Light-induced structural changes in thylakoid membranes, revisited in the recent publication

In the recently published article, Wójtowicz et al. (2025). Shrink or expand? Just relax! Bidirectional grana structural dynamics as early light-induced regulator of photosynthesis, an international group of researchers challenged the traditional binary model of light-induced thylakoid dynamics. They discovered and described three-phase response, using high-resolution structural approaches like: transmission electron microscopy, confocal microscopy with 3D modeling, and small-angle neutron scattering as well as spectroscopic and electrophoretic analyses of the photosynthetic apparatus in two model species: Arabidopsis thaliana and Ficus elastica.

Selected antibodies to proteins of Photosystem I (PSI) and Photosystem II (PSII) antennae (Lhca1, Lhcb2, Lhcb4, and Lhcb5) and core proteins (D1 and PsaB), phosphorylated variants of D1 and Lhcb2 proteins and Atpb subunit of ATP synthase were used in this study. 
 
Agrisera antibodies used in this work were: 
Lhcb1-P, Lhcb2, Lhcb2-P, Lhcb4, Lhcb5, Lhca1, PsbA, PsbA-P, AtpBeta, PsaB, acetylated lysine