Catalog and custom-produced Polyclonal Antibodies can be provided in different formats, such as: | ![]() 50 µg of antibody purified by affinity chromatography on Protein A or G will contain less specific antibodies than 50 µg of antibodies purified using matrix coupled with antigen (protein or peptide). For any type of conjugation with HRP, ALP or fluorophore, the antibody needs to be affinity purified on the specific antigen (used to produce it) to a specific immunoglobulin fraction. |
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Calclulated and aparent molecular weight of detected protein is different, why?2025-10-09 How to chose right loading control for Western blot?
2025-10-06 Calclulated and aparent molecular weight of detected protein is different, why?
2025-10-03 Is an antibody going to work in a technique I am planning to use it in?
2025-09-30 Antibody reactivity to recombinant protein,does not validate antibody specificity in endogenous sample
2025-08-20 Can aggregated antibody be still used?
2025-08-15 Blot Once, Probe Twice - when such approach can be beneficial?
2025-07-31 Why should glycerol not be added, to stabilize antibodies in serum?
2025-06-27 Can methanol in transfer buffer be replaced by a less toxic alternative?
2025-05-12 Will the precipitation of SDS in the stripping buffer affect antibody stripping efficiency?
2025-04-29
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