KLH | Keyhole limpet hemocyanin

Name: KLH | Keyhole limpet hemocyanin
SKU: AS99 001
Price: 299 EUR
Availability: InStock

AS99 001 | Clonality: Polyclonal | Host: Rabbit | Reactivity: keyhole limpet hemocyanin (KLH), whole molecule

KLH | Keyhole limpet hemocyanin in the group Antibodies Human Cell Biology / Carrier Proteins at Agrisera AB (Antibodies for research) (AS99 001)

Product Information

Immunogen

Purified keyhole limpet hemocyanin (KLH), whole molecule,

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunolocalization (IL), Western blot (WB)

Recommended dilution

1: 10 000 (ELISA), 1: 10 000 (WB), 1: 1000 (IL)

Expected | apparent MW ca, 400 kDa/subunit

Reactivity

Confirmed reactivity Megathura crenulata - most commonly used carrier protein

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Protein present in plant vascular tissue (xylem and vascular cambium) is detected by anti-KLH antibodies (Höglund et al. 2002) which might lead to false results in IL when using anti-peptide antibodies generated to KLH-conjugated peptide.

Antibody can be used as a negative control to determine if observed signal is generated by anti-KLH or anti-peptide antibodies, Due to its large size KLH protein will be very difficult to separate on SDS-PAGE

Background

Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host’s immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.

Product citations

Selected references Geadkaew et al. (2014). Bi-functionality of Opisthorchis viverrini aquaporins. doi:10.1016/j.biochi.2014.11.013.
Höglund et al. (2002). An Antigen Expressed During Plant Vascular Development Crossreacts with Antibodies Towards KLH (Keyhole Limpet Hemocyanin). J of Histochem & Cytochem. 50:999-1003.

immunogen:	Purified keyhole limpet hemocyanin (KLH), whole molecule,
Reconstitution:	For reconstitution add 50 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	50 µg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Immunolocalization (IL), Western blot (WB)
recommended dilution:	1: 10 000 (ELISA), 1: 10 000 (WB), 1: 1000 (IL)
calculated \| apparent molecular mass [kDa]:	ca, 400 kDa/subunit
Confirmed reactivity:	Megathura crenulata - most commonly used carrier protein
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	Protein present in plant vascular tissue (xylem and vascular cambium) is detected by anti-KLH antibodies (Höglund et al. 2002) which might lead to false results in IL when using anti-peptide antibodies generated to KLH-conjugated peptide.
additional information (application):	Antibody can be used as a negative control to determine if observed signal is generated by anti-KLH or anti-peptide antibodies, Due to its large size KLH protein will be very difficult to separate on SDS-PAGE
background:	Keyhole limpet hemocyanin (KLH) is a large cooper-containing protein consisting of subunits with MW of 400 kDa. It is found in the hemolymph of the sea mollusk Megathura crenulata. This extracellular respiratory protein has many immunostimulatory properties, including the ability to enhance the host’s immune response by interacting with T cells, monocytes, macrophages, and polymorphonuclear lymphocytes. Since its discovery, KLH has been used primarily as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.
All references:	Geadkaew et al. (2014). Bi-functionality of Opisthorchis viverrini aquaporins. doi:10.1016/j.biochi.2014.11.013. Höglund et al. (2002). An Antigen Expressed During Plant Vascular Development Crossreacts with Antibodies Towards KLH (Keyhole Limpet Hemocyanin). J of Histochem & Cytochem. 50:999-1003.