Agrisera blog

Agrisera Best Poster Prize awarded during XVth Meeting of Plant Molecular Biology in Spain

Our warmest congratulations to Patricia Ballester Fuentes from the lab of Dr. Cristina Ferrándiz, Instituto de Biología Molecular y Celular de Plantas (IBMCP, UPV-CSIC), in Spain, for receiving the Agrisera Award for the best poster during XV Meeting of Plant Molecular Biology, in November 2020.

The poster title was: "The NGATHA factors interact with members of the bHLH transcription factor family to direct the development of apical tissues in the Arabidopsis thaliana gynoecium."

Awaiting your free antibody choice!

Agrisera Antibody Collection to Arabidopsis thaliana can be found here.

Good luck in your research!
 Agrisera Award at XVth Meeting of Plant Molecular Biology 2020.jpg
Read more 2021-01-28

284 worldwide events in The Global Plant Events Calendar listed in 2020

We are celebrating the 2nd-year anniversary of
The Global Plant Events Calendar!

The Global calendar project was initiated on January 2nd, 2019 as a mutual effort of Plantae and Agrisera to provide the plant community with a comprehensive list of worldwide events.

284 in-person and online events like webinars, workshops, symposia and conferences, many with free registration, were registered in the calendar in 2020. Due to the pandemic, many events were cancelled, moved online or postponed, and the calendar became a valuable tool to keep track of those changes. 

Today, The Global Calendar is the most comprehensive list of worldwide events for the plant community.

If you are not familiar with the calendar this short video will show you how to use it.

Stay informed! Follow the calendar on Twitter @PlantSciEvents
 2nd Anniversary of The Global Plant Events Calendar
Read more 2021-01-25

New epitope tag antibody (BirA (mutated/TurboID) ) for protein interaction studies

Proximity labelling is a laboratory technique which makes it possible to label proteins (in this case by biotinylation), proximal to protein of interest. This allows studying protein interactome networks in different types of cells and organs, as well as different developmental stages. The technique is achieved by a gene fusion in a living cell, between the protein of interest and BioID (BirA). The TurboID variant is a biotin ligase, with substantially higher activity, which provides higher temporal resolution and broader application in vivo. The labelling time is just 10 minutes in RT, compared to the over 18 hours of labelling time required for BioID. 

Agrisera offers a new polyclonal antibody, prepared to a recombinant mutated BirA protein from E.coli, called TurboID.
anti-BirA (mutated/TurboID)

The proximity labelling technique is described in various publications, e.g.:
Zhang et al. (2020). TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks.J Vis Exp. 2020 May 17;(159).doi: 10.3791/60728.
Cho et al. (2020). Proximity labeling in mammalian cells with TurboID and split-TurboID.Nat Protoc. 2020 Dec;15(12):3971-3999. doi: 10.1038/s41596-020-0399-0.
Mair et al. (2019). Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864.
 Western blot TurboID
For experimental details, click here.
Read more 2021-01-24

25 % off on anti-TAG antibodies

Tag antibody promotion

Antibodies to tags are among the most published antibodies in the world, according to analysis of publications peformed every year by CiteAb. Their application is broad, from recombinant protein purification and detection, to visualization of a target protein in transgenic organism, when antibody to endogenous protein is not available or there are difficulties to produce it due to the target protein levels being beyond the detection level. 
Tags can be few amino acids long, like His, FLAG and HA, or be made to whole proteins, like GST or GFP. 

The Agrisera anti-Tag antibody promotion covers the following antibodies:

AS09 580 | aadA1 | Aminoglycoside adenyltransferase (chloroplast transformation marker)
AS20 4485 | BFP, GFP, YFP | Fluorescence Protein, clone 3A6
AS20 4454 | c-myc (monoclonal, Clone 9E10)
AS09 601 | c-myc (polyclonal)
AS20 4379 | DYKDDDDK (binds to Sigma FLAG®) (chicken antibody, polyclonal)
AS15 2871 | DYKDDDDK (binds to Sigma FLAG®, clone FG4R)
AS15 2987 | GFP | Green Fluorescence Protein (affinity purified)
AS15 2861 | GST-tag (mouse monoclonal, clone GST.B6)
AS17 4147 | GST-tag (rabbit antibody, polyclonal)
AS16 3689 | GUS | Beta-glucuronidase
AS20 4463 | HA (chicken polyclonal)
AS20 4457 | His-tag | 6xHis (monoclonal, Clone 2C5.1)
AS19 4255 | His-tag | 6xHis (polyclonal)
AS16 3691A | LUC | Luciferase (firefly) (affinity purified antibodies)

Use discount code: Tag2021
Valid January-December 2021

Read more 2021-01-21

Agrisera Antibody Quiz winner at the Annual Western Photosynthesis meeting 2021

Congratulations to Daiki Nishiori, who participated in Agrisera Antibody Quiz during the 30th Western Photosynthesis Conference, sponsored by Agrisera, held online over Saturday, January 2nd and Saturday, January 9th, 2021. 

Daiki Nishiori is a 3rd year Chemistry PhD student working in Prof. Gary F. Moore's lab at School of Molecular Sciences of Arizona State University. He is doing research on the synthesis and characterization of porphyrin compounds that can catalyze hydrogen evolution reactions and oxygen reduction reactions. He also develops hybrid artificial photosynthetic assemblies composed of molecular catalysts and light-harvesting semiconductors.

Agrisera Antibody Quiz consisted of 11 questions and the winner of the quiz:
Daiki Nishiori will receive a hard copy of the book:

Photosynthesis Solar Energy for Life by Dr. Dmitry Shevela, Prof. Lars Olof Björn, Prof. Govindjee Hardcover: 204 pages Publisher: World Scientific Publishing Company (7 Jan. 2019)

Best biophysics book of all time Best biophysics book of all times

The book is available in Agrisera webshop.


Read more 2021-01-13
 Agrisera blog


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