Chromogenic Detection

Colorimetric detection employs chromogenic substrates, which can be either alcohol insoluble - appropriate for Western blot and IHC, or alcohol soluble dyes, which are suitable for ELISA. The blot is incubated with a chromogenic substrate to yield a colored precipitation. Signal development is arrested by washing off the substrate. Resulting precipitate is visualized directly on a membrane as a band or a dot.

Chromogenic substrates are available for various enzymes: HRP (horse radish peroxidase), ALP (Alkaline phosphatase) , GOD (Glucose oxidase) and β-Gal (β galactosidase).
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From 41 €
AS18 WestBlock | Non Mammalian Protein Membrane Blocker

Agrisera Non Mammalian Protein Membrane Blocker is a Western Blot friendly formulation for optimal blocking effect.

  • Ready to use, no mammalian protein included
  • Stable for a minimum of 2 years
  • Contains antimicrobial agents (Bromidox, 0.08%) to prevent bacterial growth
From 63 €
AS19 BCIP-NBT | Ready to use, single component BCIP/NBT reagent
From 84 €
AS19 BCIP-NBT-PLUS | Ready to use, single component BCIP/NBT reagent