Technical blog

2023-09-01
Why, to detect some proteins, must 6M urea be included in the extraction and loading buffers?
2023-07-27
I have not used this antibody before, what conditions of Western blot do you recommend?
2023-07-18
Which subclasses of immunoglobulin G are found in rabbit serum?
2023-07-11
Antibody suddenly does not work any longer, what can be the reason?
2023-05-10
Can an antibody made to a full-length protein distinguish between 2 isoforms?
2023-03-30
What is a reason for so called "ghost bands" to appear on blots?
2023-03-24
One of the ways to remove background on a Western blot
2023-03-10
How to predict in which technique a given antibody may work?
2023-03-06
Which capture antibody to use in ELISA?
2023-03-03
Why should we measure protein concentration?
2023-01-31
Can results obtained in Western blot be representative for other techniques?
2022-12-16
Effect of a membrane wash on background signal in Western blot
2022-12-15
How to check predicted species reactivity of an antibody before purchase is done?
2022-11-30
Which membrane to use for protein transfer?
2022-10-31
How to visualize a target protein in a Western blot?
2022-10-03
TBS or TBS-T, how using respective buffer for primary antibody incubation may change the outcome
2022-10-03
What are the benefits of antibodies provided in lyophilized format?
2022-09-09
What conditions to apply for detection of low abundance proteins?
2022-09-01
Can primary antibodies be re-used?
2022-08-10
How antibodies are validated - interviews with experts
2022-08-01
Which secondary, fluorescent dye conjugated antibody to use with ECFP/FITC/TRIC filter set?
2022-06-20
Advice for beginners and experienced users of Western blot technique
2022-05-16
How to save time and shorten Western blot protocol?
2022-03-21
How to transfer proteins of low molecular weight?
2022-03-16
What are the benefits of using directly conjugated primary antibodies?
2022-03-11
Antigen, immunogen and epitope, what is the difference?
2022-02-21
How to get an intense signal in Western blot using a weak antibody?
2022-01-19
How much of a specific antibody can be found in serum?
2021-11-26
What does confirmed or predicted reactivity of an antibody mean?
2021-11-15
What determines primary antibody dilution?
2021-11-10
Affinity purified antibody, what does it really mean for the end user?
2021-10-31
Can knowing the antigen sequence help identify background bands in a Western blot?
2021-08-18
Best practices for Western Blot - interviews with experts
2021-06-30
Store precious samples on a membrane instead of a freezer!
2021-05-29
Save precious time in the lab, check antigen conservation before antibody purchase
2021-04-30
Background signal and a sensitivity of ECL reagent
2021-03-17
Tubulin alpha and gamma are not suitable as a loading control for metal stress
2021-03-12
What can protein extraction buffer change in a Western blot?
2021-02-28
Antibody validation strategies - crucial to secure antibody quality
2021-01-13
What Western blot conditions to apply for high abundance proteins?
2020-12-18
Same Western blot protocol for all antibodies?
2020-11-01
Free antibody sample, is it always the best solution?
2020-10-12
What to consider before antibody purchase?
2020-09-30
Protein extraction, a key to success in Western blot
2020-08-31
No signal on Western blot, what to do next?
2020-07-27
Is sample dentaturation above 95°C, the golden standard?
2020-06-30
Polyclonal Antibody in serum or affinity purified, what is a difference and how can it influence my results?
2020-06-16
What are the most important factors for a successful antibody production?
2020-06-08
Why is pre-immune serum screening of importance before antibody production starts?
2020-06-02
Do secondary antibodies differ only in price?

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