Can aggregated antibody be still used?

Antibody aggregation may happen during:

  • reconstitution from lyophilized format
  • prolonged storage

Before any antibody is used in Western blot or another application, the solution should be quickly checked if no aggregates are formed.

The reasons for antibody precipitation in the solution are following: 

vigorous vortexing and pippeting
  • Freeze-thaw cycles
  • temperature changes
  • pH near isoelectric point
  • lack of additives like glycerol or other compounds which prevent aggregation

For catalogue antibodies, always check product information sheet on recommended reconstitution and storage conditions.



   Aggregated antibody
 Technical tips

Technical tips

Latest

What are the most common misconceptionsa about primary antibodies?
2026-05-27 What are the most common mistakes done with secondary antibodies?
2026-05-26 Simple extraction protocol for membrane proteins
2026-04-17 How to improve signal with a weak antibody?
2026-03-26 Same antibody, different outcome on samples from wide taxonomic range
2026-02-23 Can the quality of protein transfer be checked before the blot is developed with antibodies?
2026-01-16 Is wild type a wild type?
2025-12-29 Why an antibody may detect tagged protein but not endogenous one and in some cases endogenous protein but not its tagged version?
2025-10-30 Calclulated and aparent molecular weight of detected protein is different, why?
2025-10-09 How to chose right loading control for Western blot?
2025-10-06

Archive


Close
Login / Retailer
Register
Forgot password?