Agrisera News

Interview with Dr. Heni Hitaj

Dr. Heni Hitaj

Heni Hitaj

Heni Hitaj is a PhD student and Researcher in Prof. Margarete Baier’s lab (Baier Group) at the Freie University in Berlin, Germany. Hitaji's research explores how plants counteract photooxidative stress induced by reactive oxygen species (ROS) in chloroplasts, with the aim to enhance the understanding of plant stress responses and resilience to environmental changes.



- Please tell us about yourself and your research/institution.

My name is Heni Hitaj and I am doing my PhD at the Freie University in Berlin, specializing in antioxidative defense systems in plants. My research focuses on unraveling the mechanisms by which plants mitigate photooxidative stress caused by reactive oxygen species (ROS) in chloroplasts. Understanding these processes is crucial for optimizing plant stress responses and improving resilience to environmental fluctuations. We aim to provide a comprehensive understanding of thylakoid membrane dynamics and the regulatory role of tAPX, shedding light on how plants fine-tune their antioxidant defenses against photooxidative damage.



- What motivated you to get into plant science?

Being an up-and-comer from the new generation of plant scientists, I am acutely aware of the urgent challenges our crops face today. Climate change, extreme weather, and environmental stressors are no longer distant threats—they are happening right now, impacting global food security and pushing plants to their limits. Seeing these issues unfold, I knew I wanted to make a difference and be part of the solution. I’ve always been fascinated by how plants, despite being rooted in place, have evolved sophisticated defense mechanisms to survive harsh conditions. Unlike animals, they can’t escape threats—they must adapt. This resilience, especially in response to oxidative stress and reactive oxygen species (ROS), inspired me to focus my research on antioxidative defense systems. If we can better understand how plants protect themselves at the cellular level, we can engineer stronger, more resilient crops capable of withstanding the unpredictable climate.



- How have you used (Agrisera) antibodies in your research?

I use Agrisera antibodies routinely in my current research projects to characterize the dynamics of new protein candidates, particularly in understanding their abundance, localization, and interactions. For example, I apply these antibodies in Co-Immunoprecipitation (Co-IP) experiments to identify potential interaction partners of key proteins involved in oxidative stress regulation. Across all the different research groups I’ve been a part of, Agrisera has become a household name for antibody selection. Whenever I’m designing an experiment, I always check if Agrisera has a relevant antibody before considering alternatives. Their products have become my go-to choice for high-quality, dependable reagents.


- Any further comments?

It is always a pleasure meeting Joanna at conferences. I have always been a fan of Agrisera, and having the opportunity to speak with her gave me valuable insight into how the company operates and the passion that goes into developing high-quality antibodies. It’s inspiring to see the dedication behind the products that so many researchers, including myself, rely on for our work.



Links

• Baier Group, Freie Universität Berlin
• Agrisera antibodies to environmental stress




Read more 2025-04-30

Interview with Prof. Eiji Nambara

Prof. Eiji Nambara

Eiji Nambara

Dr. Eiji Nambara is a professor at the University of Toronto, Canada. He completed his PhD in Virology at Nagoya University, Japan, and was the recipient of the Japanese Society for Plant Physiologists Young Investigator Award in 2006, as well as the 2010 Research Award, granted by the Japanese Society for Chemical Regulation. Dr. Nambara studies how hormone levels are regulated, focusing on elucidating the molecular mechanisms that control abscisic acid metabolism and transport.


- Please tell us about yourself and your research/institution.

I am a professor in the department of Cell and Systems Biology, at the University of Toronto. My lab has studied the role of ABA catabolism in seed maturation, dormancy, and germination, including the first identification of CYP707As as ABA 8'-hydroxylase genes. We also found that environmental changes, such as nitrate and humidity, alter the expression of ABA catabolism genes as primary responses. My interest in studying ABA began with my MSc supervisor, Prof. Peter McCourt. The continuation of this topic was influenced by Dr. Yuji Kamiya, a group director at the RIKEN Plant Science Center, where I had my first lab. I believe ABA is fascinating because it acts as a mobile signal to manage water relations in plants. A benefit of studying plant hormones is the fact that multidisciplinary skills, such as those in chemistry and biology, are required.


- What motivated you to get into plant science?

I studied organic chemistry as an undergraduate student at Nagoya University, Japan. The lab I was part of focused on organic reactions that mimic biological systems. I was fascinated by the elegant organization of metabolic systems in organisms. The diverse metabolites produced by plants attracted me to move into plant biology.


- How have you used (Agrisera) antibodies in your research?

Yes, my PhD student Ben has used Agrisera’s antibodies against AtABA2 that encodes xanthoxin dehydrogenase in ABA biosynthesis.


- Any further comments?

My first interaction with Agrisera was in 2016 when they kindly supported the International Plant Growth Substances Association (IPGSA) meeting in Toronto.



Links

• Prof. Eiji Nambara, Univeristy of Toronto
• Agrisera antibodies to plant hormones




Read more 2025-04-16

Agrisera anti-Actin antibody applied in a new technique termed Expansion microscopy in plant PrOtoplast SystEms (ExPOSE)

While the technique of expansion microscopy (ExM) is commonly used in animal cells and tissues, plant-specific protocols are very few. 

Agrisera's collaboration partner, Dr. Kirk Czymmek (Danforth Plant Science Center, USA) and his colleagues from several other American institutions, recently published a method that allows nonoscale imaging by physical expansion of fixed plant protoplasts in a swellable hydrogel. This method, called ExPOSE, leads to enhancement of the resolution by several folds, and shows that, combined with other imagining techniques, like immunofluorescence and in situ hybridization chain reaction, it can lead to visualization of proteins and mRNAs with enhanced spatial resolution.

ExPOSE opens new avenues for discoveries in studies of plant cells on the molecular level, as it circumvents the resolution limitations of traditional microscopy methods, and allows the revealing of finer details of cellular structures.

Read the recently published article "ExPOSE: a comprehensive toolkit to perform expansion microscopy in plant protoplast systems".

Cited Agrisera antibodies:

Anti-GDC-H | H protein of glycine decarboxylase complex (GDC) (AS05 074)
Anti-ACT | Actin (polyclonal) (AS13 2640)
Donkey anti-Rabbit IgG (H&L), DyLight® 594 conjugated, pre-adsorbed (AS12 2076)
 Anti-actin antibody applied in expansion microscopy

 Agrisera polyclonal antibody to plant actin (AS13 2640) was applied to visualize actin filaments using expansion microscopy (ExM) on Arabidopsis thaliana protoplasts. Details of the method are described here


 

Read more 2025-04-11

Interview with Prof. Rossana Henriques

Prof. Rossana Henriques

Rossana Henriques


Dr. Rossana Henriques is the Paddy O'Keeffe Senior Lecturer (Associate Professor) in Plant Genetics at the School of Biological, Earth and Environmental Sciences at University College Cork (UCC), a role she has held since 2018. Prior to this, she was a junior Principal Investigator at the Centre for Research in Agricultural Genomics (CRAG) in Barcelona, and a Research Associate in Professor Nam-Hai Chua's lab at The Rockefeller University in New York. She was a postdoctoral fellow at Royal Holloway College, University of London, and completed her PhD through a collaboration between the University of Lisbon and the Max-Planck Institute in Cologne.



- Please tell us about yourself and your research/institution.

I'm interested in the regulation of plant growth, and how that connects to seasonal conditions. I.e. how plants measure time and use that information to control their growth patterns. More specifically, I study how plants use day length and temperature cues to modulates their growth and development responses. At University College Cork, where I've been for the last 7 years, we have continued the research I was previously conducting at the Centre for Research in Agricultural Genomics, examining how the TOR pathway regulates growth responses. Initially, we used Arabidopsis, but we are expanding the studies to include forage grasses and legumes.

We are studying how plants grow under short and long day conditions, and how that correlates with temperature. Considering that climate change is increasing the average temperature on Earth, leading to extreme events, like periods of drought or flooding, we have also done research assessing how a higher temperature, and some abiotic stress factors, impact growth responses. Beyond that, we are examining what happens with components of the TOR pathway when the environmental conditions are not optimal. Besides TOR, we have also done some work with long non-coding RNAs, which connects to both abiotic stress responses and developmental questions, like flowering time regulation.



- What motivated you to get into plant science?

My degree is in biology, but I knew that I wasn't going to be a zoologist or ecologist. I've always been more interested in molecular biology and biochemistry, but from a plant perspective. Many signaling pathways are well-studied and understood in animal research, but not in plant science. I've always been keen on trying to see how we could answer questions that were already well-established in animal research, using plants. So, developing new methodologies and setting up new strategies, to address e.g. how plant proteins accumulate. I find it very interesting how plants do things, and what the molecular mechanisms that underlie their responses are. From the plant's perception of what is going on, and how that translates into specific decisions at a cellular level, to how this impacts organs, and ultimately the organism itself. Plants are very plastic, and have many particular abilities. Any plant cell could be a stem cell, given the right conditions. This is something that has recently gained traction in animal research, while in plant research, we've been doing tissue culture, and making leaves into embryos since the '60-70s. Something I've always liked with plants, is the ability to work with different tissues and address a lot of different questions. There is also the fact that without plants, there really is no life on this planet. A lot of people don't realize how vital plants are to food security and our life.



- How have you used (Agrisera) antibodies in your research?

I've been collaborating with Agrisera for several years, and I think it's brilliant that there is a company that really provides researchers in plant science with appropriate tools. For a long time, I struggled with working with antibodies that were published in research for animals. All the Western blots, immunoprecipitations and data from animal publications looked brilliant, but when you tried to replicate it with plant material, many of those things didn't work. I was really happy when we started our collaboration and interaction with Agrisera. There was finally a company that really put their resources and efforts into working for the plant science community.

I am very grateful for Agrisera's strong collaboration with scientists, and in our specific case, the development of several antibodies for the TOR pathway. We wouldn't have been able to do the work we have done without your antibodies. It allowed us to do a lot of research in characterizing the TOR pathway. I think this is what makes Agrisera a special company, for me as a plant biologist.

I would also like to highlight the fact Agrisera is always interested in new antibody suggestions from the plant science community, and the fact that some of us can try the antibodies, validate them, and determine the best conditions for detection. It's very useful that you have the data from researchers, with whom you've collaborated, presented on your website. You show the actual Western blots, and the conditions for them. I really appreciate that this allows others to follow all the instructions on how to use your antibodies.

To me, another important aspect is that cross-reactivity is specified for your antibodies. Arabidopsis has been very good to us, in terms of a model species, allowing us to ask a lot of questions, and develop common tools. But with broad antibody species reactivity, we can allow ourselves to ask other questions, and move on to other species, connected to food security.




Links

Prof. Rossana Henriques, University College Cork
Agrisera antibodies to proteins involved in Plant Signal Transduction
Agrisera antibodies to proteins involved in environmental stress response



Read more 2025-04-02

New antibody: Anti-FTIP3/FTIP4 | FT Interacting Protein 3/4

New Agrisera antibodies to FTIP3/FTIP4 | FT Interacting Protein 3/4!

Background
FT Interacting Proteins 3 and 4 (FTIP3 and FTIP4) are crucial components in the regulation of plant development, particularly in the maintenance and function of the shoot apical meristem (SAM) in Arabidopsis thaliana. These proteins belong to the Multiple C2 domain and Transmembrane Region Proteins (MCTP) family.

Ongoing research aims to uncover the specific molecular pathways and interactions involving FTIP3 and FTIP4, as well as their potential applications in agriculture, such as crop improvement and adaptation strategies. Understanding the functions of FTIP3 and FTIP4 enhances our comprehension of plant developmental biology and offers insights into the complex regulatory networks that govern plant growth and adaptation.


Product: Anti-FTIP3/FTIP4 | FT Interacting Protein 3/4

Product number: AS23 5001

Confirmed reacitivity: Arabidopsis thaliana

Predicted reactivity: Arachis hypogaea, Capsicum annuum, Brassica napus, Cannabis sativa, Cucumis sativus, Glycine max, Gossypium sp., Malus domestica, Manihot esculenta, Medicago truncatula, Nicotiana tabacum, Phaseolus vulgaris, Pisum sativum, Ricinis communis, Solanum lycopersicum, Solanum tuberosum, Spinacia oleracea, Vitis vinifera (more info on predicted reactivity)

Tested applications: Western blot (Western blot resources)

Matching secondary antibody: Goat anti-Rabbit IgG (H&L), HRP conjugated (AS09 602)

 Application example
Anti-FTIP3/FTIP4 | FT Interacting Protein 3/4
Samples:
mctp3 mctp4 – Arabidopsis thaliana double knockout mutant
mctp3Arabidopsis thaliana single knockout mutant
mctp4Arabidopsis thaliana single knockout mutant
Wild type – Arabidopsis thaliana wilde type

Read more 2025-04-01
 Agrisera News

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