Q: The antibodies I have purchased are lyophilized. How should I reconstitute them? | ![]() A properly lyophilized antibody looks like a very light powder, which can be of different colours, depending on if it is serum (ligh pink), total IgG fraction (white) or antigen affinity purified antibody (white). Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Additional information about antibody storage can be found here. |
Latest
Calclulated and aparent molecular weight of detected protein is different, why?2025-10-09 How to chose right loading control for Western blot?
2025-10-06 Calclulated and aparent molecular weight of detected protein is different, why?
2025-10-03 Is an antibody going to work in a technique I am planning to use it in?
2025-09-30 Antibody reactivity to recombinant protein,does not validate antibody specificity in endogenous sample
2025-08-20 Can aggregated antibody be still used?
2025-08-15 Blot Once, Probe Twice - when such approach can be beneficial?
2025-07-31 Why should glycerol not be added, to stabilize antibodies in serum?
2025-06-27 Can methanol in transfer buffer be replaced by a less toxic alternative?
2025-05-12 Will the precipitation of SDS in the stripping buffer affect antibody stripping efficiency?
2025-04-29
Archive
- October - 2025
- September - 2025
- August - 2025
- July - 2025
- June - 2025
- May - 2025
- April - 2025
- March - 2025
- February - 2025
- January - 2025
- December - 2024
- November - 2024
- September - 2024
- July - 2024
- June - 2024
- May - 2024
- March - 2024
- February - 2024
- December - 2023
- November - 2023
- September - 2023
- July - 2023
- May - 2023
- March - 2023
- January - 2023
- December - 2022
- November - 2022
- October - 2022
- September - 2022
- August - 2022
- June - 2022
- May - 2022
- March - 2022
- February - 2022
- January - 2022
- November - 2021
- October - 2021
- August - 2021
- June - 2021
- May - 2021
- April - 2021
- March - 2021
- February - 2021
- January - 2021
- December - 2020
- November - 2020
- October - 2020
- September - 2020
- August - 2020
- July - 2020
- June - 2020
- May - 2020
- April - 2020
- March - 2020
- January - 2020
- November - 2019
- October - 2019
- March - 2019
- April - 2017
- February - 2017
- May - 2016
- February - 2014
- September - 2013
- December - 2010