Best practices for Western Blot - interviews with experts
Store precious samples on a membrane instead of a freezer!
Save precious time in the lab, check antigen conservation before antibody purchase
Background signal and a sensitivity of ECL reagent
Tubulin alpha and gamma are not suitable as a loading control for metal stress
What can protein extraction buffer change in a Western blot?
Antibody validation strategies - crucial to secure antibody quality
What Western blot conditions to apply for high abundance proteins?
Same Western blot protocol for all antibodies?
Free antibody sample, is it always the best solution?
What to consider before antibody purchase?
Protein extraction, a key to success in Western blot
No signal on Western blot, what to do next?
Is sample dentaturation above 95°C, the golden standard?
Polyclonal Antibody in serum or affinity purified, what is a difference and how can it influence my results?
What are the most important factors for a successful antibody production?
Why is pre-immune serum screening of importance before antibody production starts?
Do secondary antibodies differ only in price?
Is the antibody always to blame?
TBS-T or PBS-T, does it matter which buffer is used for antibody incubations?
What is a specific antibody concentration?
FAQs about Catalog Antibodies
FAQs about Antibody Production Process
What is the safest method to store antibodies?
Is an antibody not specific or Western blot protocol is not optimized?
Common misconceptions about antibodies - II
Common misconceptions about antibodies - I
How to cite the antibodies used in scientific publications?
Save time! Use simultaneous incubation with few primary antibodies at the same time.
Which secondary antibodies to use when my protein was immunoprecipiated using an antibody?
Rapid Western blotting
How to know that a commercial antibody is going to work on a protein from my species?
Is a standarized reporting of research antibody use - a way to increase experimental reproducibility?
Agrisera protein extraction buffer and antibodies used to validate high throughput protein extraction from tough marine phytoplankton