PSII (Photosystem II)
PSI/PSII ratio can be estimated once a quantitative immunoblot for PsaC (AS10 939) and for either PsbA (AS05 084) or PsbD (AS06 146), is performed in 3-4 replicates. Ratio calculation is done on a sample by sample basis. These ratios are based on the subunits present, whether they are in the assembled photosystem or not. Under some circumstances (eg. high light), there are biological reasons that the subunit numbers do not necessarily match the functional photosystems.
Literature
Brown et al. (2008). Flux capacities and acclimation costs in Trichodesmium from the Gulf of Mexico. Marine Biology 154 (3): 413-422.
Levitan et al. (2010). Regulation of nitrogen metabolism in the marine diazotroph Trichodesmium IMS101 under varying temperatures and atmospheric CO2 concentrations. Environmental Microbiology 12(7): 1899-1912.
Thamatrakoln et al. (2013). A ‘Death-Specific Protein’ in a marine diatom regulates photosynthetic responses to iron and light availability. Proceedings of the National Academy of Science 110(50): 20123-20128 Li G,
Li et al. (2014). The Nitrogen Costs of Photosynthesis in a Diatom under Current and Future pCO2. New Phytologist, 205:533-543.
AS08 300 | Extraction buffer for quantitative isolation of total soluble/membrane protein from plant tissue and algal/bacterial cells, optimized for subsequent western blot detection in denatured conditions
AS22 4733 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii
Hardcover: 204 pages Publisher: World Scientific Publishing Company (7 Jan. 2019)
Language: English
ISBN-10: 9813223103 ISBN-13: 978-9813223103
Product Number: AS19 4290Book ; Product Dimensions: 15.2 x 1.9 x 22.9 cm
AS06 111 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Cyanobacteria
Back in stock in December 2023.
The set is suitable for Western blot using samples from a wide range of species making this product suitable for teaching labs or mutant screening.
Benefits of using this antibody set
This discounted antibody set contains the following high titer, well published antibodies and is suitable for Western blot using samples from a wide range of species making. This product suitable for teaching labs or mutant screening.
Benefits of using this antibody set
This set includes three discounted, well published, compartment marker antibodies of your choice. Benefits of using this antibody set
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AS05 084B | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms
compartment marker of thylakoid membrane
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AS10 704 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, C.reinhardii, H.vulgare, M.truncatula, N. oceanica, N. oleoabundans UTEX 1185 (chlorophyta), P.patens, P.sativum, S.vulgaris, S.alba, S.oleracea, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, Triticum sp.
AS11 1786 | Clonality: Polyclonal | Host: Rabbit | Reactivity: higher plants, alage, cyanobacteria
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AS09 146S | Recombinant protein standard for quantitation and positive control
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AS06 112 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, H. vulgare, N.tabacum, O. sativa, S. oleracea | cellular [compartment marker] of thylakoid membrane
AS19 4330 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Synechocystis sp. PCC6803
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AS10 692 | Clonality: Polyclonal | Host: Rabbit | Reactivity: cyanobacteria
AS06 158 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, H.vulgare
AS14 2786 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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