PsbE | Cytochrome b559 subunit alpha (Algal)

Product no: AS19 4330

AS19 4330   | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Synechocystis sp. PCC6803

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from algal PsbE sequences, including Chlamydomonas reinhardtii PsbE UniProt: P48268
    Host:

    Rabbit

    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l, of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 9 kDa
  • Reactivity
  • Confirmed reactivity: Synechocystis PCC 6803
    Predicted reactivity: Bathycoccus prasinos, Capsosiphon fulvescens, Chlamydomonas sp., Gonium pectorale,Mesostigma viride, Microglena monadina, Nannochloropsis granulata,Neglectella solitaria, Ostreococcus tauri, Pandorina morum, Planctonema lauterbornii, Thermosynechococcus vulcanus, Tupiella akineta, Ulva lactuca, Volvulina compacta, Volvox africanus, Yamagishiella unicocca

    Species of your interest not listed? Contact us
    Not reactive in: higher plants
  • Application Examples
  • Western blot using anti-PsbE antibodies (Algal)

    Samples:
    1 - 10 μg of Synechocystis PCC 6803 isolated thylakoid membranes
    2 - 20 μg of Synechocystis PCC 6803 isolated thylakoid membranes
    3 - 10 μg of PsbE deletion mutant Synechocystis PCC 6803 isolated thylakoid membranes

    10 or 20 µg/well of total protein from isolated thylakoid membranes with MES buffer (25mM MES/NaOH, pH 6.5, 10 mM MgCl2, 10 mM CaCl2, and 25% glycerol) and denatured with SDS-solubilizing buffer stock (5 % SDS in 25 mM Tris-HCl, pH 7, 1M sucrose) with working concentrations 1% SDS and 2% DTT at 23°C for 1 hour were separated on 16 - 20 % AA, 7 M urea SDS-PAGE and blotted 1h to PVDF (pore size of 40 μm), using wet transfer. Blot was blocked with 0.2 % Tween in TBS for 30 min/RT with agitation. Blot was incubated in the PsbE primary antibody at a dilution of 1: 1 000 overnight at 4°C with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 5 min in TBS-T at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera secondary antibody (AS09 602) diluted to 1:25 000 in TBS-T for 1h/RT with agitation. The blot was washed as above, incubated for 5 min in AgriseraECL Bright (AS16 ECL-N) and developed in LAS-4000 camera (Fujifim, Japan). Exposure time was 1 minutes.

    Courtesy Dr. Josef Komenda, ALGATECH, Czech Republic

  • Background
  • Background: PsbE (Cytochrome b559 subunit alpha) is tightly associated with the reaction center of photosystem II (PSII).
    Alternative name: PSII reaction center subunit V
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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Buy 2 items of this product for 226.00 €/items
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