PsbF | beta subunit of Cytochrome b559 of PSII

Product no: AS06 113

AS06 113 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, P.sativum, T. aestivum

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from plant PsbF sequences including Arabidopsis thaliana P62095, AtCg00570

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 200 µl
    Reconstitution: For reconstitution add 200 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW:

    4 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Pisum sativum, Triticum aestivum
    Predicted reactivity: Cannabis sativa, Marchantia polymorpha, Nicotiana tabacum, Pinus sylvestris, Populus tremula
    Species of your interest not listed? Contact us
    Not reactive in:

    Chlamydomonas reinhardtii, cyanobacteria

  • Application Examples
  • Application example

    5µg protein of Arabidopsis thaliana (1) leaf, extracted with PEB (AS08 300), (2) chloroplasts (normal light), (3) thylakoids (extracted from 2), (4) chloroplasts (high light), (5) thylakoids (extracted from 4), (6) Photosystem I, and (7) BBY particles (enriched in Photosystem II) from pea were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1.5h (30V) to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with AS06 113 (1:1000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with chemiluminescent detection reagent, using a Fuji LAS-3000 CCD (150s, high sensitivity). The PsbF protein is detected with an apparent mass of < 10 kDa
    With this protocol, there is a cross-reaction with a target at ~43 kDa.

  • Additional Information
  • Additional information: This product can be sold containing ProClin if requested
    Additional information (application): This antibody works better on thylakoid and chloroplast fractions than on a total cell extract
  • Background
  • Background:

    Cytochrome b559 (Cyt b559) is encoded by the chloroplast genes psbE and psbF and is comprised of two low molecular mass polypeptides, α and ß subunits, with molecular masses of 9 and 4 kDa, respectively. The Cyt b559 is closely associated with PSII in all oxygenic photosynthetic organisms. The α and ß subunits of the Cyt b559 are components of the minimal PSII reaction center complex that is still capable of primary charge separation In summary, both PsbE and PsbF are essential components for PSII assembly, and they are probably involved in electron transport mechanisms that help to protect PSII from photodamage.

  • Product Citations
  • Selected references: Hackett et al. (2017). An Organelle RNA Recognition Motif Protein Is Required for Photosystem II Subunit psbF Transcript Editing. Plant Physiol. 2017
    Yang-Er Chen et al. (2017). Responses of photosystem II and antioxidative systems to high light and high temperature co-stress in wheat. J. of Exp. Botany, Volume 135, March 2017, Pages 45–55.
    Nishimura et al. (2016). The N-terminal sequence of the extrinsic PsbP protein modulates the redox potential of Cyt b559 in photosystem II. Sci Rep. 2016 Feb 18;6:21490. doi: 10.1038/srep21490.
    Lucinski et al. (2011). Involvement of Deg5 protease in wounding-related disposal of PsbF apoprotein. Plant Physiol Biochem. 49(3):311-20.
    Garcia-Cerdan et al. (2008). Antisense inhibition of the PsbX protein affects PSII integrity in the higher plant Arabidopsis thaliana. Plant Cell Physiol 50: 191-202
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts
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