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PsbE | Cytochrome b559 subunit alpha (Algal)

AS19 4330   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Synechocystis sp. PCC6803

PsbE | Cytochrome b559 subunit alpha (Algal) in the group Antibodies for Plant/Algal  / Chlamydomonas reinhardtii at Agrisera AB (Antibodies for research) (AS19 4330)

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Product Information

Immunogen KLH-conjugated peptide derived from algal PsbE sequences, including Chlamydomonas reinhardtii PsbE UniProt: P48268
Host

Rabbit

Clonality Polyclonal
Purity Affinity purified serum, in PBS pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 9 kDa

Reactivity

Confirmed reactivity Synechocystis PCC 6803
Predicted reactivity Bathycoccus prasinos, Capsosiphon fulvescens, Chlamydomonas sp., Gonium pectorale,Mesostigma viride, Microglena monadina, Nannochloropsis granulata,Neglectella solitaria, Ostreococcus tauri, Pandorina morum, Planctonema lauterbornii, Thermosynechococcus vulcanus, Tupiella akineta, Ulva lactuca, Volvulina compacta, Volvox africanus, Yamagishiella unicocca

Species of your interest not listed? Contact us
Not reactive in higher plants

Application examples

Application examples

Western blot using anti-PsbE antibodies (Algal)

Samples:
1 - 10 μg of Synechocystis PCC 6803 isolated thylakoid membranes
2 - 20 μg of Synechocystis PCC 6803 isolated thylakoid membranes
3 - 10 μg of PsbE deletion mutant Synechocystis PCC 6803 isolated thylakoid membranes

10 or 20 µg/well of total protein from isolated thylakoid membranes with MES buffer (25mM MES/NaOH, pH 6.5, 10 mM MgCl2, 10 mM CaCl2, and 25% glycerol) and denatured with SDS-solubilizing buffer stock (5 % SDS in 25 mM Tris-HCl, pH 7, 1M sucrose) with working concentrations 1% SDS and 2% DTT at 23°C for 1 hour were separated on 16 - 20 % AA, 7 M urea SDS-PAGE and blotted 1h to PVDF (pore size of 40 μm), using wet transfer. Blot was blocked with 0.2 % Tween in TBS for 30 min/RT with agitation. Blot was incubated in the PsbE primary antibody at a dilution of 1: 1 000 overnight at 4°C with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 5 min in TBS-T at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera secondary antibody (AS09 602) diluted to 1:25 000 in TBS-T for 1h/RT with agitation. The blot was washed as above, incubated for 5 min in AgriseraECL Bright (AS16 ECL-N) and developed in LAS-4000 camera (Fujifim, Japan). Exposure time was 1 minutes.

Courtesy Dr. Josef Komenda, ALGATECH, Czech Republic

Additional information

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Background

Background PsbE (Cytochrome b559 subunit alpha) is tightly associated with the reaction center of photosystem II (PSII).
Alternative name: PSII reaction center subunit V

Product citations

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