Is wild type a wild type?

Chlamydomonas reinhardii is a model algal species and widely studied references organism, especially in research on photosynthesis. However, even though commonly used laboratory strains share the same ancestor, they differ by up to 50 000 nuclear variants, changes which occurred during laboratory propagation.

In the recent publication by Liu et al. (2025). Hidden Diversity: Transcriptomic and Photosynthetic Variation Among Common" Wild Type" Chlamydomonas Strains. Plant J. 2025 Dec;124(5):e70615.doi: 10.1111/tpj.70615 the authors are showing the data for 40 % of genes being differentially expressed between common Chlamydomonas reinhardtii laboratory wild type strains and catalogued a range of strain-dependent responses on the physiological level, related to photosynthesis.

Final conclusion: wilt type strain-to-strain variation in all photosynthetic parameters, should not be overlooked when planning specific experiments.

Agrisera antibody employed in the experiments was: Anti-LHCSR3,,AS14 2766.

As seen in this example one and same antibody can detect recombinant protein with higer intensity, compared to endogenous not tagged protein. The reason is not equal abundance of both proteins.

Is wild type a wild type?

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