Technical tips

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Why an antibody may detect tagged protein but not endogenous one and in some cases endogenous protein but not its tagged version?
2025-10-30 Calclulated and aparent molecular weight of detected protein is different, why?
2025-10-09 How to chose right loading control for Western blot?
2025-10-06 Is an antibody going to work in a technique I am planning to use it in?
2025-09-30 Antibody reactivity to recombinant protein,does not validate antibody specificity in endogenous sample
2025-08-20 Can aggregated antibody be still used?
2025-08-15 Blot Once, Probe Twice - when such approach can be beneficial?
2025-07-31 Why should glycerol not be added, to stabilize antibodies in serum?
2025-06-27 Can methanol in transfer buffer be replaced by a less toxic alternative?
2025-05-12 Will the precipitation of SDS in the stripping buffer affect antibody stripping efficiency?
2025-04-29