Protocols
Plant organelle/membrane isolation
Purification
Plant organelle/membrane isolation
- Mitochondrial fraction
- Nuclear fraction
- Plasma membrane fraction
- Thylakoid extraction
- Arabidopsis thylakoid extraction
- Arabidopsis lumen extraction
- BBY preparation
- Chlorophyll measurements
- PSII RC extraction for cryo-EM
- Intact chloroplast isolation method
- Extraction of leaf proteins
- Diatom protein extraction
- Phenol protein extraction
- Protein extraction from grasses
- Ponceau membrane staining
- TCA acetone precipitation method
- Collection of articles
- Western blot protocol
- Western blot video tutorial
- Peptide neutralization/competition assay
- Simultaneous Western blot
- Quantitative Western blot
- Quantitative Western blot video tutorial
- Western blot troubleshooting
- Western blot using IgY
- Western blot in denatured conditions (urea gel)
- Dot blot
- ELISA
- Immunoprecipitation
- Immunoprecipitation/IgY
- Immunohistochemistry
- Anti-KLH antibody removal
- Yolk delipidation
- Meiotic staining
Technical information
Antibody typesPurification
- Antibody purification
- Antibody purification - small amount of protein
- IgY purification methods
- Protein purification using antibodies
- Elution of antibodies from affinity columns
Protocols > Western blot video tutorial In this three-part online workshop, you’ll gain expert insights into every step of the process — from sample preparation and antibody selection to blocking, detection, and confident result interpretation. Learn proven strategies and practical tips you can apply immediately to achieve cleaner, more reliable blots. This workshop was held in 2025 in collaboration with Lucerna-Chem. If you would like to receive the slides of this presentation in pdf format or ppt format for teaching purposes, please contact: [email protected]. Part I – Western blot tips and techniques |
