Technical blog

2023-01-31
Can results obtained in Western blot be representative for other techniques?
2022-12-16
Effect of a membrane wash on background signal in Western blot
2022-12-15
How to check predicted species reactivity of an antibody before purchase is done?
2022-11-30
Which membrane to use for protein transfer?
2022-10-31
How to visualize a target protein in a Western blot?
2022-10-03
TBS or TBS-T, how using respective buffer for primary antibody incubation may change the outcome
2022-10-03
What are the benefits of antibodies provided in lyophilized format?
2022-09-09
What conditions to apply for detection of low abundance proteins?
2022-09-01
Can primary antibodies be re-used?
2022-08-10
How antibodies are validated - interviews with experts
2022-08-01
Which secondary, fluorescent dye conjugated antibody to use with ECFP/FITC/TRIC filter set?
2022-06-20
Advice for beginners and experienced users of Western blot technique
2022-05-16
How to save time and shorten Western blot protocol?
2022-03-21
How to transfer proteins of low molecular weight?
2022-03-16
What are the benefits of using directly conjugated primary antibodies?
2022-03-11
Antigen, immunogen and epitope, what is the difference?
2022-02-21
How to get an intense signal in Western blot using a weak antibody?
2022-01-19
How much of a specific antibody can be found in serum?
2021-11-26
What does confirmed or predicted reactivity of an antibody mean?
2021-11-15
What determines primary antibody dilution?
2021-11-10
Affinity purified antibody, what does it really mean for the end user?
2021-10-31
Can knowing the antigen sequence help identify background bands in a Western blot?
2021-08-18
Best practices for Western Blot - interviews with experts
2021-06-30
Store precious samples on a membrane instead of a freezer!
2021-05-29
Save precious time in the lab, check antigen conservation before antibody purchase
2021-04-30
Background signal and a sensitivity of ECL reagent
2021-03-17
Tubulin alpha and gamma are not suitable as a loading control for metal stress
2021-03-12
What can protein extraction buffer change in a Western blot?
2021-02-28
Antibody validation strategies - crucial to secure antibody quality
2021-01-13
What Western blot conditions to apply for high abundance proteins?
2020-12-18
Same Western blot protocol for all antibodies?
2020-11-01
Free antibody sample, is it always the best solution?
2020-10-12
What to consider before antibody purchase?
2020-09-30
Protein extraction, a key to success in Western blot
2020-08-31
No signal on Western blot, what to do next?
2020-07-27
Is sample dentaturation above 95°C, the golden standard?
2020-06-30
Polyclonal Antibody in serum or affinity purified, what is a difference and how can it influence my results?
2020-06-16
What are the most important factors for a successful antibody production?
2020-06-08
Why is pre-immune serum screening of importance before antibody production starts?
2020-06-02
Do secondary antibodies differ only in price?
2020-05-25
Is the antibody always to blame?
2020-05-10
TBS-T or PBS-T, does it matter which buffer is used for antibody incubations?
2020-05-07
What is a specific antibody concentration?
2020-04-23
FAQs about Catalog Antibodies
2020-04-23
FAQs about Antibody Production Process
2020-03-17
What is the safest method to store antibodies?
2020-01-20
Is an antibody not specific or Western blot protocol is not optimized?
2019-11-25
Common misconceptions about antibodies - II
2019-10-21
Common misconceptions about antibodies - I
2019-10-14
Why fractionate?
2019-03-22
How to cite the antibodies used in scientific publications?
2017-04-24
Save time! Use simultaneous incubation with few primary antibodies at the same time.
2017-02-16
Which secondary antibodies to use when my protein was immunoprecipiated using an antibody?
2016-05-13
Rapid Western blotting
2014-02-06
How to know that a commercial antibody is going to work on a protein from my species?
2013-09-13
Is a standarized reporting of research antibody use - a way to increase experimental reproducibility?
2010-12-02
Agrisera protein extraction buffer and antibodies used to validate high throughput protein extraction from tough marine phytoplankton