No. Secondary antibodies most importantly differ in respect to dillution they can be applied at. | ![]() Agrisera goat anti-rabbit IgG, HRP conjugated antibody (AS09 602) has been used in this Western blot with chemiluminescent detection. This antibody can be used in a minimum dilution of 1: 25 000 1h/RT. The full collection of Agrisera secondary antibodies can be found here. |
Latest
Why, to detect some proteins, 6M urea must be included in the extraction and loading buffers?2023-09-01 I have not used this antibody before, what conditions of Western blot do you recommend?
2023-07-27 Which subclasses of immunoglobulin G are found in rabbit serum?
2023-07-18 Antibody suddenly does not work any longer, what can be the reason?
2023-07-11 Can an antibody made to a full-length protein distinguish between 2 isoforms?
2023-05-10 What is a reason for so called "ghost bands" to appear on blots?
2023-03-30 One of the ways to remove background on a Western blot
2023-03-24 How to predict in which technique a given antibody may work?
2023-03-10 Which capture antibody to use in ELISA?
2023-03-06 Why should we measure protein concentration?
2023-03-03
Archive
- November - 2023
- September - 2023
- July - 2023
- May - 2023
- March - 2023
- January - 2023
- December - 2022
- November - 2022
- October - 2022
- September - 2022
- August - 2022
- June - 2022
- May - 2022
- March - 2022
- February - 2022
- January - 2022
- November - 2021
- October - 2021
- August - 2021
- June - 2021
- May - 2021
- April - 2021
- March - 2021
- February - 2021
- January - 2021
- December - 2020
- November - 2020
- October - 2020
- September - 2020
- August - 2020
- July - 2020
- June - 2020
- May - 2020
- April - 2020
- March - 2020
- January - 2020
- November - 2019
- October - 2019
- March - 2019
- April - 2017
- February - 2017
- May - 2016
- February - 2014
- September - 2013
- December - 2010