Plant organelle/membrane isolation
- Mitochondrial fraction
- Nuclear fraction
- Plasma membrane fraction
- Thylakoid extraction
- Arabidopsis thylakoid preparation
- Arabidopsis thylakoid extraction
- Arabidopsis lumen extraction
- BBY preparation
- Chlorophyll measurements
- PSII RC extraction for cryo-EM
- Extraction of leaf proteins
- Diatom protein extraction
- Phenol protein extraction
- Protein extraction from grasses
- Ponceau membrane staining
- TCA acetone precipitation method
- Western blot protocol
- Western blot video tutorial
- Peptide neutralization/competition assay
- Simultaneous Western blot
- Quantitative Western blot
- Quantitative Western blot video tutorial
- Western blot troubleshooting
- Western blot using IgY
- Dot blot
- ELISA
- Immunoprecipitation
- Immunoprecipitation/IgY
- Immunohistochemistry
- Anti-KLH antibody removal
- Yolk delipidation
Technical information
Antibody typesPurification
- Antibody purification
- Antibody purification - small amount of protein
- IgY purification methods
- Protein purification using antibodies
- Elution of antibodies from affinity columns
Protocols > ImmunohistochemistryGeneral commentsImmunohistochemistry demands keeping a gentle balance between exposure of antigenic sites and preservation of structure. Some treatments might not be enough to reveal antigenic determinants, recognized by antibodies. Other treatments can simply wipe them off. Therefore, some adjustment of fixing technique is necessary for good results.In case of problems with increased background
Overview of steps in procedureTissue fixing
For both primary and secondary antibodies. |