ACT | Actin (polyclonal)
AS13 2640 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Agostis stoloniferacv. ‘Penncross’,Arabidopsis thaliana, Brassica sp., Cannabis sativa L., Cucumis sativus, Cynara cardunculus, Glycine max, Hordeum vulgare, Nicotiana tabacum, Phoenix dactylifera, Picrorhiza kurroa, Setaria italica, Solanum tuberosum, Triticum aestivum, Zea mays
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ca. 100 amino acids of recombinant actin conserved more than 80 % in Arabidopsis thaliana: actin-1 P0CJ46 AT2G37620, actin-2 Q96292 AT3G18780, actin-3 P0CJ47 AT3G53750, actin-4 P53494 AT5G59370, actin-5 Q8RYC2 At2g42100, actin-7 P53492 At5g09810, actin-8 Q96293 AT1G49240 , actin-11 P53496 , AT3G12110 ,actin-12 P53497 AT3G46520
Agropyron cristatum, Beta vulgaris, Betula luminifera, Brassica napus, Brassica rapa subsp. pekinensis, Cannabis sativa L. , Capsella rubella,Castanea sativa, Chorispora bungeana, Cyanidioschyzon merolae strain 10D, Glycine soja, Halogeton glomeratus, Manihot esculenta, Medicago truncatula, Malus domestica, Oryza sativa, Pisum sativun, Populus sp., Solanum lycopersicum, Solanum tuberosum, Phaseolus vulgaris, Phaeodactylum tricornutum, Picea abies, Picea sitchensis, Prunus avium, Ricinus communis, Rubus plicatus, Theobroma cacao,Vicia faba
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Chlamydomonas reinhardtii (too high background for this species)
15 µg of total protein extracted with PEB (AS08 300) from leaf tissue of (1) Arabidopsis thaliana, (2) Hordeum vulgare, (3) Zea mays were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-actin (AS13 2640, 1:2500, 1h) and secondary anti-rabbit (1:10 000, 1 h) antibody (HRP conjugated, recommended secondary antibody AS09 602) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent using a Fuji LAS-3000 CCD (300s, standard sensitivity). Exposure time was 2 min.
Actin cytoskeleton in 5 days old Arabidopsis thaliana seedlings. Actin signal shown in green, PIN1 in red and DAPI in blue. The material has been fixed in 2 % formaldehyde for 45 minutes. Tissue cleaning has been performed before immunolocalization. Rabbit anti-actin primary antibody was diluted in 1:250 and anti-rabbit Alexa 488 and Alexa 555 were both diluted in 1: 500 (Invitrogen). Scale bar - 20 µm.
Courtesy: Dr. Taras Pasternak, Freiburg University, Germany
Courtesy of Iauhenia Isayenka, University of Sherbrooke, Canada
This product can be sold containing ProClin if requested.
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
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