Technical tips

Are blocking conditions nothing to pay attention to?

Read more

2026-06-18

What are the most common misconceptions about primary antibodies?

Read more

2026-05-27

What are the most common mistakes done with secondary antibodies?

Read more

2026-05-26

Simple extraction protocol for membrane proteins

Read more

2026-04-17

How to improve signal with a weak antibody?

Read more

2026-03-26

Same antibody, different outcome on samples from wide taxonomic range

Read more

2026-02-23

Can the quality of protein transfer be checked before the blot is developed with antibodies?

Read more

2026-01-16

Is wild type a wild type?

Read more

2025-12-29

Why an antibody may detect tagged protein but not endogenous one and in some cases endogenous protein but not its tagged version?

Read more

2025-10-30

Calclulated and aparent molecular weight of detected protein is different, why?

Read more

2025-10-09

How to chose right loading control for Western blot?

Read more

2025-10-06

Is an antibody going to work in a technique I am planning to use it in?

Read more

2025-09-30

Antibody reactivity to recombinant protein,does not validate antibody specificity in endogenous sample

Read more

2025-08-20

Can aggregated antibody be still used?

Read more

2025-08-15

Blot Once, Probe Twice - when such approach can be beneficial?

Read more

2025-07-31

Why should glycerol not be added, to stabilize antibodies in serum?

Read more

2025-06-27

Can methanol in transfer buffer be replaced by a less toxic alternative?

Read more

2025-05-12

Will the precipitation of SDS in the stripping buffer affect antibody stripping efficiency?

Read more

2025-04-29

How to check if protein transfer from a gel to a membrane was efficient

Read more

2025-04-15

How to detect membrane proteins on Western blot?

Read more

2025-03-27

Rinse Right: The Hidden Power of Washing in Western Blots

Read more

2025-02-11

Can one Western blot protocol fit all antibodies?

Read more

2025-02-07

How to reconstitute lyophilized antibodies?

Read more

2025-01-17

What information to look for before purchasing an antibody?

Read more

2024-12-30

Membrane activation before protein transfer: which one to use, methanol or ethanol?

Read more

2024-12-23

Comparing antibodies between each other, why does it not work?

Read more

2024-11-25

How to remove cross-reactivity to Rubisco of a primary antibody?

Read more

2024-09-24

Which is an optimal buffer for protein extraction?

Read more

2024-07-29

Weak target protein bands and strong background at a top of a gel, what can be a reason for that?

Read more

2024-06-19

Why do some proteins always show as double bands on Western blot?

Read more

2024-05-30

How to further denature proteins transferred to PVDF membrane?

Read more

2024-05-22

Which isotype is a rabbit polyclonal antibody?

Read more

2024-05-06

Why bands on the blot are not distinct from each other?

Read more

2024-05-02

What parameters should be consider when transferring HMW proteins?

Read more

2024-03-01

Why some antibodies have a broad species reactivity, while the others do not?

Read more

2024-02-20

Are directly conjugated primary antibodies always the best choice?

Read more

2023-12-28

How to obtain sharper protein bands in Western blot?

Read more

2023-12-20

Are monoclonal antibodies better, compared to polyclonal antibodies?

Read more

2023-11-30

Why, to detect some proteins, 6M urea must be included in the extraction and loading buffers?

Read more

2023-09-01

I have not used this antibody before, what conditions of Western blot do you recommend?

Read more

2023-07-27

Which subclasses of immunoglobulin G are found in rabbit serum?

Read more

2023-07-18

Antibody suddenly does not work any longer, what can be the reason?

Read more

2023-07-11

Can an antibody made to a full-length protein distinguish between 2 isoforms?

Read more

2023-05-10

What is a reason for so called "ghost bands" to appear on blots?

Read more

2023-03-30

One of the ways to remove background on a Western blot

Read more

2023-03-24

How to predict in which technique a given antibody may work?

Read more

2023-03-10

Which capture antibody to use in ELISA?

Read more

2023-03-06

Why should we measure protein concentration?

Read more

2023-03-03

Can results obtained in Western blot be representative for other techniques?

Read more

2023-01-31

Effect of a membrane wash on background signal in Western blot

Read more

2022-12-16