Why bands on the blot are not distinct from each other?

Q: Why can I not see separate bands of a target protein in my samples, but all look like one band on the blot?

A: There can be few reasons, as:

Protein load/well could have been too high, which led to bands merging together, making them difficult to distinguish from each other.
Proteins could form aggregates or complexes, leading to poor band separation.

Remedy: decrease protein load/well and use less sensitive detection reagent.

Why bands on the blot can not be distinguished from each other?

Agrisera detailed Western blot protocol

Questions?
Please contact us!

Technical blog

Technical blog

Latest

Which isotype is a rabbit polyclonal antibody?
2024-05-06 Why bands on the blot are not distinct from each other?
2024-05-02 What parameters should be consider when transferring HMW proteins?
2024-03-01 Why some antibodies have a broad species reactivity, while the others do not?
2024-02-20 Are directly conjugated primary antibodies always the best choice?
2023-12-28 How to obtain sharper protein bands in Western blot?
2023-12-20 Are monoclonal antibodies better, compared to polyclonal antibodies?
2023-11-30 Why, to detect some proteins, 6M urea must be included in the extraction and loading buffers?
2023-09-01 I have not used this antibody before, what conditions of Western blot do you recommend?
2023-07-27 Which subclasses of immunoglobulin G are found in rabbit serum?
2023-07-18

Login / RetailerClose

Forgot password?