Why, to detect some proteins, must 6M urea be included in the extraction and loading buffers?
I have not used this antibody before, what conditions of Western blot do you recommend?
Which subclasses of immunoglobulin G are found in rabbit serum?
Antibody suddenly does not work any longer, what can be the reason?
Can an antibody made to a full-length protein distinguish between 2 isoforms?
What is a reason for so called "ghost bands" to appear on blots?
One of the ways to remove background on a Western blot
How to predict in which technique a given antibody may work?
Which capture antibody to use in ELISA?
Why should we measure protein concentration?
Can results obtained in Western blot be representative for other techniques?
Effect of a membrane wash on background signal in Western blot
How to check predicted species reactivity of an antibody before purchase is done?
Which membrane to use for protein transfer?
How to visualize a target protein in a Western blot?
TBS or TBS-T, how using respective buffer for primary antibody incubation may change the outcome
What are the benefits of antibodies provided in lyophilized format?
What conditions to apply for detection of low abundance proteins?
Can primary antibodies be re-used?
How antibodies are validated - interviews with experts
Which secondary, fluorescent dye conjugated antibody to use with ECFP/FITC/TRIC filter set?
Advice for beginners and experienced users of Western blot technique
How to save time and shorten Western blot protocol?
How to transfer proteins of low molecular weight?
What are the benefits of using directly conjugated primary antibodies?
Antigen, immunogen and epitope, what is the difference?
How to get an intense signal in Western blot using a weak antibody?
How much of a specific antibody can be found in serum?
What does confirmed or predicted reactivity of an antibody mean?
What determines primary antibody dilution?
Affinity purified antibody, what does it really mean for the end user?
Can knowing the antigen sequence help identify background bands in a Western blot?
Best practices for Western Blot - interviews with experts
Store precious samples on a membrane instead of a freezer!
Save precious time in the lab, check antigen conservation before antibody purchase
Background signal and a sensitivity of ECL reagent
Tubulin alpha and gamma are not suitable as a loading control for metal stress
What can protein extraction buffer change in a Western blot?
Antibody validation strategies - crucial to secure antibody quality
What Western blot conditions to apply for high abundance proteins?
Same Western blot protocol for all antibodies?
Free antibody sample, is it always the best solution?
What to consider before antibody purchase?
Protein extraction, a key to success in Western blot
No signal on Western blot, what to do next?
Is sample dentaturation above 95°C, the golden standard?
Polyclonal Antibody in serum or affinity purified, what is a difference and how can it influence my results?
What are the most important factors for a successful antibody production?
Why is pre-immune serum screening of importance before antibody production starts?
Do secondary antibodies differ only in price?