Technical tips

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Same antibody, different outcome on samples from wide taxonomic range
2026-02-23 Can the quality of protein transfer be checked before the blot is developed with antibodies?
2026-01-16 Is wild type a wild type?
2025-12-29 Why an antibody may detect tagged protein but not endogenous one and in some cases endogenous protein but not its tagged version?
2025-10-30 Calclulated and aparent molecular weight of detected protein is different, why?
2025-10-09 How to chose right loading control for Western blot?
2025-10-06 Is an antibody going to work in a technique I am planning to use it in?
2025-09-30 Antibody reactivity to recombinant protein,does not validate antibody specificity in endogenous sample
2025-08-20 Can aggregated antibody be still used?
2025-08-15 Blot Once, Probe Twice - when such approach can be beneficial?
2025-07-31