Protocols
Plant organelle/membrane isolation
Purification
Plant organelle/membrane isolation
- Arabidopsis lumen extraction
- Arabidopsis thylakoid extraction
- BBY preparation
- Chlorophyll measurements
- Intact chloroplast isolation method
- Mitochondrial fraction
- Nuclear fraction
- Plasma membrane fraction
- PSII RC extraction for cryo-EM
- Thylakoid extraction
- Vacuol isolation
- Collection of articles
- Diatom protein extraction
- Extraction of leaf proteins
- Phenol protein extraction
- Ponceau membrane staining
- Protein extraction from grasses
- TCA acetone precipitation method
- Western blot protocol
- Western blot video tutorial
- Western blot troubleshooting
- Western blot using IgY
- Western blot in denatured conditions (urea gel)
- Peptide neutralization/competition assay
- Quantitative Western blot
- Quantitative Western blot video tutorial
- Simultaneous Western blot
- Anti-KLH antibody removal
- Dot blot
- ELISA
- Immunohistochemistry
- Immunoprecipitation
- Immunoprecipitation/IgY
- Meiotic staining
- Yolk delipidation
Technical information
Antibody typesPurification
- Antibody purification
- Antibody purification - small amount of protein
- Elution of antibodies from affinity columns
- IgY purification methods
- Protein purification using antibodies
Protocols > Western blot video tutorial In this three-part online workshop, you’ll gain expert insights into every step of the process — from sample preparation and antibody selection to blocking, detection, and confident result interpretation. Learn proven strategies and practical tips you can apply immediately to achieve cleaner, more reliable blots. This workshop was held in 2025 in collaboration with Lucerna-Chem. If you would like to receive the slides of this presentation in pdf format or ppt format for teaching purposes, please contact: [email protected]. Part I – Western blot tips and techniques |
