris-Saclay, INRAE for the poster "Multiple factors counteract FIGL1 activity to promote meiotic
DSB repair".

Agrisera had a fortune wheel at our booth, which visitors could spin to receive help with common questions related to plant science and antibody techniques. Doing this, the participant also had a chance to win a free antibody of their choice from the Agrisera catalog. This activity was very popular, and a queue was even formed of people looking to try their luck. 

Thank you for very interesting interactions!

 

 Agrisera at Plant Meiosis Meeting

Read more 2024-09-06

Agrisera at Plant Energy Management meeting in Umeć

Agrisera participated in the Plant energy management, molecular mechanisms and signalling conference, held between 27th and 30th of August at Umeå University, Sweden. Over the last 40 years, Agrisera has developed strong collaborations with researchers from this local university, and we are always eager to support and participate in the local conferences. 
 
Agrisera's Best Poster Prize was awarded to Maroof Ahmed Shaikh from the group Light and temperature control of plant development at the Center for Research in Agricultural Genomics (CRAG), Spain. His poster title was:

"Unleashing the Light Dependent Role of GIGANTEA : Orchestrating Tuberization and Meristem Activity in Potatoes".

As the winner, he will be granted a free antibody of his choice from the Agrisera catalog.

Good luck with your research, and we are awaiting your fee antibody choice!



Picture to the right: Agrisera's table at the conference, and Awardee Maroof Ahmed Shaikh together with conference chairman Prof. Johannes Hansson. 

  


 

 Awardee of Agrisera Best Poster Prize during Plant Energy Managment Conference in Umeå

Read more 2024-09-04

Visit to Agrisera facility in Umeć

Participants of the Plant energy management, molecular mechanisms and signalling conference, visited Agrisera's facility in Umeå. Principal Investigators, PostDocs and PhD students joined the tour on the 28th of August. Our visitors were from Columbia, India, France, Greece, Germany, Korea, Spain and Sweden.
 
Many interesting questions were asked regarding antibody production, purification and validation. 

We eagerly shared our knowledge and are hoping for future collaborations!

  


 

Visit to Agrisera facility during Plant energy managment conference
After a short presentation of Agrisera's purification and QC lab, the guests also got a peak into the packing room and offices. 

Read more 2024-08-28

Meet Agrisera during coming conferences

We are keen on meeting you!

If you are attending any of the coming meetings, you are very welcome to come by Agrisera's booth to talk antibodies, or to pick up an educational poster or a Western blot troubleshooting guide. Agrisera will be present at the following upcoming meetings:

Plant energy management, molecular mechanisms and signalling, August 26th-28th, Umeå University, Sweden

Plant Meiosis Meeting, September 4th-6th, Poznań, Poland

International Conference of the German Society of Plant Sciences, September 15th-19th, Halle, Germany



 

Meet Agrisera on conferences during autumn 2024

Read more 2024-08-23

10 % discount on any Agrisera product in return for a short review

Is the product you are interested in not included in a current promotion?

We offer a 10% discount in return for a short review, left on a the product page of an item that you, or a colleague of yours, have previously used in the lab. 

How to leave a short review: 
• Go to the product page of the item you would like to review.
• Click on the "Reviews" tab.
• Click on "Leave a review".
• Write the name of a species that the analyzed sample came from, and in which application the product was used. Shortly describe the obtained result.
• Post the review.
• Contact your distributor or [email protected] to obtain a discount code or a discounted offer for your next purchase.

 

Leave a review of Agrisera product and receive a 10 % discount

Read more 2024-08-16

Four new species added to Search by species page

Four new species have been added to Agrisera's Search by species function, namely:

Camelina sativa
Colobanthus quitensis
Panicum sp.
Raphanus sativus

This resource now lists 40 different species, ranging from higher plants, to mosses, algae and cyanobacteria, and offers a quick and easy way to find antibodies reactive in your species of interest.

Remember that you can also filter the lists of primary antibodies, in any product category on our website, by reactivity, applications and clonality.

 

Read more 2024-08-07

Use anti-BirA (mutated/TurboID) antibodies for identification of protein-protein interactions

Tagging a protein with TurboID allows the study of protein interactions in different types of cells and organs, as well as different developmental stages. This is a suitable tool for proximity labelling experiments, as described in Mair et al. (2019). Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife. 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864.

The product information sheet for Agrisera's anti-BirA (mutated/TurboID) antibodies can be found here

 

References with Agrisera's BirA (mutated/TurboID):

Liu et al. (2024). Activation of kappa opioid receptor suppresses post-traumatic osteoarthritis via sequestering STAT3 on the plasma membrane. Cell Commun Signal. 2024 Jun 18;22(1):335.doi: 10.1186/s12964-024-01709-4.  
Medica et al. (2023). Proximity-dependent mapping of the HCMV US28 interactome identifies RhoGEF signaling as a requirement for efficient viral reactivation. PLoS Pathog. 2023 Oct 2;19(10):e1011682.doi: 10.1371/journal.ppat.1011682.
Shi et al. (2023). Protocol to identify protein-protein interaction networks in Solanum tuberosum using transient TurboID-based proximity labeling. STAR Protoc. 2023 Sep 20;4(4):102577.doi: 10.1016/j.xpro.2023.102577.

Read more 2024-08-02

Use the Global Plant Events Calendar to check for coming events and workshops

The Global Plant Events Calendar, run by ASPB/Plantae and Agrisera has celebrated its fifth year! This resource provides you with a comprehensive list of events, like conferences, workshops and webinars, some being free of charge. 

The list for 2025 events keeps filling up, to aid your conference attendance planning.

If you are organizing a meeting, you are welcome to sign it up into the calendar, which will make it visible worldwide, through this very popular resource. All events displayed in the calendar are curated, to protect you from irrelevant information and predatory conferences. 



 


Global Plant Events Calendar July 2024

Read more 2024-07-28

Free epitope tag antibodies

Beyond the current 15% discount for all epitope tag antibodies, Agrisera offers several epitope tag antibodies, currently under development, free of charge, in return for experimental results.
The offer includes: 

• 50 µg of antigen affinity purified epitope tag antibody
• High-titer matching secondary antibody
• Agrisera chemiluminescent detection reagent

Take advantage of this unique offer and review a complete list here.

Let us know which antibodies you would like to test!



 

Agrisera Free Epitope Tag Antibodies

Read more 2024-07-26

Agrisera at ICAR 2024, San Diego, USA

Agrisera attended the 34th International Conference on Arabidopsis Research, in SanDiego USA, held between the 15th and 19th of July. Agrisera was represented by Dr. Chris Brown.

Thank you to everyone who visited Agrisera's booth to discuss antibodies and plant science, and to the conference organizers for yet another successful meeting. 

Agrisera's Educational Posters were available free of charge at the conference. In case you did not get a chance to obtain copies, you can request them, free of charge, here.


 


Agrisera at ICAR 2024

Read more 2024-07-19

Two conferences supported this week by Agrisera

The International Symposium on Iron Nutrition and Interactions in Plants (ISINIP) took place between the 8th and 12th of July, in Düsseldorf in Germany. Scientific sessions covered the following topics:

  • Crop iron physiology 
  • Micronutrient biofortification 
  • Plant-soil-microbe interactions 
  • Alkaline and acidic soil environments 
  • Transcription factor networks 
  • Iron acquisition and transport 
  • Whole-plant iron physiology and regulation 
  • Iron physiology and interaction with other elements 

Best Poster Prizes from Agrisera were awarded to two young scientists. 


The Plant Epigenetics meeting took place between 10th and 12th of July, in Clermond-Ferrand in France.

Agrisera supported this meeting with hard copies of our educational posters focused on epigenetics, which were developed in collaboration with scientists in the field. 
 

 
 

 

Two conferences supported by Agrisera this week

Read more 2024-07-13

Do not miss: 15 % off all antibodies to rice proteins

Rice is a staple food for more than half of the world's population, particularly in Asia, Africa, and Latin America. Research on this important crop includes:

• Nutritional Improvement like Golden Rice with increased vitamin A.
• Investigation of rice climate change adaptation by development of varieties which are more tolerant to drought, salinity, and temperature extremes as well as flooding.
• Research on pests and diseases, which can help reduce the use of chemicals and lead to more sustainable agriculture.
• Understanding of genetic diversity and preservation of wild rice species, which are valuable genetic resources for future breeding programs.

All Agrisera antibodies to rice proteins are offered with a 15% discount between July and September of this year. Browse Agrisera rice antibody collection here.

To apply the discount, use discount code Rice15 at checkout, or when placing your order by email.
 

 

Agrisera antibodies to rice proteins 15 % off

Read more 2024-07-04

Agrisera supported Young Researchers Symposium on Plant Photobiology

The Young Researchers Symposium on Photobiology (YRSPP) took place between 2nd and 5th of July in Utrecht, The Netherlands. 

Agrisera's Best Talk prize was awarded to Johannes Rötzer, from the Dolan Group at the GMI Vienna, part of the Vienna BioCenter for the talk "The role of blue light receptors during cell polarization in Marchantia polymorpha".

Congratulations!

Agrisera supported the meeting with hard copies of our educational posters focused on photosynthesis, which were available to participants. 

Agrisera Educational Posters developed in collaboration with scientists in the field of photosynthesis.


 

 

Yound Researchers Symposium on Plant Photobiology

Read more 2024-07-02

Agrisera supported Second European Congress on Photosynthesis

The Second European Congress on Photosynthesis (EPS2) took place between the 25th and 28th of June in Padova, Italy.

Agrisera supported the meeting with hard copies of our educational posters focused on photosynthesis, which were available to participants.

Agrisera's Educational Posters were developed by Dr. Dmitry Shevela, Prof. Govindjee, and other leading scientists in the covered fields. More information on the authors can be found below. Graphic design by Dmitry Shevela (SciGrafik).
 

 

EPS2 Padova, Italy  2024

Read more 2024-06-29

Do not miss: 15 % off all epitope tag antibodies until end of 2024

Epitope tag antibodies offer several benefits that make them invaluable tools for studying proteins in a wide range of applications, like Western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, and flow cytometry.

The most commonly used epitope tags are:
HIS (3X or 6X), FLAG, HA, Myc, GFP and its variants.

Agrisera offers antibodies to a wide range of common and less commonly used epitope tags, for example:

HIS | FLAG | HA | Myc | GFP
 
Until end of December, all Agrisera epitope tag antibodies are offered with a 15% discount.

Use the discount code Tag15.
 

 

Agrisera epitope tag antibodies 15 % off
To browse all Agrisera epitope tag antibodies, check here.

Read more 2024-06-28

Agrisera at Plant Biology 2024, in Honolulu, Hawaii

Agrisera attended the Plant Biology 2024 conference in Honolulu, HI, USA, held between the 22nd and 26th of June. Agrisera was represented by Edith Kalén and Chris Brown.

Thank you to everyone who visited Agrisera's booth to discuss antibodies and plant science, and to the conference organizers for yet another successful meeting. Our warmest congratulations to ASPB on their 100th anniversary!

Agrisera's Educational Posters were available free of charge at the conference. In case you did not get a chance to obtain copies, you can request them, free of charge, here.

Hope to see you in Milwaukee next year!

 


Top: Agrisera's booth at Plant Biology 2024
Bottom, left to right: Conference opening reception, ASPB's 100th anniversary party.

Read more 2024-06-27

Confirm fraction purity with Agrisera compartment marker antibodies

Nguyen et al. used Agrisera antibodies to α–phosphoenolpyruvate carboxylase (PEPC) and histone 3 (H3) in the recent publication "The processed C-terminus of AvrRps4 effector suppresses plant immunity via targeting multiple WRKYs ", published in Journal of Integrative Plant Biology, June 2024.

The following antibodies were used to confirm the cytoplasmic and nuclear fraction's purity:

Anti-PEPC (AS09 458) (cytoplasmic fraction)

Anti-H3 (AS10 710) (nucelar fraction)



 



    Agrisera antibodies used to confirm fraction purity

Read more 2024-06-22

Meet with Agrisera at Plant Biology 2024 in Honolulu


Between June 22nd - 26th, Agrisera is going to be present at the Plant Biology 2024, Honolulu, Hawai. The conference is organized each year by American Society of Plant Biologists (ASPB). The theme of this year's meeting is: "Celebrating Our Past through Science for Our Future".

Sc.Eng. Edith Björnberg Kalén and Dr. Chris Brown are representing Agrisera at the meeting. You are welcome to come by Agrisera booth and talk antibodies with them.  Dr. Brown has been involved in the development of Agrisera's Global Antibodies, to a wide range of key photosynthetic proteins, with broad species reactivity. 

Participants can pick up free hard copies of Agrisera's Educational Posters, prepared in collaboration with scientists in the field of photosynthesis and epigenetics, as well as the Agrisera product catalog and Agrisera Western blot troubleshooting guide

 

    Plant Biology 2024

Come by and meet with Agrisera!
 

Read more 2024-06-20

Multiple Agrisera photosynthesis antibodies applied for a novel mutant characterization

Krysiak et al. used several of Agrisera antibodies to photosynthetic proteins in the recent publication "Light-independent pathway of STN7 kinase activation under low temperature stress in runner bean (Phaseolus coccineus L.)", published in BMC Plant Biology, June 2024.

The following antibodies were used in mutant characterization:

Anti-AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit antibodies), AS05 085
Anti-CURT1A | Curvature thylakoid 1A, AS08 316
Anti-Lhca2 | PSI type II chloropyll a/b-binding protein, AS01 006
Anti-Lhcb1 | LHCII type I chlorophyll a/b-binding protein, AS01 004
Anti-Lhcb1-P | LHCII type I chlorophyll a/b-binding protein, phopshorylated, AS13 2704
Anti-Lhcb2 | LHCII type II chlorophyll a/b-binding protein, AS01 003
Anti-Lhcb2-P | LHCII type II chlorophyll a/b-binding protein, phosphorylated, AS13 2705
Anti-NtrC | Thioredoxin reductase (TR/TRxR) (higher plants), AS07 243
Anti-PsaA | PSI-A core protein of photosystem I, AS06 172
Anti-PsaB | PSI-B core subunit of photosystem I, AS10 695
Anti-PsbA | D1 protein of PSII, DE-loop, AS10 704
Anti-PsbC | CP43 protein of PSII, AS11 1787
Anti-RbcL| Rubisco large subunit, form I (rabbit), AS03 037



 



   Mutant characterization using Agrisera antibodies

Read more 2024-06-16

Agrisera antibodies to photosynthetic proteins support research on how to improve crop yields

Zhao et al. used several of Agrisera antibodies to photosynthetic proteins in the recent publication "Psb28 protein is indispensable for stable accumulation of PSII core complexes in Arabidopsis",  published in Plant Journal, May 2024.

The following antibodies were used in mutant characterization:

Anti-AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial), AS05 085
Anti-Cyt f | Cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex, AS08 306 (AS20 4377)
Anti-Deg8 | protease Do-like 8 (chloroplastic), AS14 2767
Anti-FtsH1+FtsH5 | ATP-dependent zinc metalloprotease FtsH1+FtsH5 (chloroplastic), AS16 3930
Anti-FtsH2+FtsH8 | ATP-dependent zinc metalloprotease FtsH2+FtsH8 (chloroplastic), AS16 3929
Anti-Lhcb2 | LHCII type II chlorophyll a/b-binding protein, AS01 003
Anti-Lhca2 | PSI type II chloropyll a/b-binding protein, AS01 006
Anti-PsaA | PSI-A core protein of photosystem I, AS06 172
Anti-PsbA | D1 protein of PSII, C-terminal, AS05 084
Anti-PsbB | CP47 protein of PSII, AS04 038
Anti-PsbC | CP43 protein of PSII, AS11 1787
Anti-PsbD | D2 protein of PSII, AS06 146
Anti-PsbE | Alfa subunit of Cytochrome b559 of PSII, AS06 112
Anti-PsbF | beta subunit of Cytochrome b559 of PSII, AS06 113
Anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-peptide), AS05 092
Anti-RbcL | Rubisco large subunit, form I, AS03 037





    Agrisera antibodies upport research on photosynthesis

Read more 2024-06-08

Agrisera supports two conferences this week

This week, Agrisera supported two conferences:

International Society of Root Research, 12th International Symposium, June 2nd-7th, Leipzig, Germany. The three most important global challenges in terms of root biology are:  climate change, biodiversity loss, and food security. Root research has a fundamental role in helping to solve these problems.

Postdoc Day 2024, 6th of June, UNIGE, Switzerland is organized each year by UNIGE PostDocs. Agrisera supported the meeting with The Best Talk Prize and the Agrisera Educational Posters


    Agrisera plant conference support June 2024

Read more 2024-06-07

Meet with Agrisera at 2nd American Photosynthesis Congress


Agrisera is present at the 2nd American Photosynthesis Congress, held June 3rd-6th, at Georgia State University, USA. The conference covers all aspects of photosynthesis research. 

Participants can pick up free hard copies of Agrisera's Educational Posters, prepared in collaboration with scientists in the field of photosynthesis, as well as the Agrisera product catalog and Agrisera Western blot troubleshooting guide

Dr. Chris Brown is representing Agrisera at the congress. Come by and ask him any questions about antibodies for photosynthesis research. Dr. Brown has been involved in the development of Agrisera's Global Antibodies, to a wide range of key photosynthetic proteins, with broad species reactivity.

   Agrisera at 2nd American Photosynthesis Congress
Dr. Chris Brown at Agrisera's booth.

Read more 2024-06-04

Agrisera supports 13th International Coference for Plant Mitochondrial Biology


Agrisera supported the 13th International Coference for Plant Mitochondrial Biology (ICPMB), held May 26th-30th, in St Malo, France. This biennial meeting is an international forum for junior and senior academics researchers worldwide, who specialize in various aspects of plant mitochondrial biology.

Participants could pick up free hard copies of Agrisera's Educational Posters, prepared in collaboration with scientists in the covered fields, as well as the Agrisera product catalog and Agrisera Western blot troubleshooting guide



   Free image of plant mitochondria

Read more 2024-05-27

Study chloroplast biogenesis and architecture with Agrisera antibodies


Chloroplasts are essential plant cell organelles, responsible for photosynthesis and other important metabolic processes. Their biogenesis and architecture are complex and highly regulated processes. 

Agrisera antibodies can help you investigate various proteins involved in this process.
The antibody collection includes: 

CURT1A | Curvature thylakoid 1A
CURT1B | Curvature thylakoid 1B
CURT1C | Curvature thylakoid 1C
CURT1D | Curvature thylakoid 1D


You are always welcome to provide us new antibody suggestions and be the first to use the produced antibody.



  Free chloroplast image

Read more 2024-05-24

Investigate proteins involved in sucrose metabolism with Agrisera antibodies

Sucrose metabolism plays a crucial role in energy production, carbon partitioning, and sugar transport. It is therefore essential for cellular function, growth and development in plants.

Using Agrisera antibodies, proteins involved in various stages of this process can be tracked. 

Sucrose breakdown involves an enzyme called sucrase (invertase), which cleaves sucrose into the monosaccharides glucose and fructose.

Sucrose biosynthesis occurs primarily in the cytoplasm of photosynthetic cells, and involves the enzymes sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP). These enzymes are involved in the production of sucrose, which can then be transported to other plant tissues for storage or energy utilization.


Check out Agrisera's full collection of antibodies to proteins involved in sucrose metabolism

 



  Free image of maize in vegetative stage

Read more 2024-05-19

Track changes in LHC proteins using most published anti-LHC antibodies

Light-harvesting complexes (LHCs) are essential components of the photosynthetic machinery, allowing photosynthetic organisms to efficiently capture and utilize light energy for the synthesis of organic molecules. The LHCs are integral membrane protein complexes found in the thylakoid membranes of plants, algae, and cyanobacteria. 

Agrisera's LHC antibody collection has been available since the year 2001, with hundreds of scientific references for a wide range of plant and algal species. 
 
Check out Agrisera's LHC antibodies: 
Lhca1 | Lhca2 | Lhca3 | Lhca4 | Lhca5 | Lhcb1 | Lhcb2 | Lhcb3 | Lhcb4 | Lhcb5 | Lhcb6 

Full collection: Antibodies Plant/AlgalPhotosynthesisLHC
    Free image of a chloroplast

Read more 2024-05-12

Agrisera supports BioLock student conference at the University of Wrocław

 



Agrisera supports BioLock student conference, held between the 10th and 12th of May at the University of Wrocław, Poland. The aim of the meeting is, as the organizers describe it, "to expand horizons in the fields of biotechnology, biochemistry, and related disciplines. In other words, embracing both the most crucial and the latest scientific achievements!"


Agrisera supported the meeting with free hard copies of our Educational Posters.



   BioLock Student Conference

Read more 2024-05-10

Secondary Antibody Guide

Finding the correct secondary antibody can be difficult, so we have put together a Secondary Antibody Guide to help you find exactly what you need. A lot of what you need to know about Agrisera secondary antibodies can be found in their name, but it may be hard to decipher. Our guide walks you through common terms related to secondary antibodies, as well as antibody recommendations and FAQs.

Check out our guide and find answers to questions like:
  • Which secondary antibody to choose for Western blot for protein detection in plant and algal samples?
  • What is the Laser lane, Common filter set, Excitation max and Emission max for fluorescent dyes?
  • What are F(ab)'2 and Fc fragments?
  • What is the meaning of Pre-adsorbed?
And much more...

Check out Agrisera's Secondary Antibody Guide!
 

 Antibody chains
Light spectrum Dylight
Fluorescent dye table

Check out our guide for information on antibody structure, fluorescent dyes and much more!


Read more 2024-05-06

Shorten the time of Western blot or IL by using directly conjugated primary antibodies

To directly conjugated a primary antibody to an enzyme, like ALP or HRP, or a fluorescent dye, like DyLight®, the antibody must be of the highest purity; purified against the specific antigen. 

Q: What are the benefits of using directly conjugated primary antibodies?

A: The time necessary to complete all steps of a Western blot or immunolocalization protocol, is shortened considerably, as there is no need for secondary antibody incubation and subsequent washes, before the final reaction can be visualized. Potential cross-reactions, which may occur when using secondary antibodies, are also eliminated. Possible sample loss due to washing steps is also reduced. 

Until end of 2024, all directly conjugated Agrisera primary antibodies are discounted by 10%, when applying the discount code Primconj10.

Check out the complete Agrisera collection of directly conjugated primary antibodies here


  Directly conjugated primary antibodies
Using directly conjugated primary antibodies, is most beneficial for protein targets of high and moderate abundance in the analyzed tissue.

Read more 2024-05-03

20 % off for antibodies to proteins involved in electron transfer in photosynthesis

Electron transfer in the thylakoid membrane generates energy through the synthesis of adenosine triphosphate (ATP). The enzyme ATP synthase catalyzes the formation of ATP from adenosine diphosphate (ADP) and inorganic phosphate (Pi), using the proton gradient established across the membrane. Additionally, NADPH, produced during electron transfer, serves as a source of reducing power for the Calvin cycle, where carbon dioxide is fixed and converted into carbohydrates. This process in the thylakoid membrane plays a crucial role in harnessing light energy and converting it into chemical energy.

Agrisera's collection of antibodies to proteins involved in electron transfer offers you the following advantages:
  • Can be used at high dilutions
  • Broad species reactivity, including higher plants, mosses, algae, cyanobacteria and diatoms
  • Suitable for Western blot
  • Cited in hundreds of publications, over the last 20 years

Explore and pick up suitable antibodies here.
 Free scheme of thylakoid membrane
For more images of schemes and photosynthetic complexes, check free images for download here

Read more 2024-04-26

Get new tips and tricks for Western blot during Agrisera workshop on 16th of May at 11 am CET

Agrisera online Wstern blot workshop

Conducted by: Dr. Joanna Porankiewicz Asplund, Technical Support Manager from the Swedish company Agrisera
Duration: Approximately 2 hours, with Q&A sessions, and a break, during which you have the chance to win prizes in an online quiz. Western blot and antibody questions can be sent in advance to [email protected] 
Technique: Western blot is used in laboratories working with protein detection in complex mixes, as well as protein quantification.
Suitable for: Beginners and experienced users of this technique, working various types of samples, including bacterial extracts, plant tissue and algal cell cultures.
About the presenter: Dr. Joanna Porankiewicz Asplund, Technical Support Manager at Agrisera, has been working with antibody validation for over 20 years, and has troubleshooted hundreds of blots, which has allowed her to gain extensive experience, and an overview on potential difficulties and ways to address them. She is responsible for technical support at Agrisera, and will be happy to share her knowledge during the workshop.
Provided materials:
• Certificate of participation
• Slides of the presentation in pdf format, with useful links
• Slides of the presentation in ppt format, for free use in teaching
• Recording of the workshop

Sign up for this free workshop by sending an email to [email protected]!
Start: 11 am (Central European Time), 10 am (UK), 12 am (Helsinki)

Feedback from participants of previous workshops: 

"Thanks! It was a very nice and informative workshop :)" – Wageningen University & Research Laboratory of Biochemistry, The Netherlands.

"Thank you very much for the workshop! I myself learned quite some additional things, I didn’t know before. And I talked to some of my colleagues just now and they also really liked your workshop. Thanks also for providing the pdf, I will send them to the participants! I really enjoyed your presentation and I can see how dedicated you are about western blots, it was really great to listen to you!" – Max Planck Institute of Molecular Plant Physiology in Potsdam-Golm, Germany. Read more feedback here 

Read more 2024-04-25

Detect and quantify Rubisco using antibodies with 20 years of publication record

Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous, from purple bacteria to flowering plants, and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.

There are the following advantages of using Agrisera antibodies to Rubisco:
  • Working in very high dilutions, 1: 10 000 1h/RT incubation
  • Broad species reactivity, including higher plants, mosses, algae, cyanobacteria and diatoms. One and the same antibody will detect and quantify Rubisco in thousands of species
  • Suitable for Western blot, immunolocalization and tissue printing
  • Possibility of quantification in quantitative Western blot and ELISA
  • Cited in hundreds of publications, over the last 20 years

In the recent publication by Nuamzanei et al. (2024). "Impact of polyvinyl chloride (PVC) microplastic on growth, photosynthesis and nutrient uptake of Solanum lycopersicum L. (Tomato)", was investigated, using anti-RbcL antibodies from Agrisera. 

Explore and pick up suitable antibodies or kits for the detection of the most abundant protein on Earth, Rubisco.
  Rubisco from I

For images of Rubisco, and schemes in which this protein is involved, check out our free images for download here

Read more 2024-04-19

Which secondary antibody to choose for Western blot for protein detection in plant and algal samples?

A matching secondary antibody, that can be used at high dilutions, will contribute to good Western blot results, with less risk for background signal. Beyond this, it can be used for many experiments, and is therefore very cost-efficient. The differences between secondary antibodies from various suppliers are discussed here.

Agrisera's primary antibodies are produced in chicken, goat, mouse, rabbit and rat. The following high-titer secondary antibodies are appropriate matches: 
At the bottom of each primary antibody product page, you will find a recommended secondary antibody to use with it. 

*This antibody is in the group of the most published antibodies in the world, based on publication record analyses performed by CiteAB.


  Agrisera secondary antibody

If you need advice on the choice of the most suitable secondary antibody for your experiments, please ask us, either through the chat on Agrisera website, or by email.

Read more 2024-04-17

Study Photosystem I with Agrisera comprehensive antibody set

Over the last 20 years, Agrisera has developed antibodies to study Photosystem I (PSI). This protein complex plays a central role in the conversion of light energy into chemical energy, in the form of ATP and NADPH. This energy is then used to power the biochemical reactions of photosynthesis, and ultimately produce carbohydrates. Agrisera antibodies specifically targeting PSI components can help accurately identify and quantify these proteins in plant, algal and diatom samples. This enables comparative studies, and facilitates the understanding of photosynthetic processes across a wide taxonomic range.

Agrisera PSI antibodies can be applied in:

Western blotting to detect the presence of PSI subunits in plant, algae and diatom samples, which will help in understanding the expression levels of PSI proteins under different experimental conditions.

• Immunofluorescence and Immunohistochemistry to visualize the spatial distribution of PSI within plant and algal cells to study the subcellular localization of PSI and its dynamics under various physiological conditions.

• Co-immunoprecipitation (Co-IP) to identify protein-protein interactions involving PSI subunits or associated proteins. to determine the molecular interactions and protein complexes involved in PSI function and regulation.

Agrisera PSI antibodies will help determine the impact of genetic mutations on PSI function and assembly.

Browse the full collection
    Free image of photosystem I

Agrisera PSI antibodies serve as valuable tools for investigating various aspects of PSI structure, function, and regulation in photosynthetic organisms. You can rely on the strong publication record for these antibodies.

Choose a set of PSI antibodies for your studies: 

PsaB | PsaD | PsaC | PsaEPsaA | PsaK | PsaN | PsaL | PsaF | PsaH | PsaG | PsaO

Read more 2024-04-14

10% discount in return for feedback

Agrisera offers a 10% discount on any product from our catalog, in return for a short note left on the specific product page. 

Example of product reviews:

Product reviews


To leave a review:
  • Go to the product page for an Agrisera antibody or reagent you have previously used
  • Find the tab "Reviews"
  • Click on "Leave a review"
  • Write your short comment, including details like: type of extract, technique, dilution and overall result obtained with this product
  • Save your comment

Once the review is submitted, contact us at [email protected] and you will be granted a 10% discount on your next order. 

Thank you!


Read more 2024-04-12

Agrisera supports Eastern Regional Photosynthesis Conference 2024

Agrisera supports the Eastern Regional Photosynthesis Conference 2024, held between 12th and 14th of April, at The Marine Biological Laboratory (MBL) in Woods Hole, MA, USA.

The conference program will includes the following topics:

• Mechanism of Light Harvesting and Regulation
• Light Driven Reactions
• Carbon Fixation and Concentration
• Evolution and Applications of Photosynthesis

Agrisera Educational Posters
, focused on photosynthesis, will be available as hard copies for the meeting participants. Agrisera will also conduct an online quiz, with the first prize being the book Photosynthesis: Solar Energy for Life, by Dr. Dmitry Shevela, Prof. Lars Olof Björn, Prof. Govindjee.


 
 
   
Free image of PSII
 
The above image, and many other images and schemes by Scigrafik and Agrisera, can be downloaded for free here.

 

Read more 2024-04-12

Most published photosynthetic antibodies, 20 % off until end of June 2024

Agrisera's antibody collection to proteins involved in photosynthesis has been developed over the last 20 years, in close collaboration with researches in the field, from all around the world.

The collection contains antibodies targeting proteins from the following groups: 

ATPsynthase | Carotenoid metabolism | Chaperones/Transporters | Chloroplast biogenesis/architecture | Chlorophyll | Green Cut | LHC | Light acclimation | Kinases | PSI (Photosystem I) | PSII (Photosystem II) | Proteases | Protein standards-quantitation | Rubisco/Carbon metabolism | Sucrose metabolism

Each year, hundreds of publications are published, using these antibodies, which makes them the most used photosynthetic antibodies on the market. Agrisera antibodies place 31st of over 250 catalog antibody suppliers, based on publication record, not limited to plant science.


 
 
   
Photosynthesis leaf

For 20% discount for antibodies to proteins involved in photosynthesis, use promotional code Photo20. Valid until end of June 2024.

Needed antibody not found in our collection? Contact us!

Read more 2024-04-05

Versatile ECL reagent set, which can save your day!

Question: If something goes wrong with the development of a Western blot with ECL, or if I have too high background or too low signal, can I redo the reaction on the same blot?

Answer: Yes! Within the same day. 
Simply wash the membrane with the appropriate washing buffer, used in your protocol, 1-2 times for 5-10 minutes, and apply another one of the ECL reagents from the Agrisera ECL reagent set. If there is too much background, and a very strong band for the target protein, apply Agrisera ECL Bright. If a target protein band is not detected, apply Agrisera ECL SuperBright

If you want to learn more about how to improve your Western blot, click here.
 
 
   Agrisera's versatile ECL set of 2 reagents with different sensitivity

Blot A shows an abundant photosynthetic protein, PsbA, visualized with too sensitive of a reagent, which was washed away, and the membrane was subsequently developed with less sensitive Agrisera ECL Bright (Blot B). 

Read more 2024-03-28

Take advantage of Agrisera antibodies to proteins involved in response to plant pathogen attack

As plants cannot move and change their habitat quickly as animals can, they have instead developed other amazing strategies to cope with various pathogen attacks. Listed below are some examples of plant pathogens.

Fungal diseases: These pathogens typically thrive in warm, moist conditions and can infect various parts of the plant, such as leaves, stems, fruits, and roots.
Bacterial diseases: Bacterial pathogens infect plants through wounds or natural openings, causing diseases like bacterial wilt, leaf spot, and soft rot, and can spread rapidly through the whole plant.
Viral diseases: Viruses can infect plants through vectors such as insects, nematodes, and even human activities, like grafting. Viral diseases can significantly reduce crop yields and quality.
Nematode damage: Nematodes are microscopic roundworms that feed on plant roots, leading to reduced nutrient uptake and stunted growth. Root-knot nematodes, for example, cause the formation of galls on roots, interfering with water and nutrient absorption.

All antibodies to proteins involved in environmental stress response are offered at a 20% discount until the end of March. Use discount code Stress20.
 
 
 Agrisera Antibodies to proteins involved in response to pathogen attack

Read more 2024-03-23

Agrisera supports Jan Andersson Award of the Australian Society of Plant Scientists

Agrisera is sponsoring this year's Jan Anderson Award, granted by the Australian Society of Plant Scientists

Joan (Jan) Mary Anderson was an adjunct professor at the Australian National University. She received an honorary doctorate at Umeå University (Sweden) in 1998.  She was an internationally acclaimed plant biologist, renowned for her discoveries on the organization of the intricate membrane structures that carry out solar energy conversion in cells of green plants. Her work was the first to show that the photosynthetic mechanisms consists of two fundamental components; Photosystem I and Photosystem II. Her research career spanned over 55 years, mostly at the Division of Plant Industry CSIRO Canberra (1961-97), and later at the Research School of Biological Sciences at the Australian National University.

Jan was a passionate and creative female scientist who was well ahead of her time. She was an inspiration to young researchers, for whom her enthusiasm and curiosity were infectious. Professor Jan Anderson died on 28 August 2015 after a short illness. In recognition of her life and achievements in photosynthesis research as a pioneering female scientist, this award will acknowledge talented female plant science researchers. 


 
 
   Australian Society of Plant Scientists

Full description of the award

Read more 2024-03-22

Agrisera supported the 37th Molecular Biology of Plants meeting in Germany

The 37th Molecular Biology of Plants conference, organized by the Section Plant Physiology and Molecular Biology of the Germany Society for Plant Sciences, took place the 4th-7th of March, North Rhine-Westphalia, Germany. The organizers were Prof. Dr. Christopher Grefen (Bochum), Prof. Dr. Ute Höcker (Cologne), and Prof. Dr. Andreas Meyer (Bonn).

Agrisera supported the meeting with The Best Poster Prize, awarded to Dr. Marc Somssich from the Max Planck Institute for Plant Breeding Research in Cologne. The winning poster title was "Spatially resolved immune responses of Arabidopsis thaliana to colonization by Fusarium oxysporum".


Participants could also enjoy hard copies of Agrisera's Educational Posters, covering topics such as photosynthesis and epigenetics, and developed with scientists in these fields.

 
 
  Winner of Agrisera Best Poster Prize 2024

Left to right: Prof. Ute Hoecker and Dr. Marc Somssich. Courtesy of Dr. José Ugalde from the University of Bonn. 

Read more 2024-03-17

Wide species range of blocking serum for ELISA and immunolocalization

For most immunoassays, like immunolocalization, ELISA or Western blot, blocking is a crucial step. This procedure blocks spaces that are not occupied by immobilized proteins, and thus prevents non-specific binding of the antibodies used in the assay. If blocking is not performed, false signals or high background will occur.

If normal serum is used as a blocker, it is recommended to use serum derived from the same host species as the detection antibody, conjugated with a reporter enzyme, like HRP (Horseradish peroxidase) and ALP (Alkaline phosphatase), or a fluorochrome, like DyLight®, FITC or others. 

Example: 
Primary antibody: Rabbit anti-target protein

Blocking serum: Normal Chicken Serum (usually used at 5% v/v in PBS with Tween®20 detergent)

Secondary antibody: Chicken anti-Rabbit IgG-HRP conjugated

Note: Rabbit serum cannot be used for blocking in such a combination.
 
  Agrisera blocking serum
Agrisera blocking serum from various species, is provided in three purity grades: non-purified, purified on Protein G and ultrapure, available as in research and bulk quantities.

Not certain which blocker to use? Ask Agrisera.

Read more 2024-03-15
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